Danish apple cultivars: genetic diversity, parentage and breeding potential

Publikation: Bidrag til bog/antologi/rapportKonferencebidrag i proceedingsForskningfagfællebedømt

Abstract
We made a genetic analysis of about 500 apple cultivars, which either originate
from Denmark or have been of greater importance in Denmark. To study the diversity and parentage among the cultivars, 15 SSR markers were used. A new PCR-based protocol for identification of S-alleles was developed and used to study the S-alleles among cultivars. The protocol using general S-locus primers and digestion with two restriction enzymes, for fragment analysis on an automated DNA sequencer fastens the process and gives an accurate estimation of fragment lengths. The ploidy level was determined using flow-cytometry. All cultivars were distinguishable by the SSRmarkers though Danish originating cultivars did not form a group distinct from foreign cultivars. The parentage of several well-known cultivars was revealed.
OriginalsprogEngelsk
TitelProceedings of the XIV EUCARPIA Symposium on Fruit Breeding and Genetics
Antal sider4
ForlagInternational Society for Horticultural Science
Publikationsdato2017
Sider355-358
DOI
StatusUdgivet - 2017
BegivenhedEucarpia Symposium on Fruit Breeding and Genetics 2015 - Bologna, Italien
Varighed: 14 jun. 201518 jun. 2015
Konferencens nummer: 14
http://www.ishs.org/symposium/382

Konference

KonferenceEucarpia Symposium on Fruit Breeding and Genetics 2015
Nummer14
LandItalien
ByBologna
Periode14/06/201518/06/2015
Internetadresse
NavnActa Horticulturae
Vol/bind1172
ISSN0567-7572

    Forskningsområder

  • Æbler - Genetisk diversitet, Forædlingspotentiale

ID: 186529046