How the RNA isolation method can affect microRNA microarray results
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How the RNA isolation method can affect microRNA microarray results. / Podolska, Agnieszka; Kaczkowski, Bogumil; Litman, Thomas; Fredholm, Merete; Cirera Salicio, Susanna.
In: Acta Biochimica Polonica, Vol. 58, No. 4, 2011, p. 535-540.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - How the RNA isolation method can affect microRNA microarray results
AU - Podolska, Agnieszka
AU - Kaczkowski, Bogumil
AU - Litman, Thomas
AU - Fredholm, Merete
AU - Cirera Salicio, Susanna
PY - 2011
Y1 - 2011
N2 - The quality of RNA is crucial in gene expression experiments. RNA degradation interferes in the measurement of gene expression, and in this context, microRNA quantification can lead to an incorrect estimation. In the present study, two different RNA isolation methods were used to perform microRNA microarray analysis on porcine brain tissue. One method is a phenol-guanidine isothiocyanate-based procedure that permits isolation of total RNA. The second method, miRVana™ microRNA isolation, is column based and recovers the small RNA fraction alone. We found that microarray analyses give different results that depend on the RNA fraction used, in particular because some microRNAs appear very sensitive to the RNA isolation method. We conclude that precautions need to be taken when comparing microarray studies based on RNA isolated with different methods.
AB - The quality of RNA is crucial in gene expression experiments. RNA degradation interferes in the measurement of gene expression, and in this context, microRNA quantification can lead to an incorrect estimation. In the present study, two different RNA isolation methods were used to perform microRNA microarray analysis on porcine brain tissue. One method is a phenol-guanidine isothiocyanate-based procedure that permits isolation of total RNA. The second method, miRVana™ microRNA isolation, is column based and recovers the small RNA fraction alone. We found that microarray analyses give different results that depend on the RNA fraction used, in particular because some microRNAs appear very sensitive to the RNA isolation method. We conclude that precautions need to be taken when comparing microarray studies based on RNA isolated with different methods.
KW - Animals
KW - Gene Expression Profiling
KW - MicroRNAs
KW - Oligonucleotide Array Sequence Analysis
KW - RNA
KW - RNA Stability
KW - Reagent Kits, Diagnostic
KW - Reproducibility of Results
KW - Sensitivity and Specificity
KW - Sequence Analysis, RNA
KW - Swine
M3 - Journal article
C2 - 22146134
VL - 58
SP - 535
EP - 540
JO - Acta Biochimica Polonica
JF - Acta Biochimica Polonica
SN - 0001-527X
IS - 4
ER -
ID: 119645509