Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces. / Sindberg, Diana; Nissen, Sofie; Anh, Nguyen Thi Lan; Johansen, Maria Vang.

I: Veterinary Parasitology, Bind 195, Nr. 1-2, 2013, s. 192-197.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Sindberg, D, Nissen, S, Anh, NTL & Johansen, MV 2013, 'Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces', Veterinary Parasitology, bind 195, nr. 1-2, s. 192-197. https://doi.org/10.1016/j.vetpar.2013.03.016

APA

Sindberg, D., Nissen, S., Anh, N. T. L., & Johansen, M. V. (2013). Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces. Veterinary Parasitology, 195(1-2), 192-197. https://doi.org/10.1016/j.vetpar.2013.03.016

Vancouver

Sindberg D, Nissen S, Anh NTL, Johansen MV. Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces. Veterinary Parasitology. 2013;195(1-2):192-197. https://doi.org/10.1016/j.vetpar.2013.03.016

Author

Sindberg, Diana ; Nissen, Sofie ; Anh, Nguyen Thi Lan ; Johansen, Maria Vang. / Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces. I: Veterinary Parasitology. 2013 ; Bind 195, Nr. 1-2. s. 192-197.

Bibtex

@article{570fa301fb79444aa13b1b56cda464f2,
title = "Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces",
abstract = "The study was conducted to evaluate copro-diagnostic techniques for detection of small trematode eggs in dogs. FLOTAC, a novel flotation technique, and DBL, a sieving and sedimentation technique developed at the former Danish Bilharziasis Laboratory (DBL), were compared using 53 subsamples from four copro-positive dogs. Moreover, a modified version of the DBL technique and the Kato-Katz (KK) thick smear were later compared using faecal samples from 21 dogs. The four techniques were pair-wise compared regarding sensitivity, infection intensity and practical applicability. For the former two techniques, egg recovery subsequent to storage and reproducibility were also compared. The DBL technique detected all 53 subsamples positive for small trematode eggs. Based on 17 subsamples, mean infection intensity of 47±49 eggs per gram of faeces (EPG) was detected by the technique. Due to large amount of sediment, examination of a single subsample required an average of 3hours. The FLOTAC technique was found less sensitive (82%) than the DBL technique and recovered significantly less eggs (4±6 EPG). Both sensitivity and intensity were further reduced following storage. As the FLOTAC technique requires specialised equipment, safety disposal and personal protective equipment, it was found less suited than the DBL technique for a basic laboratory. Additionally, poor reproducibilities were found for both the DBL and FLOTAC techniques (30±15% and 38±33%, respectively). Based on the 21 faecal samples, the modified version of the DBL technique was found more sensitive (85%) than the KK technique (68%), whereas egg counts were significantly higher for the latter (23±26 EPG vs. 482±909 EPG). By modifying the DBL technique, it was possible to diminish the retained sediment and examination time to a maximum of an hour, which was also the time required by the KK technique, although the latter was faster and more easily processed. Based on the results obtained in this study, none of the techniques evaluated were found applicable in their current form for detection of small trematode eggs in faeces from dogs in Vietnam.",
author = "Diana Sindberg and Sofie Nissen and Anh, {Nguyen Thi Lan} and Johansen, {Maria Vang}",
note = "Copyright {\textcopyright} 2013 Elsevier B.V. All rights reserved.",
year = "2013",
doi = "10.1016/j.vetpar.2013.03.016",
language = "English",
volume = "195",
pages = "192--197",
journal = "Veterinary Parasitology",
issn = "0304-4017",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Evaluation of four coproscopic techniques for detection of small trematode eggs in dog faeces

AU - Sindberg, Diana

AU - Nissen, Sofie

AU - Anh, Nguyen Thi Lan

AU - Johansen, Maria Vang

N1 - Copyright © 2013 Elsevier B.V. All rights reserved.

