Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes

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Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes. / Kudirkiene, Egle; Bager, Ragnhild Jørgensen; Johnson, Timothy J.; Bojesen, Anders Miki.

I: B M C Genomics, Bind 15, 1093, 2014.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Kudirkiene, E, Bager, RJ, Johnson, TJ & Bojesen, AM 2014, 'Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes', B M C Genomics, bind 15, 1093. https://doi.org/10.1186/1471-2164-15-1093

APA

Kudirkiene, E., Bager, R. J., Johnson, T. J., & Bojesen, A. M. (2014). Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes. B M C Genomics, 15, [1093]. https://doi.org/10.1186/1471-2164-15-1093

Vancouver

Kudirkiene E, Bager RJ, Johnson TJ, Bojesen AM. Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes. B M C Genomics. 2014;15. 1093. https://doi.org/10.1186/1471-2164-15-1093

Author

Kudirkiene, Egle ; Bager, Ragnhild Jørgensen ; Johnson, Timothy J. ; Bojesen, Anders Miki. / Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes. I: B M C Genomics. 2014 ; Bind 15.

Bibtex

@article{d3d13257d7b1498c897a1cb0400a5073,
title = "Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes",
abstract = "BackgroundFimbriae are bacterial cell surface organelles involved in the pathogenesis of many bacterial species, including Gallibacterium anatis, in which a F17-like fimbriae of the chaperone-usher (CU) family was recently shown to be an important virulence factor and vaccine candidate. To reveal the distribution and variability of CU fimbriae 22 genomes of the avian host-restricted bacteria Gallibacterium spp. were investigated. Fimbrial clusters were classified using phylogeny-based and conserved domain (CD) distribution-based approaches. To characterize the fimbriae in depth evolutionary analysis and in vitro expression of the most prevalent fimbrial clusters was performed.ResultsOverall 48 CU fimbriae were identified in the genomes of the examined Gallibacterium isolates. All fimbriae were assigned to γ4 clade of the CU fimbriae of Gram-negative bacteria and were organized in four-gene clusters encoding a putative major fimbrial subunit, a chaperone, an usher and a fimbrial adhesin. Five fimbrial clusters (Flf-Flf4) and eight conserved domain groups were defined to accommodate the identified fimbriae. Although, the number of different fimbrial clusters in individual Gallibacterium genomes was low, there was substantial amino acid sequence variability in the major fimbrial subunit and the adhesin proteins. The distribution of CDs among fimbrial clusters, analysis of their flanking regions, and evolutionary comparison of the strains revealed that Gallibacterium fimbrial clusters likely underwent evolutionary divergence resulting in highly host adapted and antigenically variable fimbriae. In vitro, only the fimbrial subunit FlfA was expressed in most Gallibacterium strains encoding this protein. The absence or scarce expression of the two other common fimbrial subunits (Flf1A and Flf3A) indicates that their expression may require other in vitro or in vivo conditions.ConclusionsThis is the first approach establishing a systematic fimbria classification system within Gallibacterium spp., which indicates a species-wide distribution of γ4 CU fimbriae among a diverse collection of Gallibacterium isolates. The expression of only one out of up to three fimbriae present in the individual genomes in vitro suggests that fimbriae expression in Gallibacterium is highly regulated. This information is important for future attempts to understand the role of Gallibacterium fimbriae in pathogenesis, and may prove useful for improved control of Gallibacterium infections in chickens.",
author = "Egle Kudirkiene and Bager, {Ragnhild J{\o}rgensen} and Johnson, {Timothy J.} and Bojesen, {Anders Miki}",
year = "2014",
doi = "10.1186/1471-2164-15-1093",
language = "English",
volume = "15",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes

AU - Kudirkiene, Egle

AU - Bager, Ragnhild Jørgensen

AU - Johnson, Timothy J.

