Fibrinolytic and antibiotic treatment of prosthetic vascular graft infections in a novel rat model
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Fibrinolytic and antibiotic treatment of prosthetic vascular graft infections in a novel rat model. / Johansen, Mikkel Illemann; Rahbek, Søren Jensen; Jensen-Fangel, Søren; Minero, Gabriel Antonio S.; Jensen, Louise Kruse; Larsen, Ole Halfdan; Erikstrup, Lise Tornvig; Seefeldt, Anders Marthinsen; Østergaard, Lars; Meyer, Rikke Louise; Jørgensen, Nis Pedersen.
I: PLoS ONE, Bind 18, e0287671, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Fibrinolytic and antibiotic treatment of prosthetic vascular graft infections in a novel rat model
AU - Johansen, Mikkel Illemann
AU - Rahbek, Søren Jensen
AU - Jensen-Fangel, Søren
AU - Minero, Gabriel Antonio S.
AU - Jensen, Louise Kruse
AU - Larsen, Ole Halfdan
AU - Erikstrup, Lise Tornvig
AU - Seefeldt, Anders Marthinsen
AU - Østergaard, Lars
AU - Meyer, Rikke Louise
AU - Jørgensen, Nis Pedersen
N1 - Publisher Copyright: © 2023 Johansen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2023
Y1 - 2023
N2 - Objectives We developed a rat model of prosthetic vascular graft infection to assess, whether the fibrinolytic tissue plasminogen activator (tPA) could increase the efficacy of antibiotic therapy. Materials and methods Rats were implanted a polyethylene graft in the common carotid artery, pre-inoculated with approx. 6 log10 colony forming units (CFU) of methicillin resistant Staphylococcus aureus. Ten days after surgery, rats were randomized to either: 0.9% NaCl (n = 8), vancomycin (n = 8), vancomycin + tPA (n = 8), vancomycin + rifampicin (n = 18) or vancomycin + rifampicin + tPA (n = 18). Treatment duration was seven days. Approximately 36 hours after the end of treatment, the rats were euthanized, and grafts and organs were harvested for CFU enumeration. Results All animals in the control group had significantly higher CFU at the time of euthanization compared to bacterial load found on the grafts prior to inoculation (6.45 vs. 4.36 mean log10 CFU/mL, p = 0.0011), and both the procedure and infection were well tolerated. Vancomycin and rifampicin treatment were superior to monotherapy with vancomycin, as it lead to a marked decrease in median bacterial load on the grafts (3.50 vs. 6.56 log10 CFU/ mL, p = 0.0016). The addition of tPA to vancomycin and rifampicin combination treatment did not show a further decrease in bacterial load (4.078 vs. 3.50 log10 CFU/mL, p = 0.26). The cure rate was 16% in the vancomycin + rifampicin group vs. 37.5% cure rate in the.
AB - Objectives We developed a rat model of prosthetic vascular graft infection to assess, whether the fibrinolytic tissue plasminogen activator (tPA) could increase the efficacy of antibiotic therapy. Materials and methods Rats were implanted a polyethylene graft in the common carotid artery, pre-inoculated with approx. 6 log10 colony forming units (CFU) of methicillin resistant Staphylococcus aureus. Ten days after surgery, rats were randomized to either: 0.9% NaCl (n = 8), vancomycin (n = 8), vancomycin + tPA (n = 8), vancomycin + rifampicin (n = 18) or vancomycin + rifampicin + tPA (n = 18). Treatment duration was seven days. Approximately 36 hours after the end of treatment, the rats were euthanized, and grafts and organs were harvested for CFU enumeration. Results All animals in the control group had significantly higher CFU at the time of euthanization compared to bacterial load found on the grafts prior to inoculation (6.45 vs. 4.36 mean log10 CFU/mL, p = 0.0011), and both the procedure and infection were well tolerated. Vancomycin and rifampicin treatment were superior to monotherapy with vancomycin, as it lead to a marked decrease in median bacterial load on the grafts (3.50 vs. 6.56 log10 CFU/ mL, p = 0.0016). The addition of tPA to vancomycin and rifampicin combination treatment did not show a further decrease in bacterial load (4.078 vs. 3.50 log10 CFU/mL, p = 0.26). The cure rate was 16% in the vancomycin + rifampicin group vs. 37.5% cure rate in the.
U2 - 10.1371/journal.pone.0287671
DO - 10.1371/journal.pone.0287671
M3 - Journal article
C2 - 37463137
AN - SCOPUS:85165518219
VL - 18
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
M1 - e0287671
ER -
ID: 365822195