PY - 2013

Y1 - 2013

N2 - The study was conducted to evaluate copro-diagnostic techniques for detection of small trematode eggs in dogs. FLOTAC, a novel flotation technique, and DBL, a sieving and sedimentation technique developed at the former Danish Bilharziasis Laboratory (DBL), were compared using 53 subsamples from four copro-positive dogs. Moreover, a modified version of the DBL technique and the Kato-Katz (KK) thick smear were later compared using faecal samples from 21 dogs. The four techniques were pair-wise compared regarding sensitivity, infection intensity and practical applicability. For the former two techniques, egg recovery subsequent to storage and reproducibility were also compared. The DBL technique detected all 53 subsamples positive for small trematode eggs. Based on 17 subsamples, mean infection intensity of 47±49 eggs per gram of faeces (EPG) was detected by the technique. Due to large amount of sediment, examination of a single subsample required an average of 3hours. The FLOTAC technique was found less sensitive (82%) than the DBL technique and recovered significantly less eggs (4±6 EPG). Both sensitivity and intensity were further reduced following storage. As the FLOTAC technique requires specialised equipment, safety disposal and personal protective equipment, it was found less suited than the DBL technique for a basic laboratory. Additionally, poor reproducibilities were found for both the DBL and FLOTAC techniques (30±15% and 38±33%, respectively). Based on the 21 faecal samples, the modified version of the DBL technique was found more sensitive (85%) than the KK technique (68%), whereas egg counts were significantly higher for the latter (23±26 EPG vs. 482±909 EPG). By modifying the DBL technique, it was possible to diminish the retained sediment and examination time to a maximum of an hour, which was also the time required by the KK technique, although the latter was faster and more easily processed. Based on the results obtained in this study, none of the techniques evaluated were found applicable in their current form for detection of small trematode eggs in faeces from dogs in Vietnam.

AB - The study was conducted to evaluate copro-diagnostic techniques for detection of small trematode eggs in dogs. FLOTAC, a novel flotation technique, and DBL, a sieving and sedimentation technique developed at the former Danish Bilharziasis Laboratory (DBL), were compared using 53 subsamples from four copro-positive dogs. Moreover, a modified version of the DBL technique and the Kato-Katz (KK) thick smear were later compared using faecal samples from 21 dogs. The four techniques were pair-wise compared regarding sensitivity, infection intensity and practical applicability. For the former two techniques, egg recovery subsequent to storage and reproducibility were also compared. The DBL technique detected all 53 subsamples positive for small trematode eggs. Based on 17 subsamples, mean infection intensity of 47±49 eggs per gram of faeces (EPG) was detected by the technique. Due to large amount of sediment, examination of a single subsample required an average of 3hours. The FLOTAC technique was found less sensitive (82%) than the DBL technique and recovered significantly less eggs (4±6 EPG). Both sensitivity and intensity were further reduced following storage. As the FLOTAC technique requires specialised equipment, safety disposal and personal protective equipment, it was found less suited than the DBL technique for a basic laboratory. Additionally, poor reproducibilities were found for both the DBL and FLOTAC techniques (30±15% and 38±33%, respectively). Based on the 21 faecal samples, the modified version of the DBL technique was found more sensitive (85%) than the KK technique (68%), whereas egg counts were significantly higher for the latter (23±26 EPG vs. 482±909 EPG). By modifying the DBL technique, it was possible to diminish the retained sediment and examination time to a maximum of an hour, which was also the time required by the KK technique, although the latter was faster and more easily processed. Based on the results obtained in this study, none of the techniques evaluated were found applicable in their current form for detection of small trematode eggs in faeces from dogs in Vietnam.

U2 - 10.1016/j.vetpar.2013.03.016

DO - 10.1016/j.vetpar.2013.03.016

M3 - Journal article

C2 - 23582664

VL - 195

SP - 192

EP - 197

JO - Veterinary Parasitology

JF - Veterinary Parasitology

SN - 0304-4017

IS - 1-2

ER -

ID: 46904450