The distribution, number, and certain neurochemical identities of infracortical white matter neurons in the brains of a southern lesser galago, a black-capped squirrel monkey, and a crested macaque
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The distribution, number, and certain neurochemical identities of infracortical white matter neurons in the brains of a southern lesser galago, a black-capped squirrel monkey, and a crested macaque. / Swiegers, Jordan; Bhagwandin, Adhil; Maseko, Busisiwe C.; Sherwood, Chet C.; Hård, Therese; Bertelsen, Mads F.; Spocter, Muhammad A.; Molnár, Zoltán; Manger, Paul R.
In: Journal of Comparative Neurology, Vol. 529, No. 16, 2021, p. 3676-3708.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The distribution, number, and certain neurochemical identities of infracortical white matter neurons in the brains of a southern lesser galago, a black-capped squirrel monkey, and a crested macaque
AU - Swiegers, Jordan
AU - Bhagwandin, Adhil
AU - Maseko, Busisiwe C.
AU - Sherwood, Chet C.
AU - Hård, Therese
AU - Bertelsen, Mads F.
AU - Spocter, Muhammad A.
AU - Molnár, Zoltán
AU - Manger, Paul R.
N1 - Publisher Copyright: © 2021 Wiley Periodicals LLC
PY - 2021
Y1 - 2021
N2 - In the current study, we examined the number, distribution, and aspects of the neurochemical identities of infracortical white matter neurons, also termed white matter interstitial cells (WMICs), in the brains of a southern lesser galago (Galago moholi), a black-capped squirrel monkey (Saimiri boliviensis boliviensis), and a crested macaque (Macaca nigra). Staining for neuronal nuclear marker (NeuN) revealed WMICs throughout the infracortical white matter, these cells being most dense close to inner cortical border, decreasing in density with depth in the white matter. Stereological analysis of NeuN-immunopositive cells revealed estimates of approximately 1.1, 10.8, and 37.7 million WMICs within the infracortical white matter of the galago, squirrel monkey, and crested macaque, respectively. The total numbers of WMICs form a distinct negative allometric relationship with brain mass and white matter volume when examined in a larger sample of primates where similar measures have been obtained. In all three primates studied, the highest densities of WMICs were in the white matter of the frontal lobe, with the occipital lobe having the lowest. Immunostaining revealed significant subpopulations of WMICs containing neuronal nitric oxide synthase (nNOS) and calretinin, with very few WMICs containing parvalbumin, and none containing calbindin. The nNOS and calretinin immunopositive WMICs represent approximately 21% of the total WMIC population; however, variances in the proportions of these neurochemical phenotypes were noted. Our results indicate that both the squirrel monkey and crested macaque might be informative animal models for the study of WMICs in neurodegenerative and psychiatric disorders in humans.
AB - In the current study, we examined the number, distribution, and aspects of the neurochemical identities of infracortical white matter neurons, also termed white matter interstitial cells (WMICs), in the brains of a southern lesser galago (Galago moholi), a black-capped squirrel monkey (Saimiri boliviensis boliviensis), and a crested macaque (Macaca nigra). Staining for neuronal nuclear marker (NeuN) revealed WMICs throughout the infracortical white matter, these cells being most dense close to inner cortical border, decreasing in density with depth in the white matter. Stereological analysis of NeuN-immunopositive cells revealed estimates of approximately 1.1, 10.8, and 37.7 million WMICs within the infracortical white matter of the galago, squirrel monkey, and crested macaque, respectively. The total numbers of WMICs form a distinct negative allometric relationship with brain mass and white matter volume when examined in a larger sample of primates where similar measures have been obtained. In all three primates studied, the highest densities of WMICs were in the white matter of the frontal lobe, with the occipital lobe having the lowest. Immunostaining revealed significant subpopulations of WMICs containing neuronal nitric oxide synthase (nNOS) and calretinin, with very few WMICs containing parvalbumin, and none containing calbindin. The nNOS and calretinin immunopositive WMICs represent approximately 21% of the total WMIC population; however, variances in the proportions of these neurochemical phenotypes were noted. Our results indicate that both the squirrel monkey and crested macaque might be informative animal models for the study of WMICs in neurodegenerative and psychiatric disorders in humans.
KW - cortical evolution
KW - immunohistochemistry
KW - neurochemistry
KW - primate brain
KW - RRID AB_10000321
KW - RRID AB_10000340
KW - RRID AB_10000343
KW - RRID AB_11204707
KW - RRID AB_91824
KW - white matter neurons
U2 - 10.1002/cne.25216
DO - 10.1002/cne.25216
M3 - Journal article
C2 - 34259349
AN - SCOPUS:85110982333
VL - 529
SP - 3676
EP - 3708
JO - The Journal of Comparative Neurology
JF - The Journal of Comparative Neurology
SN - 0021-9967
IS - 16
ER -
ID: 282940964