A hybrid baculovirus-bacteriophage T7 transient expression system

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A hybrid baculovirus-bacteriophage T7 transient expression system. / Van Poelwijk, Frank; Broer, René; Belsham, Graham J.; Oudshoom, Peter; Vlak, Just M.; Goldbach, Rob W.

In: Bio/Technology, Vol. 13, No. 3, 03.1995, p. 261-264.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Van Poelwijk, F, Broer, R, Belsham, GJ, Oudshoom, P, Vlak, JM & Goldbach, RW 1995, 'A hybrid baculovirus-bacteriophage T7 transient expression system', Bio/Technology, vol. 13, no. 3, pp. 261-264. https://doi.org/10.1038/nbt0395-261

APA

Van Poelwijk, F., Broer, R., Belsham, G. J., Oudshoom, P., Vlak, J. M., & Goldbach, R. W. (1995). A hybrid baculovirus-bacteriophage T7 transient expression system. Bio/Technology, 13(3), 261-264. https://doi.org/10.1038/nbt0395-261

Vancouver

Van Poelwijk F, Broer R, Belsham GJ, Oudshoom P, Vlak JM, Goldbach RW. A hybrid baculovirus-bacteriophage T7 transient expression system. Bio/Technology. 1995 Mar;13(3):261-264. https://doi.org/10.1038/nbt0395-261

Author

Van Poelwijk, Frank ; Broer, René ; Belsham, Graham J. ; Oudshoom, Peter ; Vlak, Just M. ; Goldbach, Rob W. / A hybrid baculovirus-bacteriophage T7 transient expression system. In: Bio/Technology. 1995 ; Vol. 13, No. 3. pp. 261-264.

Bibtex

@article{b5a7293f164a4d00bc10c4187d1ae543,
title = "A hybrid baculovirus-bacteriophage T7 transient expression system",
abstract = "A hybrid recombinant baculovirus-bacteriophage T7 expression system was developed for transient expression in insect cells of plasmids with foreign genes provided with a 17 promoter. The coding sequence for 17 RNA polymerase, with or without a nuclear localization signal, was inserted into the genome of Autographa californica nuclear polyhedrosis virus. Recombinant viruses stably expressed 17 RNA polymerase in insect cells. Upon transfection of infected insect cells with plasmids containing the genes for chloramphenicol acetyltransferase (CAT), the hepatitis B virus precore-, core- or e-antigens under control of the T7 promoter, transient expression of these genes was detected by ELISA. The results obtained indicate that this baculovirus/T7 system provides a simple and widely applicable tool for transient gene expression studies.",
author = "{Van Poelwijk}, Frank and Ren{\'e} Broer and Belsham, {Graham J.} and Peter Oudshoom and Vlak, {Just M.} and Goldbach, {Rob W.}",
year = "1995",
month = mar,
doi = "10.1038/nbt0395-261",
language = "English",
volume = "13",
pages = "261--264",
journal = "Bio/Technology",
issn = "0015-2684",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - A hybrid baculovirus-bacteriophage T7 transient expression system

AU - Van Poelwijk, Frank

AU - Broer, René

AU - Belsham, Graham J.

AU - Oudshoom, Peter

AU - Vlak, Just M.

AU - Goldbach, Rob W.

PY - 1995/3

Y1 - 1995/3

N2 - A hybrid recombinant baculovirus-bacteriophage T7 expression system was developed for transient expression in insect cells of plasmids with foreign genes provided with a 17 promoter. The coding sequence for 17 RNA polymerase, with or without a nuclear localization signal, was inserted into the genome of Autographa californica nuclear polyhedrosis virus. Recombinant viruses stably expressed 17 RNA polymerase in insect cells. Upon transfection of infected insect cells with plasmids containing the genes for chloramphenicol acetyltransferase (CAT), the hepatitis B virus precore-, core- or e-antigens under control of the T7 promoter, transient expression of these genes was detected by ELISA. The results obtained indicate that this baculovirus/T7 system provides a simple and widely applicable tool for transient gene expression studies.

AB - A hybrid recombinant baculovirus-bacteriophage T7 expression system was developed for transient expression in insect cells of plasmids with foreign genes provided with a 17 promoter. The coding sequence for 17 RNA polymerase, with or without a nuclear localization signal, was inserted into the genome of Autographa californica nuclear polyhedrosis virus. Recombinant viruses stably expressed 17 RNA polymerase in insect cells. Upon transfection of infected insect cells with plasmids containing the genes for chloramphenicol acetyltransferase (CAT), the hepatitis B virus precore-, core- or e-antigens under control of the T7 promoter, transient expression of these genes was detected by ELISA. The results obtained indicate that this baculovirus/T7 system provides a simple and widely applicable tool for transient gene expression studies.

UR - http://www.scopus.com/inward/record.url?scp=0028961409&partnerID=8YFLogxK

U2 - 10.1038/nbt0395-261

DO - 10.1038/nbt0395-261

M3 - Journal article

C2 - 9634768

AN - SCOPUS:0028961409

VL - 13

SP - 261

EP - 264

JO - Bio/Technology

JF - Bio/Technology

SN - 0015-2684

IS - 3

ER -

ID: 379029163