Identification of cross-reacting T-cell epitopes in structural and non-structural proteins of swine and pandemic H1N1 influenza a virus strains in pigs
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Heterologous protection against swine influenza viruses (SwIVs) of different lineages is an important concern for the pig industry. Cross-protection between ‘avian-like’ H1N1 and 2009 pandemic H1N1 lineages has been observed previously, indicating the involvement of cross-reacting T-cells. Here, reverse vaccinology was applied to identify cross-reacting MHC class I T-cell epitopes from two different SwIV H1 lineages in pigs. In silico prediction followed by in vitro and in vivo testing was used to identify SLA-1*0702 T-cell epitopes in heterologous SwIV-infected pigs. Following viral infection, tetramer specific T-cell populations were identified. The majority of the identified T-cell epitopes were conserved between the examined lineages, suggesting that targeting cross-reactive T-cell epitopes could be used to improve vaccines against SwIV in SLA-1*0702-positive pigs.
Originalsprog | Engelsk |
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Artikelnummer | 000748 |
Tidsskrift | Journal of General Virology |
Vol/bind | 98 |
Udgave nummer | 5 |
Sider (fra-til) | 895-899 |
Antal sider | 5 |
ISSN | 0022-1317 |
DOI | |
Status | Udgivet - 2017 |
Eksternt udgivet | Ja |
ID: 247394601