Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae

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Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae. / Bojer, Martin Saxtorph; Struve, Carsten; Ingmer, Hanne; Hansen, Dennis Schrøder; Krogfelt, Karen Angeliki.

In: P L o S One, Vol. 5, No. 11, 01.01.2010, p. e15467.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bojer, MS, Struve, C, Ingmer, H, Hansen, DS & Krogfelt, KA 2010, 'Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae', P L o S One, vol. 5, no. 11, pp. e15467. https://doi.org/10.1371/journal.pone.0015467

APA

Bojer, M. S., Struve, C., Ingmer, H., Hansen, D. S., & Krogfelt, K. A. (2010). Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae. P L o S One, 5(11), e15467. https://doi.org/10.1371/journal.pone.0015467

Vancouver

Bojer MS, Struve C, Ingmer H, Hansen DS, Krogfelt KA. Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae. P L o S One. 2010 Jan 1;5(11):e15467. https://doi.org/10.1371/journal.pone.0015467

Author

Bojer, Martin Saxtorph ; Struve, Carsten ; Ingmer, Hanne ; Hansen, Dennis Schrøder ; Krogfelt, Karen Angeliki. / Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae. In: P L o S One. 2010 ; Vol. 5, No. 11. pp. e15467.

Bibtex

@article{c7adf9bca17c40c284cc8eb35c0ce9ac,
title = "Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae",
abstract = "Klebsiella pneumoniae is an important opportunistic pathogen and a frequent cause of nosocomial infections. We have characterized a K. pneumoniae strain responsible for a series of critical infections in an intensive care unit over a two-year period. The strain was found to be remarkably thermotolerant providing a conceivable explanation of its persistence in the hospital environment. This marked phenotype is mediated by a novel type of Clp ATPase, designated ClpK. The clpK gene is encoded by a conjugative plasmid and we find that the clpK gene alone renders an otherwise sensitive E. coli strain resistant to lethal heat shock. Furthermore, one third of a collection of nosocomial K. pneumoniae isolates carry clpK and exhibit a heat resistant phenotype. The discovery of ClpK as a plasmid encoded factor and its profound impact on thermal stress survival sheds new light on the biological relevance of Clp ATPases in acquired environmental fitness and highlights the challenges of mobile genetic elements in fighting nosocomial infections.",
author = "Bojer, {Martin Saxtorph} and Carsten Struve and Hanne Ingmer and Hansen, {Dennis Schr{\o}der} and Krogfelt, {Karen Angeliki}",
year = "2010",
month = jan,
day = "1",
doi = "http://dx.doi.org/10.1371/journal.pone.0015467",
language = "English",
volume = "5",
pages = "e15467",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

RIS

TY - JOUR

T1 - Heat Resistance Mediated by a New Plasmid Encoded Clp ATPase, ClpK, as a Possible Novel Mechanism for Nosocomial Persistence of Klebsiella pneumoniae

AU - Bojer, Martin Saxtorph

AU - Struve, Carsten

AU - Ingmer, Hanne

AU - Hansen, Dennis Schrøder

AU - Krogfelt, Karen Angeliki

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Klebsiella pneumoniae is an important opportunistic pathogen and a frequent cause of nosocomial infections. We have characterized a K. pneumoniae strain responsible for a series of critical infections in an intensive care unit over a two-year period. The strain was found to be remarkably thermotolerant providing a conceivable explanation of its persistence in the hospital environment. This marked phenotype is mediated by a novel type of Clp ATPase, designated ClpK. The clpK gene is encoded by a conjugative plasmid and we find that the clpK gene alone renders an otherwise sensitive E. coli strain resistant to lethal heat shock. Furthermore, one third of a collection of nosocomial K. pneumoniae isolates carry clpK and exhibit a heat resistant phenotype. The discovery of ClpK as a plasmid encoded factor and its profound impact on thermal stress survival sheds new light on the biological relevance of Clp ATPases in acquired environmental fitness and highlights the challenges of mobile genetic elements in fighting nosocomial infections.

AB - Klebsiella pneumoniae is an important opportunistic pathogen and a frequent cause of nosocomial infections. We have characterized a K. pneumoniae strain responsible for a series of critical infections in an intensive care unit over a two-year period. The strain was found to be remarkably thermotolerant providing a conceivable explanation of its persistence in the hospital environment. This marked phenotype is mediated by a novel type of Clp ATPase, designated ClpK. The clpK gene is encoded by a conjugative plasmid and we find that the clpK gene alone renders an otherwise sensitive E. coli strain resistant to lethal heat shock. Furthermore, one third of a collection of nosocomial K. pneumoniae isolates carry clpK and exhibit a heat resistant phenotype. The discovery of ClpK as a plasmid encoded factor and its profound impact on thermal stress survival sheds new light on the biological relevance of Clp ATPases in acquired environmental fitness and highlights the challenges of mobile genetic elements in fighting nosocomial infections.

U2 - http://dx.doi.org/10.1371/journal.pone.0015467

DO - http://dx.doi.org/10.1371/journal.pone.0015467

M3 - Journal article

VL - 5

SP - e15467

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 11

ER -

ID: 34158569