AU - Bojesen, Anders Miki

PY - 2014

Y1 - 2014

N2 - BackgroundFimbriae are bacterial cell surface organelles involved in the pathogenesis of many bacterial species, including Gallibacterium anatis, in which a F17-like fimbriae of the chaperone-usher (CU) family was recently shown to be an important virulence factor and vaccine candidate. To reveal the distribution and variability of CU fimbriae 22 genomes of the avian host-restricted bacteria Gallibacterium spp. were investigated. Fimbrial clusters were classified using phylogeny-based and conserved domain (CD) distribution-based approaches. To characterize the fimbriae in depth evolutionary analysis and in vitro expression of the most prevalent fimbrial clusters was performed.ResultsOverall 48 CU fimbriae were identified in the genomes of the examined Gallibacterium isolates. All fimbriae were assigned to γ4 clade of the CU fimbriae of Gram-negative bacteria and were organized in four-gene clusters encoding a putative major fimbrial subunit, a chaperone, an usher and a fimbrial adhesin. Five fimbrial clusters (Flf-Flf4) and eight conserved domain groups were defined to accommodate the identified fimbriae. Although, the number of different fimbrial clusters in individual Gallibacterium genomes was low, there was substantial amino acid sequence variability in the major fimbrial subunit and the adhesin proteins. The distribution of CDs among fimbrial clusters, analysis of their flanking regions, and evolutionary comparison of the strains revealed that Gallibacterium fimbrial clusters likely underwent evolutionary divergence resulting in highly host adapted and antigenically variable fimbriae. In vitro, only the fimbrial subunit FlfA was expressed in most Gallibacterium strains encoding this protein. The absence or scarce expression of the two other common fimbrial subunits (Flf1A and Flf3A) indicates that their expression may require other in vitro or in vivo conditions.ConclusionsThis is the first approach establishing a systematic fimbria classification system within Gallibacterium spp., which indicates a species-wide distribution of γ4 CU fimbriae among a diverse collection of Gallibacterium isolates. The expression of only one out of up to three fimbriae present in the individual genomes in vitro suggests that fimbriae expression in Gallibacterium is highly regulated. This information is important for future attempts to understand the role of Gallibacterium fimbriae in pathogenesis, and may prove useful for improved control of Gallibacterium infections in chickens.

AB - BackgroundFimbriae are bacterial cell surface organelles involved in the pathogenesis of many bacterial species, including Gallibacterium anatis, in which a F17-like fimbriae of the chaperone-usher (CU) family was recently shown to be an important virulence factor and vaccine candidate. To reveal the distribution and variability of CU fimbriae 22 genomes of the avian host-restricted bacteria Gallibacterium spp. were investigated. Fimbrial clusters were classified using phylogeny-based and conserved domain (CD) distribution-based approaches. To characterize the fimbriae in depth evolutionary analysis and in vitro expression of the most prevalent fimbrial clusters was performed.ResultsOverall 48 CU fimbriae were identified in the genomes of the examined Gallibacterium isolates. All fimbriae were assigned to γ4 clade of the CU fimbriae of Gram-negative bacteria and were organized in four-gene clusters encoding a putative major fimbrial subunit, a chaperone, an usher and a fimbrial adhesin. Five fimbrial clusters (Flf-Flf4) and eight conserved domain groups were defined to accommodate the identified fimbriae. Although, the number of different fimbrial clusters in individual Gallibacterium genomes was low, there was substantial amino acid sequence variability in the major fimbrial subunit and the adhesin proteins. The distribution of CDs among fimbrial clusters, analysis of their flanking regions, and evolutionary comparison of the strains revealed that Gallibacterium fimbrial clusters likely underwent evolutionary divergence resulting in highly host adapted and antigenically variable fimbriae. In vitro, only the fimbrial subunit FlfA was expressed in most Gallibacterium strains encoding this protein. The absence or scarce expression of the two other common fimbrial subunits (Flf1A and Flf3A) indicates that their expression may require other in vitro or in vivo conditions.ConclusionsThis is the first approach establishing a systematic fimbria classification system within Gallibacterium spp., which indicates a species-wide distribution of γ4 CU fimbriae among a diverse collection of Gallibacterium isolates. The expression of only one out of up to three fimbriae present in the individual genomes in vitro suggests that fimbriae expression in Gallibacterium is highly regulated. This information is important for future attempts to understand the role of Gallibacterium fimbriae in pathogenesis, and may prove useful for improved control of Gallibacterium infections in chickens.

U2 - 10.1186/1471-2164-15-1093

DO - 10.1186/1471-2164-15-1093

M3 - Journal article

C2 - 25495603

VL - 15

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 1093

ER -

ID: 129788543