Condensed tannins act against cattle nematodes

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Condensed tannins act against cattle nematodes. / Novobilský, Adam; Mueller-Harvey, Irene; Thamsborg, Stig Milan.

I: Journal of Veterinary Parasitology, Bind 182, Nr. 2-4, 2011, s. 213-220.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Novobilský, A, Mueller-Harvey, I & Thamsborg, SM 2011, 'Condensed tannins act against cattle nematodes', Journal of Veterinary Parasitology, bind 182, nr. 2-4, s. 213-220. https://doi.org/10.1016/j.vetpar.2011.06.003

APA

Novobilský, A., Mueller-Harvey, I., & Thamsborg, S. M. (2011). Condensed tannins act against cattle nematodes. Journal of Veterinary Parasitology, 182(2-4), 213-220. https://doi.org/10.1016/j.vetpar.2011.06.003

Vancouver

Novobilský A, Mueller-Harvey I, Thamsborg SM. Condensed tannins act against cattle nematodes. Journal of Veterinary Parasitology. 2011;182(2-4):213-220. https://doi.org/10.1016/j.vetpar.2011.06.003

Author

Novobilský, Adam ; Mueller-Harvey, Irene ; Thamsborg, Stig Milan. / Condensed tannins act against cattle nematodes. I: Journal of Veterinary Parasitology. 2011 ; Bind 182, Nr. 2-4. s. 213-220.

Bibtex

@article{4858828ba5c94a54bde85584a9217319,
title = "Condensed tannins act against cattle nematodes",
abstract = "The use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (GIN) in ruminants. Direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat GIN. These anthelmintic properties are mainly associated with condensed tannins. In the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine GIN. Effects of Onobrychis viciifolia, Lotus pedunculatus and Lotus corniculatus condensed tannin (CT) extracts on Cooperia oncophora and Ostertagia ostertagi were determined by a larval feeding inhibition assay (LFIA) and a larval exsheathment assay (LEA). In the LFIA, all three plant extracts significantly inhibited larval feeding behaviour of both C. oncophora and O. ostertagi first stage larvae in a dose-dependent manner. The L. pedunculatus extract, based on EC(50) (effective concentration for 50% inhibition), was the most effective against both nematodes, followed by O. viciifolia and L. corniculatus. The effect of CT extracts upon larval feeding behaviour correlates with CT content and procyanidin/prodelphidin ratio. Larval exsheathment of C. oncophora and O. ostertagi L3 larvae (third stage larvae) was also affected by CT extracts from all three plants. In both in vitro assays, extracts with added polyvinylpolypyrrolidone, an inhibitor of tannins, generated almost the same values as the negative control; this confirms the role of CT in the anthelmintic effect of these plant extracts. Our results, therefore, indicated that tannin-containing plants could act against cattle nematodes.",
keywords = "Former LIFE faculty, Condensed tannins, Cattle, Nematodes, Ostertagia ostertagi, in vitro assay, Exsheathment, Larval feeding inhibition assay",
author = "Adam Novobilsk{\'y} and Irene Mueller-Harvey and Thamsborg, {Stig Milan}",
note = "Copyright {\textcopyright} 2011 Elsevier B.V. All rights reserved.",
year = "2011",
doi = "10.1016/j.vetpar.2011.06.003",
language = "English",
volume = "182",
pages = "213--220",
journal = "Journal of Veterinary Parasitology",
issn = "0971-1031",
publisher = "Indian Association for the Advancement of Veterinary Parasitology",
number = "2-4",

}

RIS

TY - JOUR

T1 - Condensed tannins act against cattle nematodes

AU - Novobilský, Adam

AU - Mueller-Harvey, Irene

AU - Thamsborg, Stig Milan

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011

Y1 - 2011

N2 - The use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (GIN) in ruminants. Direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat GIN. These anthelmintic properties are mainly associated with condensed tannins. In the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine GIN. Effects of Onobrychis viciifolia, Lotus pedunculatus and Lotus corniculatus condensed tannin (CT) extracts on Cooperia oncophora and Ostertagia ostertagi were determined by a larval feeding inhibition assay (LFIA) and a larval exsheathment assay (LEA). In the LFIA, all three plant extracts significantly inhibited larval feeding behaviour of both C. oncophora and O. ostertagi first stage larvae in a dose-dependent manner. The L. pedunculatus extract, based on EC(50) (effective concentration for 50% inhibition), was the most effective against both nematodes, followed by O. viciifolia and L. corniculatus. The effect of CT extracts upon larval feeding behaviour correlates with CT content and procyanidin/prodelphidin ratio. Larval exsheathment of C. oncophora and O. ostertagi L3 larvae (third stage larvae) was also affected by CT extracts from all three plants. In both in vitro assays, extracts with added polyvinylpolypyrrolidone, an inhibitor of tannins, generated almost the same values as the negative control; this confirms the role of CT in the anthelmintic effect of these plant extracts. Our results, therefore, indicated that tannin-containing plants could act against cattle nematodes.

AB - The use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (GIN) in ruminants. Direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat GIN. These anthelmintic properties are mainly associated with condensed tannins. In the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine GIN. Effects of Onobrychis viciifolia, Lotus pedunculatus and Lotus corniculatus condensed tannin (CT) extracts on Cooperia oncophora and Ostertagia ostertagi were determined by a larval feeding inhibition assay (LFIA) and a larval exsheathment assay (LEA). In the LFIA, all three plant extracts significantly inhibited larval feeding behaviour of both C. oncophora and O. ostertagi first stage larvae in a dose-dependent manner. The L. pedunculatus extract, based on EC(50) (effective concentration for 50% inhibition), was the most effective against both nematodes, followed by O. viciifolia and L. corniculatus. The effect of CT extracts upon larval feeding behaviour correlates with CT content and procyanidin/prodelphidin ratio. Larval exsheathment of C. oncophora and O. ostertagi L3 larvae (third stage larvae) was also affected by CT extracts from all three plants. In both in vitro assays, extracts with added polyvinylpolypyrrolidone, an inhibitor of tannins, generated almost the same values as the negative control; this confirms the role of CT in the anthelmintic effect of these plant extracts. Our results, therefore, indicated that tannin-containing plants could act against cattle nematodes.

KW - Former LIFE faculty

KW - Condensed tannins

KW - Cattle

KW - Nematodes

KW - Ostertagia ostertagi

KW - in vitro assay

KW - Exsheathment

KW - Larval feeding inhibition assay

U2 - 10.1016/j.vetpar.2011.06.003

DO - 10.1016/j.vetpar.2011.06.003

M3 - Journal article

C2 - 21726942

VL - 182

SP - 213

EP - 220

JO - Journal of Veterinary Parasitology

JF - Journal of Veterinary Parasitology

SN - 0971-1031

IS - 2-4

ER -

ID: 36090956