Probes and polymerase chain reaction for detection of food-borne bacterial pathogens
Research output: Contribution to journal › Review › Research › peer-review
DNA-hybridization and the polymerase chain reaction (PCR) are techniques commonly used to detect pathogenic bacteria. In this paper, the use of these techniques for detection of Salmonella, E. coli, V. cholerae, non-O1 Vibrio, Yersinia enterocolitica, Campylobacter, Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, and C. botulinum is reviewed with emphasis on application in food microbiology. In food control, DNA-techniques have most often been used in a 'culture confirmation' fashion, i.e. bacteria are enriched and sometimes even purified by traditional culture procedures and thereafter identified by the use of DNA-based methods. The most desirable approach is, however, to detect organisms directly in the food, but major problems remain to be solved before this can be routinely performed.
Original language | English |
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Journal | International Journal of Food Microbiology |
Volume | 28 |
Issue number | 1 |
Pages (from-to) | 1-78 |
Number of pages | 78 |
ISSN | 0168-1605 |
DOIs | |
Publication status | Published - Nov 1995 |
- Foodborne, Oligonucleotides, Pathogens, PCR, Probes
Research areas
ID: 251185585