Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice

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Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice. / Hufeldt, Majbritt Ravn; Nielsen, Dennis Sandris; Vogensen, Finn Kvist; Midtvedt, T.; Hansen, Axel Jacob Kornerup.

I: Laboratory Animals. Journal of the Laboratory Animal Science Association, Bind 44, Nr. 4, 2010, s. 283-289.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Hufeldt, MR, Nielsen, DS, Vogensen, FK, Midtvedt, T & Hansen, AJK 2010, 'Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice', Laboratory Animals. Journal of the Laboratory Animal Science Association, bind 44, nr. 4, s. 283-289. https://doi.org/10.1258/la.2010.010058

APA

Hufeldt, M. R., Nielsen, D. S., Vogensen, F. K., Midtvedt, T., & Hansen, A. J. K. (2010). Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice. Laboratory Animals. Journal of the Laboratory Animal Science Association, 44(4), 283-289. https://doi.org/10.1258/la.2010.010058

Vancouver

Hufeldt MR, Nielsen DS, Vogensen FK, Midtvedt T, Hansen AJK. Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice. Laboratory Animals. Journal of the Laboratory Animal Science Association. 2010;44(4):283-289. https://doi.org/10.1258/la.2010.010058

Author

Hufeldt, Majbritt Ravn ; Nielsen, Dennis Sandris ; Vogensen, Finn Kvist ; Midtvedt, T. ; Hansen, Axel Jacob Kornerup. / Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice. I: Laboratory Animals. Journal of the Laboratory Animal Science Association. 2010 ; Bind 44, Nr. 4. s. 283-289.

Bibtex

@article{1b6a06a733134100b6ec834e940ad4b7,
title = "Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice",
abstract = "The gut microbiota (GM) may influence disease expression in several animal models for inflammatory diseases. It may therefore seem reasonable to pursue reduction in the number of animals used for individual studies by reducing the variation in the GM. Previous studies have shown that the composition of the GM is related to genetics to a certain extent. We hypothesized that the GM similarity in a group of mice born by mothers not being sisters would be lower than that in a group born by mothers being sisters. The lower similarity could lead to clustering of the GM of mice born by non-sisters according to their mothers, while such clustering would not be visible if the mothers were sisters. We used 16S rRNA gene (V3 region) polymerase chain reaction-derived amplicon profiling by denaturing gradient gel electrophoresis (DGGE) to study the GM composition in caecum samples of 33 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were sisters, as well as caecum samples of 35 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were not sisters. Principal component analysis revealed a significant difference between the litters from the breeding set-up with dam breeders that were not sisters, whereas no significant difference between the litters based on the breeding set-up with dam breeders that were sisters was observed. The results obtained indicate that the systematic variation in the GM of inbred mice can be reduced by increasing the family relatedness of the breeding pairs.",
keywords = "Former LIFE faculty, Laboratory animals, variation of the gut microbiota, Reduction, PCR/DGGE",
author = "Hufeldt, {Majbritt Ravn} and Nielsen, {Dennis Sandris} and Vogensen, {Finn Kvist} and T. Midtvedt and Hansen, {Axel Jacob Kornerup}",
year = "2010",
doi = "10.1258/la.2010.010058",
language = "English",
volume = "44",
pages = "283--289",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "SAGE Publications",
number = "4",

}

RIS

TY - JOUR

T1 - Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice

AU - Hufeldt, Majbritt Ravn

AU - Nielsen, Dennis Sandris

AU - Vogensen, Finn Kvist

AU - Midtvedt, T.

AU - Hansen, Axel Jacob Kornerup

PY - 2010

Y1 - 2010

N2 - The gut microbiota (GM) may influence disease expression in several animal models for inflammatory diseases. It may therefore seem reasonable to pursue reduction in the number of animals used for individual studies by reducing the variation in the GM. Previous studies have shown that the composition of the GM is related to genetics to a certain extent. We hypothesized that the GM similarity in a group of mice born by mothers not being sisters would be lower than that in a group born by mothers being sisters. The lower similarity could lead to clustering of the GM of mice born by non-sisters according to their mothers, while such clustering would not be visible if the mothers were sisters. We used 16S rRNA gene (V3 region) polymerase chain reaction-derived amplicon profiling by denaturing gradient gel electrophoresis (DGGE) to study the GM composition in caecum samples of 33 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were sisters, as well as caecum samples of 35 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were not sisters. Principal component analysis revealed a significant difference between the litters from the breeding set-up with dam breeders that were not sisters, whereas no significant difference between the litters based on the breeding set-up with dam breeders that were sisters was observed. The results obtained indicate that the systematic variation in the GM of inbred mice can be reduced by increasing the family relatedness of the breeding pairs.

AB - The gut microbiota (GM) may influence disease expression in several animal models for inflammatory diseases. It may therefore seem reasonable to pursue reduction in the number of animals used for individual studies by reducing the variation in the GM. Previous studies have shown that the composition of the GM is related to genetics to a certain extent. We hypothesized that the GM similarity in a group of mice born by mothers not being sisters would be lower than that in a group born by mothers being sisters. The lower similarity could lead to clustering of the GM of mice born by non-sisters according to their mothers, while such clustering would not be visible if the mothers were sisters. We used 16S rRNA gene (V3 region) polymerase chain reaction-derived amplicon profiling by denaturing gradient gel electrophoresis (DGGE) to study the GM composition in caecum samples of 33 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were sisters, as well as caecum samples of 35 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were not sisters. Principal component analysis revealed a significant difference between the litters from the breeding set-up with dam breeders that were not sisters, whereas no significant difference between the litters based on the breeding set-up with dam breeders that were sisters was observed. The results obtained indicate that the systematic variation in the GM of inbred mice can be reduced by increasing the family relatedness of the breeding pairs.

KW - Former LIFE faculty

KW - Laboratory animals

KW - variation of the gut microbiota

KW - Reduction

KW - PCR/DGGE

U2 - 10.1258/la.2010.010058

DO - 10.1258/la.2010.010058

M3 - Journal article

C2 - 20713427

VL - 44

SP - 283

EP - 289

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

IS - 4

ER -

ID: 32375174