Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance
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Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance. / Sørensen, Martine C.Holst; Vitt, Amira; Neve, Horst; Soverini, Matteo; Ahern, Stephen James; Klumpp, Jochen; Brøndsted, Lone.
In: Cell Reports, Vol. 35, No. 10, 109214, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance
AU - Sørensen, Martine C.Holst
AU - Vitt, Amira
AU - Neve, Horst
AU - Soverini, Matteo
AU - Ahern, Stephen James
AU - Klumpp, Jochen
AU - Brøndsted, Lone
N1 - Publisher Copyright: © 2021 The Authors
PY - 2021
Y1 - 2021
N2 - Phase variation is a common mechanism for creating phenotypic heterogeneity of surface structures in bacteria important for niche adaptation. In Campylobacter, phase variation occurs by random variation in hypermutable homonucleotide 7–11 G (polyG) tracts. To elucidate how phages adapt to phase-variable hosts, we study Fletchervirus phages infecting Campylobacter dependent on a phase-variable receptor. Our data demonstrate that Fletcherviruses mimic their host and encode hypermutable polyG tracts, leading to phase-variable expression of two of four receptor-binding proteins. This creates phenotypically diverse phage populations, including a sub-population that infects the bacterial host when the phase-variable receptor is not expressed. Such population dynamics of both phage and host promote co-existence in a shared niche. Strikingly, we identify polyG tracts in more than 100 phage genera, infecting more than 70 bacterial species. Future experimental work may confirm phase variation as a widespread strategy for creating phenotypically diverse phage populations.
AB - Phase variation is a common mechanism for creating phenotypic heterogeneity of surface structures in bacteria important for niche adaptation. In Campylobacter, phase variation occurs by random variation in hypermutable homonucleotide 7–11 G (polyG) tracts. To elucidate how phages adapt to phase-variable hosts, we study Fletchervirus phages infecting Campylobacter dependent on a phase-variable receptor. Our data demonstrate that Fletcherviruses mimic their host and encode hypermutable polyG tracts, leading to phase-variable expression of two of four receptor-binding proteins. This creates phenotypically diverse phage populations, including a sub-population that infects the bacterial host when the phase-variable receptor is not expressed. Such population dynamics of both phage and host promote co-existence in a shared niche. Strikingly, we identify polyG tracts in more than 100 phage genera, infecting more than 70 bacterial species. Future experimental work may confirm phase variation as a widespread strategy for creating phenotypically diverse phage populations.
KW - Campylobacter
KW - Fletchervirus
KW - MeOPN
KW - phage receptor
KW - phage resistance
KW - phages
KW - phase variation
KW - polyG tracts
KW - receptor binding proteins
U2 - 10.1016/j.celrep.2021.109214
DO - 10.1016/j.celrep.2021.109214
M3 - Journal article
C2 - 34107245
AN - SCOPUS:85107426535
VL - 35
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 10
M1 - 109214
ER -
ID: 272113768