Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs
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Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs. / Græsbøll, Kaare; Larsen, Inge; Clasen, Julie; Birkegård, Anna Camilla; Nielsen, Jens Peter; Christiansen, Lasse Engbo; Olsen, John Elmerdahl; Angen, Øystein; Folkesson, Anders.
I: BMC Microbiology, Bind 19, Nr. 1, 269, 2019.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Effect of tetracycline treatment regimens on antibiotic resistance gene selection over time in nursery pigs
AU - Græsbøll, Kaare
AU - Larsen, Inge
AU - Clasen, Julie
AU - Birkegård, Anna Camilla
AU - Nielsen, Jens Peter
AU - Christiansen, Lasse Engbo
AU - Olsen, John Elmerdahl
AU - Angen, Øystein
AU - Folkesson, Anders
PY - 2019
Y1 - 2019
N2 - Background: The majority of antimicrobials given during the production of pigs are given to nursery pigs. The influence of antimicrobial use on the levels of antimicrobial resistant (AMR) genes is important to quantify to be able to assess the impact of resistance on the food chain and risk to human and animal health. Results: This study investigated the response on the levels of nine AMR genes to five different treatment strategies with oxytetracycline, and the dynamics of gene abundance over time by following 1167 pigs from five different farms in Denmark. The results showed no significant difference between treatments and an increase in abundance for the efflux pump encoding tet(A) gene and the genes encoding the ribosomal protection proteins tet(O) and tet(W) tetracycline resistant genes following treatment, while tet(M) showed no response to treatment. However, it was also observed that the levels of tet(O), tet(W), and ermB in some farms would drift more over time compared to a single treatment-course with antibiotic. Conclusion: This study underlines the large variation in AMR levels under natural conditions and the need for increased investigation of the complex interactions of antimicrobial treatment and other environmental and managerial practices in swine production on AMR gene abundance.
AB - Background: The majority of antimicrobials given during the production of pigs are given to nursery pigs. The influence of antimicrobial use on the levels of antimicrobial resistant (AMR) genes is important to quantify to be able to assess the impact of resistance on the food chain and risk to human and animal health. Results: This study investigated the response on the levels of nine AMR genes to five different treatment strategies with oxytetracycline, and the dynamics of gene abundance over time by following 1167 pigs from five different farms in Denmark. The results showed no significant difference between treatments and an increase in abundance for the efflux pump encoding tet(A) gene and the genes encoding the ribosomal protection proteins tet(O) and tet(W) tetracycline resistant genes following treatment, while tet(M) showed no response to treatment. However, it was also observed that the levels of tet(O), tet(W), and ermB in some farms would drift more over time compared to a single treatment-course with antibiotic. Conclusion: This study underlines the large variation in AMR levels under natural conditions and the need for increased investigation of the complex interactions of antimicrobial treatment and other environmental and managerial practices in swine production on AMR gene abundance.
KW - Nursery pigs
KW - Oxytetracycline
KW - qPCR
KW - Resistance genes
U2 - 10.1186/s12866-019-1619-z
DO - 10.1186/s12866-019-1619-z
M3 - Journal article
C2 - 31791243
AN - SCOPUS:85075926251
VL - 19
JO - BMC Microbiology
JF - BMC Microbiology
SN - 1471-2180
IS - 1
M1 - 269
ER -
ID: 234213976