Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae. / BELSHAM, Graham J.; BARKER, David G.; SMITH, Alan E.

In: European Journal of Biochemistry, Vol. 156, No. 2, 04.1986, p. 413-421.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

BELSHAM, GJ, BARKER, DG & SMITH, AE 1986, 'Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae', European Journal of Biochemistry, vol. 156, no. 2, pp. 413-421. https://doi.org/10.1111/j.1432-1033.1986.tb09598.x

APA

BELSHAM, G. J., BARKER, D. G., & SMITH, A. E. (1986). Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae. European Journal of Biochemistry, 156(2), 413-421. https://doi.org/10.1111/j.1432-1033.1986.tb09598.x

Vancouver

BELSHAM GJ, BARKER DG, SMITH AE. Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae. European Journal of Biochemistry. 1986 Apr;156(2):413-421. https://doi.org/10.1111/j.1432-1033.1986.tb09598.x

Author

BELSHAM, Graham J. ; BARKER, David G. ; SMITH, Alan E. / Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae. In: European Journal of Biochemistry. 1986 ; Vol. 156, No. 2. pp. 413-421.

Bibtex

@article{b76318be7aa84b079d66ce4100de3742,
title = "Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae",
abstract = "The polyoma middle‐T gene, lacking its intron, was inserted into a yeast expression plasmid containing the phosphoglycerate kinase promoter. Such plasmids transformed yeast at low frequency and these transformants expressed middle‐T antigen at a level of approximately 0.1% cell protein. Furthermore, expression of this protein was frequently lost during growth in liquid culture and this loss of middle‐T was accompanied by a twofold increase in the rate of growth. The spontaneous production of a truncated middle‐T antigen, lacking the C terminus, was also observed; the expression of this protein did not inhibit the growth rate of the cells. Recovery and analysis of the expression plasmids encoding the truncated molecule showed that a single C · G base pair had been deleted from a run of nine consecutive C · G base pairs (Pyr nucleotide 1239–1247) within the middle‐T coding region. This frame‐shift mutation results in premature termination of the protein and loss of the strongly hydrophobic region of the molecule believed to be responsible for the membrane association of middle‐T antigen.",
author = "BELSHAM, {Graham J.} and BARKER, {David G.} and SMITH, {Alan E.}",
year = "1986",
month = apr,
doi = "10.1111/j.1432-1033.1986.tb09598.x",
language = "English",
volume = "156",
pages = "413--421",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Springer Verlag",
number = "2",

}

RIS

TY - JOUR

T1 - Expression of polyoma virus middle‐T antigen in Saccharomyces cerevisiae

AU - BELSHAM, Graham J.

AU - BARKER, David G.

AU - SMITH, Alan E.

PY - 1986/4

Y1 - 1986/4

N2 - The polyoma middle‐T gene, lacking its intron, was inserted into a yeast expression plasmid containing the phosphoglycerate kinase promoter. Such plasmids transformed yeast at low frequency and these transformants expressed middle‐T antigen at a level of approximately 0.1% cell protein. Furthermore, expression of this protein was frequently lost during growth in liquid culture and this loss of middle‐T was accompanied by a twofold increase in the rate of growth. The spontaneous production of a truncated middle‐T antigen, lacking the C terminus, was also observed; the expression of this protein did not inhibit the growth rate of the cells. Recovery and analysis of the expression plasmids encoding the truncated molecule showed that a single C · G base pair had been deleted from a run of nine consecutive C · G base pairs (Pyr nucleotide 1239–1247) within the middle‐T coding region. This frame‐shift mutation results in premature termination of the protein and loss of the strongly hydrophobic region of the molecule believed to be responsible for the membrane association of middle‐T antigen.

AB - The polyoma middle‐T gene, lacking its intron, was inserted into a yeast expression plasmid containing the phosphoglycerate kinase promoter. Such plasmids transformed yeast at low frequency and these transformants expressed middle‐T antigen at a level of approximately 0.1% cell protein. Furthermore, expression of this protein was frequently lost during growth in liquid culture and this loss of middle‐T was accompanied by a twofold increase in the rate of growth. The spontaneous production of a truncated middle‐T antigen, lacking the C terminus, was also observed; the expression of this protein did not inhibit the growth rate of the cells. Recovery and analysis of the expression plasmids encoding the truncated molecule showed that a single C · G base pair had been deleted from a run of nine consecutive C · G base pairs (Pyr nucleotide 1239–1247) within the middle‐T coding region. This frame‐shift mutation results in premature termination of the protein and loss of the strongly hydrophobic region of the molecule believed to be responsible for the membrane association of middle‐T antigen.

UR - http://www.scopus.com/inward/record.url?scp=0023049452&partnerID=8YFLogxK

U2 - 10.1111/j.1432-1033.1986.tb09598.x

DO - 10.1111/j.1432-1033.1986.tb09598.x

M3 - Journal article

C2 - 3009184

AN - SCOPUS:0023049452

VL - 156

SP - 413

EP - 421

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 2

ER -

ID: 382371037