Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates

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Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates. / Hummelshøj, Tina; Nissen, Janna; Munthe-Fog, Lea; Koch, Claus; Bertelsen, Mads Frost; Garred, Peter.

I: P L o S One, Bind 6, Nr. 12, 2011, s. e28187.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Hummelshøj, T, Nissen, J, Munthe-Fog, L, Koch, C, Bertelsen, MF & Garred, P 2011, 'Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates', P L o S One, bind 6, nr. 12, s. e28187. https://doi.org/10.1371/journal.pone.0028187

APA

Hummelshøj, T., Nissen, J., Munthe-Fog, L., Koch, C., Bertelsen, M. F., & Garred, P. (2011). Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates. P L o S One, 6(12), e28187. https://doi.org/10.1371/journal.pone.0028187

Vancouver

Hummelshøj T, Nissen J, Munthe-Fog L, Koch C, Bertelsen MF, Garred P. Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates. P L o S One. 2011;6(12):e28187. https://doi.org/10.1371/journal.pone.0028187

Author

Hummelshøj, Tina ; Nissen, Janna ; Munthe-Fog, Lea ; Koch, Claus ; Bertelsen, Mads Frost ; Garred, Peter. / Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates. I: P L o S One. 2011 ; Bind 6, Nr. 12. s. e28187.

Bibtex

@article{3aa2b6abf4614549a0ece3f9cbc976a4,
title = "Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates",
abstract = "The ficolins recognize carbohydrates and acetylated compounds on microorganisms and dying host cells and are able to activate the lectin pathway of the complement system. In humans, three ficolin genes have been identified: FCN1, FCN2 and FCN3, which encode ficolin-1, ficolin-2 and ficolin-3, respectively. Rodents have only two ficolins designated ficolin-A and ficolin-B that are closely related to human ficolin-1, while the rodent FCN3 orthologue is a pseudogene. Ficolin-2 and ficolin-3 have so far only been observed in humans. Thus, we performed a systematic investigation of the FCN genes in non-human primates. The exons and intron-exon boundaries of the FCN1-3 genes were sequenced in the following primate species: chimpanzee, gorilla, orangutan, rhesus macaque, cynomolgus macaque, baboon and common marmoset. We found that the exon organisation of the FCN genes was very similar between all the non-human primates and the human FCN genes. Several variations in the FCN genes were found in more than one primate specie suggesting that they were carried from one species to another including humans. The amino acid diversity of the ficolins among human and non-human primate species was estimated by calculating the Shannon entropy revealing that all three proteins are generally highly conserved. Ficolin-1 and ficolin-2 showed the highest diversity, whereas ficolin-3 was more conserved. Ficolin-2 and ficolin-3 were present in non-human primate sera with the same characteristic oligomeric structures as seen in human serum. Taken together all the FCN genes show the same characteristics in lower and higher primates. The existence of trans-species polymorphisms suggests that different FCN allelic lineages may be passed from ancestral to descendant species.",
keywords = "Former LIFE faculty",
author = "Tina Hummelsh{\o}j and Janna Nissen and Lea Munthe-Fog and Claus Koch and Bertelsen, {Mads Frost} and Peter Garred",
year = "2011",
doi = "10.1371/journal.pone.0028187",
language = "English",
volume = "6",
pages = "e28187",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

RIS

TY - JOUR

T1 - Allelic lineages of the ficolin genes (FCNs) are passed from ancestral to descendant primates

AU - Hummelshøj, Tina

AU - Nissen, Janna

AU - Munthe-Fog, Lea

AU - Koch, Claus

AU - Bertelsen, Mads Frost

AU - Garred, Peter

PY - 2011

Y1 - 2011

N2 - The ficolins recognize carbohydrates and acetylated compounds on microorganisms and dying host cells and are able to activate the lectin pathway of the complement system. In humans, three ficolin genes have been identified: FCN1, FCN2 and FCN3, which encode ficolin-1, ficolin-2 and ficolin-3, respectively. Rodents have only two ficolins designated ficolin-A and ficolin-B that are closely related to human ficolin-1, while the rodent FCN3 orthologue is a pseudogene. Ficolin-2 and ficolin-3 have so far only been observed in humans. Thus, we performed a systematic investigation of the FCN genes in non-human primates. The exons and intron-exon boundaries of the FCN1-3 genes were sequenced in the following primate species: chimpanzee, gorilla, orangutan, rhesus macaque, cynomolgus macaque, baboon and common marmoset. We found that the exon organisation of the FCN genes was very similar between all the non-human primates and the human FCN genes. Several variations in the FCN genes were found in more than one primate specie suggesting that they were carried from one species to another including humans. The amino acid diversity of the ficolins among human and non-human primate species was estimated by calculating the Shannon entropy revealing that all three proteins are generally highly conserved. Ficolin-1 and ficolin-2 showed the highest diversity, whereas ficolin-3 was more conserved. Ficolin-2 and ficolin-3 were present in non-human primate sera with the same characteristic oligomeric structures as seen in human serum. Taken together all the FCN genes show the same characteristics in lower and higher primates. The existence of trans-species polymorphisms suggests that different FCN allelic lineages may be passed from ancestral to descendant species.

AB - The ficolins recognize carbohydrates and acetylated compounds on microorganisms and dying host cells and are able to activate the lectin pathway of the complement system. In humans, three ficolin genes have been identified: FCN1, FCN2 and FCN3, which encode ficolin-1, ficolin-2 and ficolin-3, respectively. Rodents have only two ficolins designated ficolin-A and ficolin-B that are closely related to human ficolin-1, while the rodent FCN3 orthologue is a pseudogene. Ficolin-2 and ficolin-3 have so far only been observed in humans. Thus, we performed a systematic investigation of the FCN genes in non-human primates. The exons and intron-exon boundaries of the FCN1-3 genes were sequenced in the following primate species: chimpanzee, gorilla, orangutan, rhesus macaque, cynomolgus macaque, baboon and common marmoset. We found that the exon organisation of the FCN genes was very similar between all the non-human primates and the human FCN genes. Several variations in the FCN genes were found in more than one primate specie suggesting that they were carried from one species to another including humans. The amino acid diversity of the ficolins among human and non-human primate species was estimated by calculating the Shannon entropy revealing that all three proteins are generally highly conserved. Ficolin-1 and ficolin-2 showed the highest diversity, whereas ficolin-3 was more conserved. Ficolin-2 and ficolin-3 were present in non-human primate sera with the same characteristic oligomeric structures as seen in human serum. Taken together all the FCN genes show the same characteristics in lower and higher primates. The existence of trans-species polymorphisms suggests that different FCN allelic lineages may be passed from ancestral to descendant species.

KW - Former LIFE faculty

U2 - 10.1371/journal.pone.0028187

DO - 10.1371/journal.pone.0028187

M3 - Journal article

VL - 6

SP - e28187

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 12

ER -

ID: 37609180