Identification of novel vaccine targets against G. anatis and A. pleuropneumoniae

The aim of our vaccine projects is to develop vaccine prototypes that offer protection in a serotype-independent manner.

Gallibacterium anatis
G. anatis is an opportunistic pathogen that cause of salpingitis and peritonitis in egg-laying chickens. Antimicrobial resistance and high antigenic diversity makes prevention and control by traditional treatments ineffective.

Actinobacillus pleuropneumoniae
Actinobacillus pleuropneumoniae (App) is a gram negative bacteria which infects pigs as its sole host and reservoir, causing severe economic losses to the livestock industry worldwide.

App is a widespread pathogen and it’s currently classified in 15 serotypes and 2 biovars, with a geographic serotype prevalence which greatly varies over time. The infection App provokes is a necrotizing bronchopneumoniae with a mortality rate ranging from 20% to 80 %, mainly depending on the serotype involved (Ser 1,5,9,10,11 most virulent). Currently available vaccines either confer a limited protection against just some of the App serotypes or are far too expensive to be adopted as a vast scale prophylaxis procedure.

Vaccine development
The approach is based on the concept of reverse vaccinology were bioinformatics tools and immunogenic screening is used to identify novel antigen. Two alternative vaccine strategies are currently used; one based on antigen delivery by adenoviral vectors or by antigen-enriched outer membrane vesicles naturally produced by both pathogens.

For more information, please contact Gry Persson (gryper@sund.ku.dk), Fabio Antenucci (fante@sund.ku.dk) or Anders Miki Bojesen (miki@sund.ku.dk).

Recent publications:

Kudirkiene, E, Christensen, H & Bojesen, AM 2013, 'Draft genome sequence ofGallibacterium anatis bv. haemolytica 12656-12 liver: an isolate obtained from the liver of a septicemic chicken' Genome Announcements, vol 1, no. 5, e00810-13.,10.1128/genomeA.00810-13

Bager, RJ, Kudirkiene, E, da Piedade, I, Seemann, T, Nielsen, TK, Pors, SE, Mattsson, AH, Boyce, JD, Adler, B & Bojesen, AM 2014, 'In silico prediction of Gallibacterium anatis pan-immunogens' Veterinary Research, vol 45, 80., 10.1186/s13567-014-0080-0

Kudirkiene, E, Bager, RJ, Johnson, TJ & Bojesen, AM 2014, 'Chaperone-usher fimbriae in a diverse selection of Gallibacterium genomes' B M C Genomics, vol 15, 1093.,10.1186/1471-2164-15-1093

Bager R.J., Persson G., Nesta B., Soriani M., Serino L., Jeppsson M., Nielsen T.K., Bojesen A.M. (2013): Outer membrane vesicles reflect environmental cues in Gallibacterium anatis. Vet Microbiol.167(3-4):565-72

Bager, R.J., Nesta, B., Pors, S.E., Soriani, M., Serino, L., Boyce, J.D., Adler, B., Bojesen, A.M. (2013): The fimbrial protein FlfA from Gallibacterium anatis is a virulence factor and vaccine candidate. Infect Immun. 81(6):1964-73