A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats

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A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats. / Filip, R.; Harrison, Adrian Paul; Pierzynowski, S.G.

I: American Journal of Pharmacology and Toxicology, Bind 2, Nr. 4, 2008, s. 170-177.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Filip, R, Harrison, AP & Pierzynowski, SG 2008, 'A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats', American Journal of Pharmacology and Toxicology, bind 2, nr. 4, s. 170-177.

APA

Filip, R., Harrison, A. P., & Pierzynowski, S. G. (2008). A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats. American Journal of Pharmacology and Toxicology, 2(4), 170-177.

Vancouver

Filip R, Harrison AP, Pierzynowski SG. A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats. American Journal of Pharmacology and Toxicology. 2008;2(4):170-177.

Author

Filip, R. ; Harrison, Adrian Paul ; Pierzynowski, S.G. / A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats. I: American Journal of Pharmacology and Toxicology. 2008 ; Bind 2, Nr. 4. s. 170-177.

Bibtex

@article{ea81c250a1c311ddb6ae000ea68e967b,
title = "A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats",
abstract = "This study has evaluated the effect of phytohaemagglutinin (PHA) in combination with alpha-ketoglutaric acid (AKG), on GI-tract morphology and N balance in adult rats. Rats, aged approx. 15 months, were assigned to one of four experimental groups, (1) Control group, (2) AKG group, (3) AKG+PHA 100% group and (4) AKG+PHA 1% group. AKG and AKG+PHA were administered via a stomach tube. Rats were treated for a period of 7 days before being killed humanely. Lighter GI-tract weights were found in the AKG and AKG+PHA 1% groups (6.8%, p<0.05 and p<0.01, respectively) compared to that of Control rats, whilst AKG+PHA 100% treatment resulted in no such loss of GI-tract total weight. Interestingly, AKG, AKG+PHA 1% and AKG+PHA treatment resulted in a significant 114% (p<0.05), 116% (p<0.001) and a 145% (p<0.001), increase in duodenal crypt depth, respectively. Moreover, AKG, AKG+PHA 1% and AKG+PHA 100% treatment induced a 107% (p<0.05), 109% (p<0.001) and a 119% (p<0.001), increase in the thickness of the tunica mucosa of the proximal GI-tract, respectively. However, whilst there was a trend towards a reduction in N excretion in urine for the AKG+PHA 100% group, compared to that of the Controls (14.3% lower), this difference was not found to be statistically significant. In conclusion, a combination of PHA and AKG treatment (AKG+PHA 100%) stimulates proliferation of GI-tract crypt depth and tunica mucosa thickness cf. that of Control rats, findings that would be of benefit to the elderly and to individuals suffering from drug induced GI-tract erosion and injury.",
keywords = "Former LIFE faculty, AKG, PHA, GI-tract, ageing",
author = "R. Filip and Harrison, {Adrian Paul} and S.G. Pierzynowski",
year = "2008",
language = "English",
volume = "2",
pages = "170--177",
journal = "American Journal of Pharmacology and Toxicology",
issn = "1557-4962",
publisher = "Science Publications",
number = "4",

}

RIS

TY - JOUR

T1 - A combined phytohemagglutinin and a-ketoglutarate pharmacology study of gut morphology and growth in older adult rats

AU - Filip, R.

AU - Harrison, Adrian Paul

AU - Pierzynowski, S.G.

PY - 2008

Y1 - 2008

N2 - This study has evaluated the effect of phytohaemagglutinin (PHA) in combination with alpha-ketoglutaric acid (AKG), on GI-tract morphology and N balance in adult rats. Rats, aged approx. 15 months, were assigned to one of four experimental groups, (1) Control group, (2) AKG group, (3) AKG+PHA 100% group and (4) AKG+PHA 1% group. AKG and AKG+PHA were administered via a stomach tube. Rats were treated for a period of 7 days before being killed humanely. Lighter GI-tract weights were found in the AKG and AKG+PHA 1% groups (6.8%, p<0.05 and p<0.01, respectively) compared to that of Control rats, whilst AKG+PHA 100% treatment resulted in no such loss of GI-tract total weight. Interestingly, AKG, AKG+PHA 1% and AKG+PHA treatment resulted in a significant 114% (p<0.05), 116% (p<0.001) and a 145% (p<0.001), increase in duodenal crypt depth, respectively. Moreover, AKG, AKG+PHA 1% and AKG+PHA 100% treatment induced a 107% (p<0.05), 109% (p<0.001) and a 119% (p<0.001), increase in the thickness of the tunica mucosa of the proximal GI-tract, respectively. However, whilst there was a trend towards a reduction in N excretion in urine for the AKG+PHA 100% group, compared to that of the Controls (14.3% lower), this difference was not found to be statistically significant. In conclusion, a combination of PHA and AKG treatment (AKG+PHA 100%) stimulates proliferation of GI-tract crypt depth and tunica mucosa thickness cf. that of Control rats, findings that would be of benefit to the elderly and to individuals suffering from drug induced GI-tract erosion and injury.

AB - This study has evaluated the effect of phytohaemagglutinin (PHA) in combination with alpha-ketoglutaric acid (AKG), on GI-tract morphology and N balance in adult rats. Rats, aged approx. 15 months, were assigned to one of four experimental groups, (1) Control group, (2) AKG group, (3) AKG+PHA 100% group and (4) AKG+PHA 1% group. AKG and AKG+PHA were administered via a stomach tube. Rats were treated for a period of 7 days before being killed humanely. Lighter GI-tract weights were found in the AKG and AKG+PHA 1% groups (6.8%, p<0.05 and p<0.01, respectively) compared to that of Control rats, whilst AKG+PHA 100% treatment resulted in no such loss of GI-tract total weight. Interestingly, AKG, AKG+PHA 1% and AKG+PHA treatment resulted in a significant 114% (p<0.05), 116% (p<0.001) and a 145% (p<0.001), increase in duodenal crypt depth, respectively. Moreover, AKG, AKG+PHA 1% and AKG+PHA 100% treatment induced a 107% (p<0.05), 109% (p<0.001) and a 119% (p<0.001), increase in the thickness of the tunica mucosa of the proximal GI-tract, respectively. However, whilst there was a trend towards a reduction in N excretion in urine for the AKG+PHA 100% group, compared to that of the Controls (14.3% lower), this difference was not found to be statistically significant. In conclusion, a combination of PHA and AKG treatment (AKG+PHA 100%) stimulates proliferation of GI-tract crypt depth and tunica mucosa thickness cf. that of Control rats, findings that would be of benefit to the elderly and to individuals suffering from drug induced GI-tract erosion and injury.

KW - Former LIFE faculty

KW - AKG

KW - PHA

KW - GI-tract

KW - ageing

M3 - Journal article

VL - 2

SP - 170

EP - 177

JO - American Journal of Pharmacology and Toxicology

JF - American Journal of Pharmacology and Toxicology

SN - 1557-4962

IS - 4

ER -

ID: 8105584