β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus. / Zhang, Meng; Lai, Yiwei; Krupalnik, Vladislav; Guo, Pengcheng; Guo, Xiangpeng; Zhou, Jianguo; Xu, Yan; Yu, Zhijun; Liu, Longqi; Jiang, Ao; Li, Wenjuan; Abdul, Mazid Md; Ma, Gang; Li, Na; Fu, Xiuling; Lv, Yuan; Jiang, Mengling; Tariq, Muqddas; Kanwal, Shahzina; Liu, Hao; Xu, Xueting; Zhang, Hui; Huang, Yinghua; Wang, Lulu; Chen, Shuhan; Babarinde, Isaac A.; Luo, Zhiwei; Wang, Dongye; Zhou, Tiantian; Ward, Carl; He, Minghui; Ibañez, David P.; Li, Yunpan; Zhou, Jiajian; Yuan, Jie; Feng, Yayan; Arumugam, Karthik; Di Vicino, Umberto; Bao, Xichen; Wu, Guangming; Schambach, Axel; Wang, Huating; Sun, Hao; Gao, Fei; Qin, Baoming; Hutchins, Andrew P.; Doble, Bradley W.; Hartmann, Christine; Cosma, Maria Pia; Qin, Yan; Xu, Guo Liang; Chen, Runsheng; Volpe, Giacomo; Chen, Liang; Hanna, Jacob H.; Esteban, Miguel A.

In: Science Advances, Vol. 6, No. 29, eaba1593, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, M, Lai, Y, Krupalnik, V, Guo, P, Guo, X, Zhou, J, Xu, Y, Yu, Z, Liu, L, Jiang, A, Li, W, Abdul, MM, Ma, G, Li, N, Fu, X, Lv, Y, Jiang, M, Tariq, M, Kanwal, S, Liu, H, Xu, X, Zhang, H, Huang, Y, Wang, L, Chen, S, Babarinde, IA, Luo, Z, Wang, D, Zhou, T, Ward, C, He, M, Ibañez, DP, Li, Y, Zhou, J, Yuan, J, Feng, Y, Arumugam, K, Di Vicino, U, Bao, X, Wu, G, Schambach, A, Wang, H, Sun, H, Gao, F, Qin, B, Hutchins, AP, Doble, BW, Hartmann, C, Cosma, MP, Qin, Y, Xu, GL, Chen, R, Volpe, G, Chen, L, Hanna, JH & Esteban, MA 2020, 'β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus', Science Advances, vol. 6, no. 29, eaba1593. https://doi.org/10.1126/sciadv.aba1593

APA

Zhang, M., Lai, Y., Krupalnik, V., Guo, P., Guo, X., Zhou, J., Xu, Y., Yu, Z., Liu, L., Jiang, A., Li, W., Abdul, M. M., Ma, G., Li, N., Fu, X., Lv, Y., Jiang, M., Tariq, M., Kanwal, S., ... Esteban, M. A. (2020). β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus. Science Advances, 6(29), [eaba1593]. https://doi.org/10.1126/sciadv.aba1593

Vancouver

Zhang M, Lai Y, Krupalnik V, Guo P, Guo X, Zhou J et al. β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus. Science Advances. 2020;6(29). eaba1593. https://doi.org/10.1126/sciadv.aba1593

