The interaction of antimicrobial peptides with the membrane and intracellular targets of Staphylococcus aureus investigated by ATP leakage, DNA-binding analysis, and the expression of a LexA-controlled gene, recA

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.

OriginalsprogEngelsk
TitelAntimicrobial Peptides
Antal sider9
Vol/bind1548
ForlagHumana Press
Publikationsdato2017
Sider297-305
ISBN (Trykt)978-1-4939-6735-3
ISBN (Elektronisk)978-1-4939-6737-7
DOI
StatusUdgivet - 2017
NavnMethods in Molecular Biology
Vol/bind1548
ISSN1064-3745

ID: 188416444