First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania

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First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania. / Katakweba, Abdul A.S.; Muhairwa, Amandus P.; Lupindu, Athumani M.; Damborg, Peter; Rosenkrantz, Jesper T.; Minga, Uswege M.; Mtambo, Madundo M.A.; Olsen, John E.

In: Microbial Drug Resistance, Vol. 24, No. 3, 2018, p. 260-268.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Katakweba, AAS, Muhairwa, AP, Lupindu, AM, Damborg, P, Rosenkrantz, JT, Minga, UM, Mtambo, MMA & Olsen, JE 2018, 'First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania', Microbial Drug Resistance, vol. 24, no. 3, pp. 260-268. https://doi.org/10.1089/mdr.2016.0297

APA

Katakweba, A. A. S., Muhairwa, A. P., Lupindu, A. M., Damborg, P., Rosenkrantz, J. T., Minga, U. M., Mtambo, M. M. A., & Olsen, J. E. (2018). First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania. Microbial Drug Resistance, 24(3), 260-268. https://doi.org/10.1089/mdr.2016.0297

Vancouver

Katakweba AAS, Muhairwa AP, Lupindu AM, Damborg P, Rosenkrantz JT, Minga UM et al. First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania. Microbial Drug Resistance. 2018;24(3):260-268. https://doi.org/10.1089/mdr.2016.0297

Author

Katakweba, Abdul A.S. ; Muhairwa, Amandus P. ; Lupindu, Athumani M. ; Damborg, Peter ; Rosenkrantz, Jesper T. ; Minga, Uswege M. ; Mtambo, Madundo M.A. ; Olsen, John E. / First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania. In: Microbial Drug Resistance. 2018 ; Vol. 24, No. 3. pp. 260-268.

Bibtex

@article{7ce1310b81e54cb499bc5d80adce7c23,
title = "First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania",
abstract = "This study provides an estimate of antimicrobial resistance in intestinal indicator bacteria from humans (n = 97) and food animals (n = 388) in Tanzania. More than 70% of all fecal samples contained tetracycline (TE), sulfamethoxazole (STX), and ampicillin (AMP)-resistant coliforms, while cefotaxime (CTX)-resistant coliforms were observed in 40% of all samples. The average Log10 colony forming units/g of CTX-resistant coliforms in samples from humans were 2.20. Of 390 Escherichia coli tested, 66.4% were resistant to TE, 54.9% to STX, 54.9% to streptomycin, and 36.4% to CTX. Isolates were commonly (65.1%) multiresistant. All CTX-resistant isolates contained blaCTX-M gene type. AMP- and vancomycin-resistant enterococci were rare, and the average concentrations in positive samples were low (log10 0.9 and 0.4, respectively). A low-to-moderate resistance (2.1-15%) was detected in 240 enterococci isolates to the drugs tested, except for rifampicin resistance (75.2% of isolates). The average number of sulII gene copies varied between Log10 5.37 and 5.68 with no significant difference between sample source, while cattle had significantly higher number of tetW genes than humans. These findings, based on randomly obtained samples, will be instrumental in designing antimicrobial resistance (AMR) intervention strategies for Tanzania.",
keywords = "antimicrobial resistance, blactx-M, fecal indicator bacteria, Tanzania",
author = "Katakweba, {Abdul A.S.} and Muhairwa, {Amandus P.} and Lupindu, {Athumani M.} and Peter Damborg and Rosenkrantz, {Jesper T.} and Minga, {Uswege M.} and Mtambo, {Madundo M.A.} and Olsen, {John E.}",
year = "2018",
doi = "10.1089/mdr.2016.0297",
language = "English",
volume = "24",
pages = "260--268",
journal = "Microbial Drug Resistance",
issn = "1076-6294",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "3",

}

RIS

TY - JOUR

T1 - First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania

AU - Katakweba, Abdul A.S.

AU - Muhairwa, Amandus P.

AU - Lupindu, Athumani M.

AU - Damborg, Peter

AU - Rosenkrantz, Jesper T.

AU - Minga, Uswege M.

AU - Mtambo, Madundo M.A.

AU - Olsen, John E.

PY - 2018

Y1 - 2018

N2 - This study provides an estimate of antimicrobial resistance in intestinal indicator bacteria from humans (n = 97) and food animals (n = 388) in Tanzania. More than 70% of all fecal samples contained tetracycline (TE), sulfamethoxazole (STX), and ampicillin (AMP)-resistant coliforms, while cefotaxime (CTX)-resistant coliforms were observed in 40% of all samples. The average Log10 colony forming units/g of CTX-resistant coliforms in samples from humans were 2.20. Of 390 Escherichia coli tested, 66.4% were resistant to TE, 54.9% to STX, 54.9% to streptomycin, and 36.4% to CTX. Isolates were commonly (65.1%) multiresistant. All CTX-resistant isolates contained blaCTX-M gene type. AMP- and vancomycin-resistant enterococci were rare, and the average concentrations in positive samples were low (log10 0.9 and 0.4, respectively). A low-to-moderate resistance (2.1-15%) was detected in 240 enterococci isolates to the drugs tested, except for rifampicin resistance (75.2% of isolates). The average number of sulII gene copies varied between Log10 5.37 and 5.68 with no significant difference between sample source, while cattle had significantly higher number of tetW genes than humans. These findings, based on randomly obtained samples, will be instrumental in designing antimicrobial resistance (AMR) intervention strategies for Tanzania.

AB - This study provides an estimate of antimicrobial resistance in intestinal indicator bacteria from humans (n = 97) and food animals (n = 388) in Tanzania. More than 70% of all fecal samples contained tetracycline (TE), sulfamethoxazole (STX), and ampicillin (AMP)-resistant coliforms, while cefotaxime (CTX)-resistant coliforms were observed in 40% of all samples. The average Log10 colony forming units/g of CTX-resistant coliforms in samples from humans were 2.20. Of 390 Escherichia coli tested, 66.4% were resistant to TE, 54.9% to STX, 54.9% to streptomycin, and 36.4% to CTX. Isolates were commonly (65.1%) multiresistant. All CTX-resistant isolates contained blaCTX-M gene type. AMP- and vancomycin-resistant enterococci were rare, and the average concentrations in positive samples were low (log10 0.9 and 0.4, respectively). A low-to-moderate resistance (2.1-15%) was detected in 240 enterococci isolates to the drugs tested, except for rifampicin resistance (75.2% of isolates). The average number of sulII gene copies varied between Log10 5.37 and 5.68 with no significant difference between sample source, while cattle had significantly higher number of tetW genes than humans. These findings, based on randomly obtained samples, will be instrumental in designing antimicrobial resistance (AMR) intervention strategies for Tanzania.

KW - antimicrobial resistance

KW - blactx-M

KW - fecal indicator bacteria

KW - Tanzania

U2 - 10.1089/mdr.2016.0297

DO - 10.1089/mdr.2016.0297

M3 - Journal article

C2 - 28759321

AN - SCOPUS:85045444711

VL - 24

SP - 260

EP - 268

JO - Microbial Drug Resistance

JF - Microbial Drug Resistance

SN - 1076-6294

IS - 3

ER -

ID: 202029528