DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark

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DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark. / Damborg, Peter; Moodley, Arshnee; Aalbaek, Bent; Ventrella, Gianpiero; dos Santos, Teresa Pires; Guardabassi, Luca.

In: BMC Veterinary Research, Vol. 12, 131, 29.06.2016.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Damborg, P, Moodley, A, Aalbaek, B, Ventrella, G, dos Santos, TP & Guardabassi, L 2016, 'DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark', BMC Veterinary Research, vol. 12, 131. https://doi.org/10.1186/s12917-016-0756-y

APA

Damborg, P., Moodley, A., Aalbaek, B., Ventrella, G., dos Santos, T. P., & Guardabassi, L. (2016). DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark. BMC Veterinary Research, 12, [131]. https://doi.org/10.1186/s12917-016-0756-y

Vancouver

Damborg P, Moodley A, Aalbaek B, Ventrella G, dos Santos TP, Guardabassi L. DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark. BMC Veterinary Research. 2016 Jun 29;12. 131. https://doi.org/10.1186/s12917-016-0756-y

Author

Damborg, Peter ; Moodley, Arshnee ; Aalbaek, Bent ; Ventrella, Gianpiero ; dos Santos, Teresa Pires ; Guardabassi, Luca. / DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark. In: BMC Veterinary Research. 2016 ; Vol. 12.

Bibtex

@article{3d4c76a021874875bea974c89166497a,
title = "DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark",
abstract = "Background: Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged globally in companion animals in the last decade. In Europe, the multidrug-resistant sequence type (ST) 71 is widespread, but recently other clones have appeared. The objective of this study was to examine genotypic diversity and antimicrobial resistance of clinical MRSP isolates obtained from dogs, including dogs sampled on multiple occasions, in Denmark over a six-year period. For that purpose a total of 46 clinical MRSP isolates obtained from 36 dogs between 2009 and 2014 were subjected to antimicrobial susceptibility testing, multilocus-sequence typing (MLST) and SCCmec typing.Results: Twenty-three sequence types were identified with ST71, mostly associated with SCCmec II-III, as the most common occurring in 13 dogs. Among the remaining 33 isolates, 19 belonged to clonal complex (CC) 258 comprising ST258-SCCmec IV and its single-and double-locus variants. These were susceptible to 4-7 of the 22 antibiotics tested, whereas CC71 isolates were susceptible to only 2-5 antibiotics. Clone-specific differences were especially pronounced for fluoroquinolones and aminoglycosides with most CC71 isolates being resistant and almost all CC258 isolates being susceptible. Sixteen of the 19 CC258 isolates had oxacillin MICs of 0.5 g/L, whereas MICs for CC71 isolates were consistently above 4 g/L. Four of five dogs representing multiple isolates had distinct STs on different sampling events.Conclusions: The overall genotypic diversity of MRSP is high in Denmark indicating multiple acquisitions of SCCmec into distinct clones, and mutational evolution, which appears to be particularly rapid for certain ancestral clones such as ST258. ST71-SCCmec II-III is the most common MRSP lineage and is typically multidrug-resistant. CC258-SCCmec IV isolates, which emerged in Denmark since 2012, display susceptibility to a wider range of antimicrobials. The isolation of distinct STs in individual dogs over time suggests repeated exposure or short-term genetic evolution of MRSP clones within patients.",
keywords = "MRSP, Dog, Antibiotic resistance, Multilocus sequence typing, ST71, ST258",
author = "Peter Damborg and Arshnee Moodley and Bent Aalbaek and Gianpiero Ventrella and {dos Santos}, {Teresa Pires} and Luca Guardabassi",
year = "2016",
month = jun,
day = "29",
doi = "10.1186/s12917-016-0756-y",
language = "English",
volume = "12",
journal = "B M C Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - DQ High genotypic diversity among methicillin-resistant Staphylococcus pseudintermedius isolated from canine infections in Denmark

