Improved diagnostics

Rapid and accurate identification and antimicrobial susceptibility testing of bacterial pathogens is of key importance for guidance of antimicrobial choice and best management of bacterial diseases at the patient and herd level. We pursue the objective of improving diagnosis of bacterial infections in both production and companion animals using a comprehensive approach ranging from optimization of diagnostic and antimicrobial susceptibility testing methods and procedures [1-3] to development of new Point-of-Care tests [4] and clinical breakpoints [5].

These are the current projects in this area:

Optimizing antimicrobial susceptibility testing and treatment of mink – a PK/PD approach

Objective: to produce pharmacokinetic (PK) data for antimicrobials used in mink production, and to use these data in mathematical modelling to create (i) specific dosage regimens targeting the most common mink pathogens, and (ii) clinical breakpoints for antimicrobial susceptibility testing of mink pathogens.
PI: Peter Damborg
Scientist is charge: Amir Atabak Ronaghinia
Key collaborators: Tina Struve, Pierre-Louis Toutain, Karl Pedersen
Funding: Innovation Fund Denmark and the Danish Fur Breeder’s Association
Duration: 2017 - 2021
Budget: 2,500,000 DKK

LAMP point-of-care testing to guide antimicrobial choice for treatment of canine pyoderma

Objective: To develop a point-of-care (PoC) based on loop-mediated isothermal amplification (LAMP) for rapid and inexpensive detection of Staphylococcus pseudintermedius strains resistant to the first line antimicrobials recommended for systemic therapy of pyoderma in dogs (clindamycin, cephalexin, amoxicillin clavulanate)
PI: Luca Guardabassi (coordinator)
Scientist is charge: Manja Ipsen Hanegård
Key collaborators: Mette Schjærff, Anette Loeffler (UK), Roberto La Ragione (UK)
Funding: ANICURA
Duration: 2020 – 2021
Budget: 150,000 DKK

Active surveillance of antimicrobial resistance in pigs

Objective: To investigate antimicrobial resistance in enterotoxigenic Escherichia coli (ETEC) in Danish pigs, and to develop a point-of-care diagnostic test for rapid detection and antimicrobial susceptibility testing of ETEC in pigs with post-weaning diarrhea.
PI: Peter Damborg
Scientist is charge: Prabha Subramani
Key collaborators: Luca Guardabassi, Ken Steen Pedersen, Svend Haugegaard
Funding: the Danish Veterinary and Food Administration (Fødevarestyrelsen)
Duration: 2020 – 2022
Budget: 2,000,000 DKK

Key references:

  1. Toutain PL, Bousquet-Mélou A, Damborg P, Ferran AA, Mevius D, Pelligand L, Veldman KT, Lees P. 2017. En Route towards European Clinical Breakpoints for Veterinary Antimicrobial Susceptibility Testing: A Position Paper Explaining the VetCAST Approach. Front Microbiol 8:2344.
  2. Guardabassi L, Hedberg S, Jessen LR, Damborg P. 2015. Optimization and evaluation of Flexicult® Vet for detection, identification and antimicrobial susceptibility testing of bacterial uropathogens in small animal veterinary practice. Acta Vet Scand 57:72.
  3. Larsen RF, Boysen L, Jessen LR, Guardabassi L, Damborg P. 2018. Diversity of Staphylococcus pseudintermedius in carriage sites and skin lesions of dogs with superficial bacterial folliculitis: potential implications for diagnostic testing and therapy. Vet Dermatol. [Epub ahead of print]
  4. Moberg FS, Langhorn R, Bertelsen PV, Pilegaard LM, Sørensen TM, Bjørnvad CR, Damborg P, Kieler IN, Jessen LR. 2019. Subclinical bacteriuria in a mixed population of 179 middle-aged and elderly cats: a prospective cross-sectional study. J Feline Med Surg Sep 20:1098612X19874141.
  5. Sørensen TM, Jensen AB, Damborg P, Bjørnvad CR, Guardabassi L, Jessen LR. 2016. Evaluation of different sampling methods and criteria for diagnosing canine urinary tract infection by quantitative bacterial culture. Vet J 216:168-73.
  6. Sørensen TM, Bjørnvad CR, Cordoba G, Damborg P, Guardabassi L, Siersma V, Bjerrum L, Jessen LR. 2018. Effects of Diagnostic Work-Up on Medical Decision-Making for Canine Urinary Tract Infection: An Observational Study in Danish Small Animal Practices. J Vet Intern Med 32:743-751.
  7. Maaland MG, Papich MG, Turnidge J, Guardabassi L. 2013. Pharmacodynamics of doxycycline and tetracycline against Staphylococcus pseudintermedius: proposal of canine-specific breakpoints for doxycycline. J Clin Microbiol 51:3547-54.