Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs

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Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs. / Sun, Jing; Li, Yanqi; Nguyen, Duc Ninh; Mortensen, Martin Steen; van den Akker, Chris H. P.; Skeath, Tom; Pors, Susanne Elisabeth; Pankratova, Stanislava; Rudloff, Silvia; Sørensen, Søren Johannes; Burrin, Douglas G.; Thymann, Thomas; Sangild, Per Torp.

I: Journal of Nutrition, Bind 148, Nr. 3, 2018, s. 336-347.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Sun, J, Li, Y, Nguyen, DN, Mortensen, MS, van den Akker, CHP, Skeath, T, Pors, SE, Pankratova, S, Rudloff, S, Sørensen, SJ, Burrin, DG, Thymann, T & Sangild, PT 2018, 'Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs', Journal of Nutrition, bind 148, nr. 3, s. 336-347. https://doi.org/10.1093/jn/nxx033

APA

Sun, J., Li, Y., Nguyen, D. N., Mortensen, M. S., van den Akker, C. H. P., Skeath, T., Pors, S. E., Pankratova, S., Rudloff, S., Sørensen, S. J., Burrin, D. G., Thymann, T., & Sangild, P. T. (2018). Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs. Journal of Nutrition, 148(3), 336-347. https://doi.org/10.1093/jn/nxx033

Vancouver

Sun J, Li Y, Nguyen DN, Mortensen MS, van den Akker CHP, Skeath T o.a. Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs. Journal of Nutrition. 2018;148(3):336-347. https://doi.org/10.1093/jn/nxx033

Author

Sun, Jing ; Li, Yanqi ; Nguyen, Duc Ninh ; Mortensen, Martin Steen ; van den Akker, Chris H. P. ; Skeath, Tom ; Pors, Susanne Elisabeth ; Pankratova, Stanislava ; Rudloff, Silvia ; Sørensen, Søren Johannes ; Burrin, Douglas G. ; Thymann, Thomas ; Sangild, Per Torp. / Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs. I: Journal of Nutrition. 2018 ; Bind 148, Nr. 3. s. 336-347.

Bibtex

@article{3a8fa8d950fc497ea71cc0b276d7bacc,
title = "Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs",
abstract = "Background: Nutrient fortification of human milk is often required to secure adequate growth and organ development for very preterm infants. There is concern that formula-based fortifiers (FFs) induce intestinal dysfunction, feeding intolerance, and necrotizing enterocolitis (NEC). Bovine colostrum (BC) may be an alternative nutrient fortifier, considering its high content of protein and milk bioactive factors.Objective: We investigated whether BC was superior to an FF product based on processed bovine milk and vegetable oil to fortify donor human milk (DHM) for preterm pigs, used as a model for infants.Methods: Sixty preterm pigs from 4 sows (Danish Landrace × Large White × Duroc, birth weight 944 ± 29 g) received decreasing volumes of parenteral nutrition (96-72 mL ⋅ kg-1 ⋅ d-1) and increasing volumes of enteral nutrition (24-132 mL ⋅ kg-1 ⋅ d-1) for 8 d. Pigs were fed donor porcine milk (DPM) and DHM with or without FF or BC fortification (+4.6 g protein ⋅ kg-1 ⋅ d-1).Results: DPM-fed pigs showed higher growth (10-fold), protein synthesis (+15-30%), villus heights, lactase and peptidase activities (+30%), and reduced intestinal cytokines (-50%) relative to DHM pigs (all P < 0.05). Fortification increased protein synthesis (+20-30%), but with higher weight gain and lower urea and cortisol concentrations for DHM+BC compared with DHM+FF pigs (2- to 3-fold differences, all P ≤ 0.06). DHM+FF pigs showed more diarrhea and reduced lactase and peptidase activities, hexose uptake, and villus heights relative to DHM+BC or DHM pigs (30-90% differences, P < 0.05). Fortification did not affect NEC incidence but DHM+BC pigs had lower colonic interleukin (IL)-6 and IL-8 concentrations relative to the remaining pigs (-30%, P = 0.06). DHM+FF pigs had higher stomach bacterial load than did DHM, and higher bacterial density along intestinal villi than did DHM and DHM+BC pigs (2- to 3-fold, P < 0.05).Conclusions: The FF product investigated in this study reduced growth, intestinal function, and protein utilization in DHM-fed preterm pigs, relative to BC as fortifier. The relevance of BC as an alternative nutrient fortifier for preterm infants should be tested.",
author = "Jing Sun and Yanqi Li and Nguyen, {Duc Ninh} and Mortensen, {Martin Steen} and {van den Akker}, {Chris H. P.} and Tom Skeath and Pors, {Susanne Elisabeth} and Stanislava Pankratova and Silvia Rudloff and S{\o}rensen, {S{\o}ren Johannes} and Burrin, {Douglas G.} and Thomas Thymann and Sangild, {Per Torp}",
year = "2018",
doi = "10.1093/jn/nxx033",
language = "English",
volume = "148",
pages = "336--347",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "3",

}

RIS

TY - JOUR

T1 - Nutrient Fortification of Human Donor Milk Affects Intestinal Function and Protein Metabolism in Preterm Pigs

AU - Sun, Jing

AU - Li, Yanqi

AU - Nguyen, Duc Ninh

AU - Mortensen, Martin Steen

AU - van den Akker, Chris H. P.