AU - Damborg, Peter

AU - Moodley, Arshnee

AU - Aalbaek, Bent

AU - Ventrella, Gianpiero

AU - dos Santos, Teresa Pires

AU - Guardabassi, Luca

PY - 2016/6/29

Y1 - 2016/6/29

N2 - Background: Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged globally in companion animals in the last decade. In Europe, the multidrug-resistant sequence type (ST) 71 is widespread, but recently other clones have appeared. The objective of this study was to examine genotypic diversity and antimicrobial resistance of clinical MRSP isolates obtained from dogs, including dogs sampled on multiple occasions, in Denmark over a six-year period. For that purpose a total of 46 clinical MRSP isolates obtained from 36 dogs between 2009 and 2014 were subjected to antimicrobial susceptibility testing, multilocus-sequence typing (MLST) and SCCmec typing.Results: Twenty-three sequence types were identified with ST71, mostly associated with SCCmec II-III, as the most common occurring in 13 dogs. Among the remaining 33 isolates, 19 belonged to clonal complex (CC) 258 comprising ST258-SCCmec IV and its single-and double-locus variants. These were susceptible to 4-7 of the 22 antibiotics tested, whereas CC71 isolates were susceptible to only 2-5 antibiotics. Clone-specific differences were especially pronounced for fluoroquinolones and aminoglycosides with most CC71 isolates being resistant and almost all CC258 isolates being susceptible. Sixteen of the 19 CC258 isolates had oxacillin MICs of 0.5 g/L, whereas MICs for CC71 isolates were consistently above 4 g/L. Four of five dogs representing multiple isolates had distinct STs on different sampling events.Conclusions: The overall genotypic diversity of MRSP is high in Denmark indicating multiple acquisitions of SCCmec into distinct clones, and mutational evolution, which appears to be particularly rapid for certain ancestral clones such as ST258. ST71-SCCmec II-III is the most common MRSP lineage and is typically multidrug-resistant. CC258-SCCmec IV isolates, which emerged in Denmark since 2012, display susceptibility to a wider range of antimicrobials. The isolation of distinct STs in individual dogs over time suggests repeated exposure or short-term genetic evolution of MRSP clones within patients.

AB - Background: Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged globally in companion animals in the last decade. In Europe, the multidrug-resistant sequence type (ST) 71 is widespread, but recently other clones have appeared. The objective of this study was to examine genotypic diversity and antimicrobial resistance of clinical MRSP isolates obtained from dogs, including dogs sampled on multiple occasions, in Denmark over a six-year period. For that purpose a total of 46 clinical MRSP isolates obtained from 36 dogs between 2009 and 2014 were subjected to antimicrobial susceptibility testing, multilocus-sequence typing (MLST) and SCCmec typing.Results: Twenty-three sequence types were identified with ST71, mostly associated with SCCmec II-III, as the most common occurring in 13 dogs. Among the remaining 33 isolates, 19 belonged to clonal complex (CC) 258 comprising ST258-SCCmec IV and its single-and double-locus variants. These were susceptible to 4-7 of the 22 antibiotics tested, whereas CC71 isolates were susceptible to only 2-5 antibiotics. Clone-specific differences were especially pronounced for fluoroquinolones and aminoglycosides with most CC71 isolates being resistant and almost all CC258 isolates being susceptible. Sixteen of the 19 CC258 isolates had oxacillin MICs of 0.5 g/L, whereas MICs for CC71 isolates were consistently above 4 g/L. Four of five dogs representing multiple isolates had distinct STs on different sampling events.Conclusions: The overall genotypic diversity of MRSP is high in Denmark indicating multiple acquisitions of SCCmec into distinct clones, and mutational evolution, which appears to be particularly rapid for certain ancestral clones such as ST258. ST71-SCCmec II-III is the most common MRSP lineage and is typically multidrug-resistant. CC258-SCCmec IV isolates, which emerged in Denmark since 2012, display susceptibility to a wider range of antimicrobials. The isolation of distinct STs in individual dogs over time suggests repeated exposure or short-term genetic evolution of MRSP clones within patients.

KW - MRSP

KW - Dog

KW - Antibiotic resistance

KW - Multilocus sequence typing

KW - ST71

KW - ST258

U2 - 10.1186/s12917-016-0756-y

DO - 10.1186/s12917-016-0756-y

M3 - Journal article

C2 - 27357502

VL - 12

JO - B M C Veterinary Research

JF - B M C Veterinary Research

SN - 1746-6148

M1 - 131

ER -

ID: 165743640