TY - JOUR

T1 - Salmonella Heidelberg and Salmonella Minnesota in Brazilian broilers

T2 - Genomic characterization of third-generation cephalosporin and fluoroquinolone-resistant strains

AU - Saidenberg, Andre Becker Simoes

AU - Franco, Leticia Soares

AU - Reple, Jessica Nacarato

AU - Hounmanou, Yaovi Mahuton Gildas

AU - Casas, Monique Ribeiro Tiba

AU - Cardoso, Brenda

AU - Esposito, Fernanda

AU - Lincopan, Nilton

AU - Dalsgaard, Anders

AU - Stegger, Marc

AU - Knöbl, Terezinha

N1 - © 2022 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd.

PY - 2023

Y1 - 2023

N2 - Salmonella serovars Heidelberg and Minnesota encoding antimicrobial resistance to third-generation cephalosporins and fluoroquinolones are often detected in poultry/poultry meat. We analysed the genomes of 10 Salmonella Heidelberg (SH) and 4 Salmonella Minnesota (SM) from faecal isolates of Brazilian poultry. These featured virulent and multidrug-resistant characteristics, with AmpC beta-lactamase (blaCMY-2 ) predominance (9/14), for all SM (4/4) and some SH (3/10) located on IncC plasmid replicons. IncC carrying blaCTX-M-2 was only detected among SH (3/10). Mutation in the gyrA/parC genes was present in all SH, whereas SM harboured parC mutation plus qnrB19 on ColRNAI plasmids (3/4). In silico resistance overall corroborated with phenotypic results. Core genome phylogenies showed close clustering and high similarities between the Brazilian and poultry meat/food isolates from Europe, and to human isolates from European countries with documented import of Brazilian poultry meat. Conjugation assays with SM successfully transferred blaCMY-2 , and qnrB19 to an Escherichia coli recipient. The findings reinforce the ongoing antimicrobial resistance acquisition of SH and Minnesota and the risks for disseminating resistant strains and/or mobile elements which may increasingly affect importing countries and the need for controlling AMR in major poultry-exporting countries like Brazil.

AB - Salmonella serovars Heidelberg and Minnesota encoding antimicrobial resistance to third-generation cephalosporins and fluoroquinolones are often detected in poultry/poultry meat. We analysed the genomes of 10 Salmonella Heidelberg (SH) and 4 Salmonella Minnesota (SM) from faecal isolates of Brazilian poultry. These featured virulent and multidrug-resistant characteristics, with AmpC beta-lactamase (blaCMY-2 ) predominance (9/14), for all SM (4/4) and some SH (3/10) located on IncC plasmid replicons. IncC carrying blaCTX-M-2 was only detected among SH (3/10). Mutation in the gyrA/parC genes was present in all SH, whereas SM harboured parC mutation plus qnrB19 on ColRNAI plasmids (3/4). In silico resistance overall corroborated with phenotypic results. Core genome phylogenies showed close clustering and high similarities between the Brazilian and poultry meat/food isolates from Europe, and to human isolates from European countries with documented import of Brazilian poultry meat. Conjugation assays with SM successfully transferred blaCMY-2 , and qnrB19 to an Escherichia coli recipient. The findings reinforce the ongoing antimicrobial resistance acquisition of SH and Minnesota and the risks for disseminating resistant strains and/or mobile elements which may increasingly affect importing countries and the need for controlling AMR in major poultry-exporting countries like Brazil.

U2 - 10.1111/1758-2229.13132

DO - 10.1111/1758-2229.13132

M3 - Journal article

C2 - 36629129

VL - 15

SP - 119

EP - 128

JO - Environmental Microbiology Reports

JF - Environmental Microbiology Reports

SN - 1758-2229

IS - 2

ER -