Identification of novel vaccine targets against G. anatis and A. pleuropneumoniae
The aim of our vaccine projects is to develop vaccine prototypes that offer protection in a serotype-independent manner.
G. anatis is an opportunistic pathogen that cause of salpingitis and peritonitis in egg-laying chickens. Antimicrobial resistance and high antigenic diversity makes prevention and control by traditional treatments ineffective.
Actinobacillus pleuropneumoniae (App) is a gram negative bacteria which infects pigs as its sole host and reservoir, causing severe economic losses to the livestock industry worldwide.
App is a widespread pathogen and it’s currently classified in 15 serotypes and 2 biovars, with a geographic serotype prevalence which greatly varies over time. The infection App provokes is a necrotizing bronchopneumoniae with a mortality rate ranging from 20% to 80 %, mainly depending on the serotype involved (Ser 1,5,9,10,11 most virulent). Currently available vaccines either confer a limited protection against just some of the App serotypes or are far too expensive to be adopted as a vast scale prophylaxis procedure.
The approach is based on the concept of reverse vaccinology were bioinformatics tools and immunogenic screening is used to identify novel antigen. Two alternative vaccine strategies are currently used; one based on antigen delivery by adenoviral vectors or by antigen-enriched outer membrane vesicles naturally produced by both pathogens.
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Bager, R.J., Nesta, B., Pors, S.E., Soriani, M., Serino, L., Boyce, J.D., Adler, B., Bojesen, A.M. (2013): The fimbrial protein FlfA from Gallibacterium anatis is a virulence factor and vaccine candidate. Infect Immun. 81(6):1964-73