Occurrence of Pasteurellaceae bacteria in the oral cavity of the Tasmanian devil (Sarcophilus harrisii)
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Occurrence of Pasteurellaceae bacteria in the oral cavity of the Tasmanian devil (Sarcophilus harrisii). / Brix, Lena; Hansen, Mie Johanne; Kelly, Androo; Bertelsen, Mads Frost; Bojesen, Anders Miki.
In: Journal of Zoo and Wildlife Medicine, Vol. 46, No. 2, 06.2015, p. 241-245.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Occurrence of Pasteurellaceae bacteria in the oral cavity of the Tasmanian devil (Sarcophilus harrisii)
AU - Brix, Lena
AU - Hansen, Mie Johanne
AU - Kelly, Androo
AU - Bertelsen, Mads Frost
AU - Bojesen, Anders Miki
PY - 2015/6
Y1 - 2015/6
N2 - The occurrence of bacteria belonging to the family Pasteurellaceae in the oral cavity of captive Tasmanian devils (Sarcophilus harrisii) was investigated using phenotypic and subsequent genotypic characterization and phylogenetic analyses. A total of 62 bacterial isolates obtained from Tasmanian devils, tentatively classified with the family Pasteurellaceae, were further characterized by phylogenetic analysis of rpoB gene sequence similarity, which showed that the isolates investigated formed five distinct groups. A total of 15 strains formed a novel genus-like group within Pasteurellaceae. Thirty-six strains grouped with the type strain of Frederiksenia canicola. Five strains clustered with the type strain of Pasteurella multocida . Interestingly, four of the P. multocida-like strains were β-hemolytic when incubated on blood agar, which is atypical for this genus. Five strains grouped with a 100% rpoB similarity with Pasteurella dagmatis. Finally, a single strain showed 97.1% resemblance to Haemophilus haemoglobinophilus. The results demonstrate that Tasmanian devils are hosting a variety of bacterial taxa affiliated with the family of Pasteurellaceae as part of their oral microflora.
AB - The occurrence of bacteria belonging to the family Pasteurellaceae in the oral cavity of captive Tasmanian devils (Sarcophilus harrisii) was investigated using phenotypic and subsequent genotypic characterization and phylogenetic analyses. A total of 62 bacterial isolates obtained from Tasmanian devils, tentatively classified with the family Pasteurellaceae, were further characterized by phylogenetic analysis of rpoB gene sequence similarity, which showed that the isolates investigated formed five distinct groups. A total of 15 strains formed a novel genus-like group within Pasteurellaceae. Thirty-six strains grouped with the type strain of Frederiksenia canicola. Five strains clustered with the type strain of Pasteurella multocida . Interestingly, four of the P. multocida-like strains were β-hemolytic when incubated on blood agar, which is atypical for this genus. Five strains grouped with a 100% rpoB similarity with Pasteurella dagmatis. Finally, a single strain showed 97.1% resemblance to Haemophilus haemoglobinophilus. The results demonstrate that Tasmanian devils are hosting a variety of bacterial taxa affiliated with the family of Pasteurellaceae as part of their oral microflora.
KW - Animals
KW - Animals, Zoo
KW - Marsupialia
KW - Mouth
KW - Pasteurellaceae
KW - Phylogeny
KW - Frederiksenia
KW - marsupials
KW - Pasteurella
KW - Tasmanian devil
KW - Sarcophilus harrisii
U2 - 10.1638/2014-0111R1.1
DO - 10.1638/2014-0111R1.1
M3 - Journal article
C2 - 26056874
VL - 46
SP - 241
EP - 245
JO - Journal of Zoo and Wildlife Medicine
JF - Journal of Zoo and Wildlife Medicine
SN - 1042-7260
IS - 2
ER -
ID: 144542926