AU - Skeath, Tom

AU - Pors, Susanne Elisabeth

AU - Pankratova, Stanislava

AU - Rudloff, Silvia

AU - Sørensen, Søren Johannes

AU - Burrin, Douglas G.

AU - Thymann, Thomas

AU - Sangild, Per Torp

PY - 2018

Y1 - 2018

N2 - Background: Nutrient fortification of human milk is often required to secure adequate growth and organ development for very preterm infants. There is concern that formula-based fortifiers (FFs) induce intestinal dysfunction, feeding intolerance, and necrotizing enterocolitis (NEC). Bovine colostrum (BC) may be an alternative nutrient fortifier, considering its high content of protein and milk bioactive factors.Objective: We investigated whether BC was superior to an FF product based on processed bovine milk and vegetable oil to fortify donor human milk (DHM) for preterm pigs, used as a model for infants.Methods: Sixty preterm pigs from 4 sows (Danish Landrace × Large White × Duroc, birth weight 944 ± 29 g) received decreasing volumes of parenteral nutrition (96-72 mL ⋅ kg-1 ⋅ d-1) and increasing volumes of enteral nutrition (24-132 mL ⋅ kg-1 ⋅ d-1) for 8 d. Pigs were fed donor porcine milk (DPM) and DHM with or without FF or BC fortification (+4.6 g protein ⋅ kg-1 ⋅ d-1).Results: DPM-fed pigs showed higher growth (10-fold), protein synthesis (+15-30%), villus heights, lactase and peptidase activities (+30%), and reduced intestinal cytokines (-50%) relative to DHM pigs (all P < 0.05). Fortification increased protein synthesis (+20-30%), but with higher weight gain and lower urea and cortisol concentrations for DHM+BC compared with DHM+FF pigs (2- to 3-fold differences, all P ≤ 0.06). DHM+FF pigs showed more diarrhea and reduced lactase and peptidase activities, hexose uptake, and villus heights relative to DHM+BC or DHM pigs (30-90% differences, P < 0.05). Fortification did not affect NEC incidence but DHM+BC pigs had lower colonic interleukin (IL)-6 and IL-8 concentrations relative to the remaining pigs (-30%, P = 0.06). DHM+FF pigs had higher stomach bacterial load than did DHM, and higher bacterial density along intestinal villi than did DHM and DHM+BC pigs (2- to 3-fold, P < 0.05).Conclusions: The FF product investigated in this study reduced growth, intestinal function, and protein utilization in DHM-fed preterm pigs, relative to BC as fortifier. The relevance of BC as an alternative nutrient fortifier for preterm infants should be tested.

AB - Background: Nutrient fortification of human milk is often required to secure adequate growth and organ development for very preterm infants. There is concern that formula-based fortifiers (FFs) induce intestinal dysfunction, feeding intolerance, and necrotizing enterocolitis (NEC). Bovine colostrum (BC) may be an alternative nutrient fortifier, considering its high content of protein and milk bioactive factors.Objective: We investigated whether BC was superior to an FF product based on processed bovine milk and vegetable oil to fortify donor human milk (DHM) for preterm pigs, used as a model for infants.Methods: Sixty preterm pigs from 4 sows (Danish Landrace × Large White × Duroc, birth weight 944 ± 29 g) received decreasing volumes of parenteral nutrition (96-72 mL ⋅ kg-1 ⋅ d-1) and increasing volumes of enteral nutrition (24-132 mL ⋅ kg-1 ⋅ d-1) for 8 d. Pigs were fed donor porcine milk (DPM) and DHM with or without FF or BC fortification (+4.6 g protein ⋅ kg-1 ⋅ d-1).Results: DPM-fed pigs showed higher growth (10-fold), protein synthesis (+15-30%), villus heights, lactase and peptidase activities (+30%), and reduced intestinal cytokines (-50%) relative to DHM pigs (all P < 0.05). Fortification increased protein synthesis (+20-30%), but with higher weight gain and lower urea and cortisol concentrations for DHM+BC compared with DHM+FF pigs (2- to 3-fold differences, all P ≤ 0.06). DHM+FF pigs showed more diarrhea and reduced lactase and peptidase activities, hexose uptake, and villus heights relative to DHM+BC or DHM pigs (30-90% differences, P < 0.05). Fortification did not affect NEC incidence but DHM+BC pigs had lower colonic interleukin (IL)-6 and IL-8 concentrations relative to the remaining pigs (-30%, P = 0.06). DHM+FF pigs had higher stomach bacterial load than did DHM, and higher bacterial density along intestinal villi than did DHM and DHM+BC pigs (2- to 3-fold, P < 0.05).Conclusions: The FF product investigated in this study reduced growth, intestinal function, and protein utilization in DHM-fed preterm pigs, relative to BC as fortifier. The relevance of BC as an alternative nutrient fortifier for preterm infants should be tested.

U2 - 10.1093/jn/nxx033

DO - 10.1093/jn/nxx033

M3 - Journal article

C2 - 29462356

VL - 148

SP - 336

EP - 347

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 3

ER -

ID: 192447597