Author

Zhang, Meng ; Lai, Yiwei ; Krupalnik, Vladislav ; Guo, Pengcheng ; Guo, Xiangpeng ; Zhou, Jianguo ; Xu, Yan ; Yu, Zhijun ; Liu, Longqi ; Jiang, Ao ; Li, Wenjuan ; Abdul, Mazid Md ; Ma, Gang ; Li, Na ; Fu, Xiuling ; Lv, Yuan ; Jiang, Mengling ; Tariq, Muqddas ; Kanwal, Shahzina ; Liu, Hao ; Xu, Xueting ; Zhang, Hui ; Huang, Yinghua ; Wang, Lulu ; Chen, Shuhan ; Babarinde, Isaac A. ; Luo, Zhiwei ; Wang, Dongye ; Zhou, Tiantian ; Ward, Carl ; He, Minghui ; Ibañez, David P. ; Li, Yunpan ; Zhou, Jiajian ; Yuan, Jie ; Feng, Yayan ; Arumugam, Karthik ; Di Vicino, Umberto ; Bao, Xichen ; Wu, Guangming ; Schambach, Axel ; Wang, Huating ; Sun, Hao ; Gao, Fei ; Qin, Baoming ; Hutchins, Andrew P. ; Doble, Bradley W. ; Hartmann, Christine ; Cosma, Maria Pia ; Qin, Yan ; Xu, Guo Liang ; Chen, Runsheng ; Volpe, Giacomo ; Chen, Liang ; Hanna, Jacob H. ; Esteban, Miguel A. / β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus. In: Science Advances. 2020 ; Vol. 6, No. 29.

Bibtex

@article{ea0c514e4c4e4222bfe1e57f1504a1b4,
title = "β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus",
abstract = "Mouse embryonic stem cells cultured with MEK (mitogen-activated protein kinase kinase) and GSK3 (glycogen synthase kinase 3) inhibitors (2i) more closely resemble the inner cell mass of preimplantation blastocysts than those cultured with SL [serum/leukemia inhibitory factor (LIF)]. The transcriptional mechanisms governing this pluripotent ground state are unresolved. Release of promoter-proximal paused RNA polymerase II (Pol2) is a multistep process necessary for pluripotency and cell cycle gene transcription in SL. We show that β-catenin, stabilized by GSK3 inhibition in medium with 2i, supplies transcriptional coregulators at pluripotency loci. This selectively strengthens pluripotency loci and renders them addicted to transcription initiation for productive gene body elongation in detriment to Pol2 pause release. By contrast, cell cycle genes are not bound by β-catenin, and proliferation/self-renewal remains tightly controlled by Pol2 pause release under 2i conditions. Our findings explain how pluripotency is reinforced in the ground state and also provide a general model for transcriptional resilience/ adaptation upon network perturbation in other contexts.",
author = "Meng Zhang and Yiwei Lai and Vladislav Krupalnik and Pengcheng Guo and Xiangpeng Guo and Jianguo Zhou and Yan Xu and Zhijun Yu and Longqi Liu and Ao Jiang and Wenjuan Li and Abdul, {Mazid Md} and Gang Ma and Na Li and Xiuling Fu and Yuan Lv and Mengling Jiang and Muqddas Tariq and Shahzina Kanwal and Hao Liu and Xueting Xu and Hui Zhang and Yinghua Huang and Lulu Wang and Shuhan Chen and Babarinde, {Isaac A.} and Zhiwei Luo and Dongye Wang and Tiantian Zhou and Carl Ward and Minghui He and Iba{\~n}ez, {David P.} and Yunpan Li and Jiajian Zhou and Jie Yuan and Yayan Feng and Karthik Arumugam and {Di Vicino}, Umberto and Xichen Bao and Guangming Wu and Axel Schambach and Huating Wang and Hao Sun and Fei Gao and Baoming Qin and Hutchins, {Andrew P.} and Doble, {Bradley W.} and Christine Hartmann and Cosma, {Maria Pia} and Yan Qin and Xu, {Guo Liang} and Runsheng Chen and Giacomo Volpe and Liang Chen and Hanna, {Jacob H.} and Esteban, {Miguel A.}",
year = "2020",
doi = "10.1126/sciadv.aba1593",
language = "English",
volume = "6",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "29",

}

RIS

TY - JOUR

T1 - β-Catenin safeguards the ground state of mouse pluripotency by strengthening the robustness of the transcriptional apparatus

AU - Zhang, Meng

AU - Lai, Yiwei

AU - Krupalnik, Vladislav

AU - Guo, Pengcheng

AU - Guo, Xiangpeng

AU - Zhou, Jianguo

AU - Xu, Yan

AU - Yu, Zhijun

AU - Liu, Longqi

AU - Jiang, Ao

AU - Li, Wenjuan

AU - Abdul, Mazid Md

AU - Ma, Gang

AU - Li, Na

AU - Fu, Xiuling

AU - Lv, Yuan

AU - Jiang, Mengling

AU - Tariq, Muqddas

AU - Kanwal, Shahzina

AU - Liu, Hao

AU - Xu, Xueting

AU - Zhang, Hui

AU - Huang, Yinghua

AU - Wang, Lulu

AU - Chen, Shuhan

AU - Babarinde, Isaac A.

