Epigenetic and Transcriptomic Characterization of Pure Adipocyte Fractions From Obese Pigs Identifies Candidate Pathways Controlling Metabolism
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Epigenetic and Transcriptomic Characterization of Pure Adipocyte Fractions From Obese Pigs Identifies Candidate Pathways Controlling Metabolism. / Jacobsen, Mette Juul; Havgaard, Jakob H.; Anthon, Christian; Mentzel, Caroline M.Junker; Cirera, Susanna; Krogh, Poula Maltha; Pundhir, Sachin; Karlskov-Mortensen, Peter; Bruun, Camilla S.; Lesnik, Philippe; Guerin, Maryse; Gorodkin, Jan; Jørgensen, Claus B.; Fredholm, Merete; Barrès, Romain.
I: Frontiers in Genetics, Bind 10, 1268, 2019.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Epigenetic and Transcriptomic Characterization of Pure Adipocyte Fractions From Obese Pigs Identifies Candidate Pathways Controlling Metabolism
AU - Jacobsen, Mette Juul
AU - Havgaard, Jakob H.
AU - Anthon, Christian
AU - Mentzel, Caroline M.Junker
AU - Cirera, Susanna
AU - Krogh, Poula Maltha
AU - Pundhir, Sachin
AU - Karlskov-Mortensen, Peter
AU - Bruun, Camilla S.
AU - Lesnik, Philippe
AU - Guerin, Maryse
AU - Gorodkin, Jan
AU - Jørgensen, Claus B.
AU - Fredholm, Merete
AU - Barrès, Romain
PY - 2019
Y1 - 2019
N2 - Reprogramming of adipocyte function in obesity is implicated in metabolic disorders like type 2 diabetes. Here, we used the pig, an animal model sharing many physiological and pathophysiological similarities with humans, to perform in-depth epigenomic and transcriptomic characterization of pure adipocyte fractions. Using a combined DNA methylation capture sequencing and Reduced Representation bisulfite sequencing (RRBS) strategy in 11 lean and 12 obese pigs, we identified in 3529 differentially methylated regions (DMRs) located at close proximity to-, or within genes in the adipocytes. By sequencing of the transcriptome from the same fraction of isolated adipocytes, we identified 276 differentially expressed transcripts with at least one or more DMR. These transcripts were over-represented in gene pathways related to MAPK, metabolic and insulin signaling. Using a candidate gene approach, we further characterized 13 genes potentially regulated by DNA methylation and identified putative transcription factor binding sites that could be affected by the differential methylation in obesity. Our data constitute a valuable resource for further investigations aiming to delineate the epigenetic etiology of metabolic disorders.
AB - Reprogramming of adipocyte function in obesity is implicated in metabolic disorders like type 2 diabetes. Here, we used the pig, an animal model sharing many physiological and pathophysiological similarities with humans, to perform in-depth epigenomic and transcriptomic characterization of pure adipocyte fractions. Using a combined DNA methylation capture sequencing and Reduced Representation bisulfite sequencing (RRBS) strategy in 11 lean and 12 obese pigs, we identified in 3529 differentially methylated regions (DMRs) located at close proximity to-, or within genes in the adipocytes. By sequencing of the transcriptome from the same fraction of isolated adipocytes, we identified 276 differentially expressed transcripts with at least one or more DMR. These transcripts were over-represented in gene pathways related to MAPK, metabolic and insulin signaling. Using a candidate gene approach, we further characterized 13 genes potentially regulated by DNA methylation and identified putative transcription factor binding sites that could be affected by the differential methylation in obesity. Our data constitute a valuable resource for further investigations aiming to delineate the epigenetic etiology of metabolic disorders.
KW - DNA methylation
KW - epigenetics
KW - metabolism
KW - obesity
KW - RNAseq
KW - Sus scrofa
U2 - 10.3389/fgene.2019.01268
DO - 10.3389/fgene.2019.01268
M3 - Journal article
C2 - 31921306
AN - SCOPUS:85077317504
VL - 10
JO - Frontiers in Genetics
JF - Frontiers in Genetics
SN - 1664-8021
M1 - 1268
ER -
ID: 234207706