Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils

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Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils. / Jacobsen, Mariann Juul; Nielsen, Jens Peter.

In: Veterinary Microbiology, Vol. 47, No. 1-2, 11.1995, p. 191-197.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jacobsen, MJ & Nielsen, JP 1995, 'Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils', Veterinary Microbiology, vol. 47, no. 1-2, pp. 191-197. https://doi.org/10.1016/0378-1135(95)00062-F

APA

Jacobsen, M. J., & Nielsen, J. P. (1995). Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils. Veterinary Microbiology, 47(1-2), 191-197. https://doi.org/10.1016/0378-1135(95)00062-F

Vancouver

Jacobsen MJ, Nielsen JP. Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils. Veterinary Microbiology. 1995 Nov;47(1-2):191-197. https://doi.org/10.1016/0378-1135(95)00062-F

Author

Jacobsen, Mariann Juul ; Nielsen, Jens Peter. / Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils. In: Veterinary Microbiology. 1995 ; Vol. 47, No. 1-2. pp. 191-197.

Bibtex

@article{a6ff0ebd210f4ca2bb9d4d07d52bbc2f,
title = "Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils",
abstract = "In order to isolate Actinobacillus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumoniae were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.",
keywords = "Actinobacillus pleuropneumoniae, culture methods, Indicative agar, Pig, Selective agar, tonsil",
author = "Jacobsen, {Mariann Juul} and Nielsen, {Jens Peter}",
year = "1995",
month = nov,
doi = "10.1016/0378-1135(95)00062-F",
language = "English",
volume = "47",
pages = "191--197",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils

AU - Jacobsen, Mariann Juul

AU - Nielsen, Jens Peter

PY - 1995/11

Y1 - 1995/11

N2 - In order to isolate Actinobacillus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumoniae were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.

AB - In order to isolate Actinobacillus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumoniae were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.

KW - Actinobacillus pleuropneumoniae

KW - culture methods

KW - Indicative agar

KW - Pig

KW - Selective agar

KW - tonsil

UR - http://www.scopus.com/inward/record.url?scp=0028868813&partnerID=8YFLogxK

U2 - 10.1016/0378-1135(95)00062-F

DO - 10.1016/0378-1135(95)00062-F

M3 - Journal article

C2 - 8604551

AN - SCOPUS:0028868813

VL - 47

SP - 191

EP - 197

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

ER -

ID: 285716382