AU - Luo, Zhiwei

AU - Wang, Dongye

AU - Zhou, Tiantian

AU - Ward, Carl

AU - He, Minghui

AU - Ibañez, David P.

AU - Li, Yunpan

AU - Zhou, Jiajian

AU - Yuan, Jie

AU - Feng, Yayan

AU - Arumugam, Karthik

AU - Di Vicino, Umberto

AU - Bao, Xichen

AU - Wu, Guangming

AU - Schambach, Axel

AU - Wang, Huating

AU - Sun, Hao

AU - Gao, Fei

AU - Qin, Baoming

AU - Hutchins, Andrew P.

AU - Doble, Bradley W.

AU - Hartmann, Christine

AU - Cosma, Maria Pia

AU - Qin, Yan

AU - Xu, Guo Liang

AU - Chen, Runsheng

AU - Volpe, Giacomo

AU - Chen, Liang

AU - Hanna, Jacob H.

AU - Esteban, Miguel A.

PY - 2020

Y1 - 2020

N2 - Mouse embryonic stem cells cultured with MEK (mitogen-activated protein kinase kinase) and GSK3 (glycogen synthase kinase 3) inhibitors (2i) more closely resemble the inner cell mass of preimplantation blastocysts than those cultured with SL [serum/leukemia inhibitory factor (LIF)]. The transcriptional mechanisms governing this pluripotent ground state are unresolved. Release of promoter-proximal paused RNA polymerase II (Pol2) is a multistep process necessary for pluripotency and cell cycle gene transcription in SL. We show that β-catenin, stabilized by GSK3 inhibition in medium with 2i, supplies transcriptional coregulators at pluripotency loci. This selectively strengthens pluripotency loci and renders them addicted to transcription initiation for productive gene body elongation in detriment to Pol2 pause release. By contrast, cell cycle genes are not bound by β-catenin, and proliferation/self-renewal remains tightly controlled by Pol2 pause release under 2i conditions. Our findings explain how pluripotency is reinforced in the ground state and also provide a general model for transcriptional resilience/ adaptation upon network perturbation in other contexts.

AB - Mouse embryonic stem cells cultured with MEK (mitogen-activated protein kinase kinase) and GSK3 (glycogen synthase kinase 3) inhibitors (2i) more closely resemble the inner cell mass of preimplantation blastocysts than those cultured with SL [serum/leukemia inhibitory factor (LIF)]. The transcriptional mechanisms governing this pluripotent ground state are unresolved. Release of promoter-proximal paused RNA polymerase II (Pol2) is a multistep process necessary for pluripotency and cell cycle gene transcription in SL. We show that β-catenin, stabilized by GSK3 inhibition in medium with 2i, supplies transcriptional coregulators at pluripotency loci. This selectively strengthens pluripotency loci and renders them addicted to transcription initiation for productive gene body elongation in detriment to Pol2 pause release. By contrast, cell cycle genes are not bound by β-catenin, and proliferation/self-renewal remains tightly controlled by Pol2 pause release under 2i conditions. Our findings explain how pluripotency is reinforced in the ground state and also provide a general model for transcriptional resilience/ adaptation upon network perturbation in other contexts.

U2 - 10.1126/sciadv.aba1593

DO - 10.1126/sciadv.aba1593

M3 - Journal article

C2 - 32832621

AN - SCOPUS:85090069337

VL - 6

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 29

M1 - eaba1593

ER -

ID: 248461817