Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

  • Cathrine Bie Petersen
  • Ann-Britt Nygaard Hillig
  • Birgitte Viuff
  • Fredholm, Merete
  • Bent Aasted
  • Jan Salomonsen

Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.

OriginalsprogEngelsk
TidsskriftDevelopmental & Comparative Immunology
Vol/bind31
Udgave nummer6
Sider (fra-til)618-631
Antal sider14
ISSN0145-305X
DOI
StatusUdgivet - 2007

ID: 8043334