This review documents some new advances in the field of Veterinary medicine with specific focus on the exciting research area of myo-fascia. It presents some of the latest findings in structure as well as function of these interconnected tissues, as well as highlighting the effects and benefits of treatment for such underlying issues as regional stiffness affecting performance, and the role of myofascial kinetic lines. It likewise addresses some of the new and non-invasive techniques such as accelerometry, multi-frequency bioimpedance and acoustic myography that have emerged over recent years, and illustrates how they can be readily adopted in the Veterinary clinic to assess muscle imbalance and injury as well as to direct such treatment as myofascial release therapy, acupuncture and proprioception and to follow rehabilitation.