Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis. / Percy, Neil; Belsham, Graham J.; Brangwyn, Julia K.; Sullivan, Michael; Stone, David M.; Almond, Jeffrey W.

In: Journal of Virology, Vol. 66, No. 3, 03.1992, p. 1695-1701.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Percy, N, Belsham, GJ, Brangwyn, JK, Sullivan, M, Stone, DM & Almond, JW 1992, 'Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis', Journal of Virology, vol. 66, no. 3, pp. 1695-1701.

APA

Percy, N., Belsham, G. J., Brangwyn, J. K., Sullivan, M., Stone, D. M., & Almond, J. W. (1992). Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis. Journal of Virology, 66(3), 1695-1701.

Vancouver

Percy N, Belsham GJ, Brangwyn JK, Sullivan M, Stone DM, Almond JW. Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis. Journal of Virology. 1992 Mar;66(3):1695-1701.

Author

Percy, Neil ; Belsham, Graham J. ; Brangwyn, Julia K. ; Sullivan, Michael ; Stone, David M. ; Almond, Jeffrey W. / Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis. In: Journal of Virology. 1992 ; Vol. 66, No. 3. pp. 1695-1701.

Bibtex

@article{84fa065f075f44f4b37abe4e0561e065,
title = "Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis",
abstract = "A series of genetic deletions based partly on two RNA secondary structure models (M. A. Skinner, V. R. Racaniello, G. Dunn, J. Cooper, P. D. Minor, and J. W. Almond, J. Mol. Biol. 207:379-392, 1989; E. V. Pilipenko, V. M. Blinov, L. I. Romanova, A. N. Sinyakov, S. V. Maslova, and V. I. Agol, Virology 168:201-209, 1989) was made in the cDNA encoding the 5′ noncoding region (5′ NCR) of the poliovirus genome in order to study the sequences that direct the internal entry of ribosomes. The modified cDNAs were placed between two open reading frames in a single transcriptional unit and used to transfect cells in culture. Internal entry of ribosomes was detected by measuring translation from the second open reading frame in the bicistronic mRNA. When assayed alone, a large proportion of the poliovirus 5′ NCR superstructure including several well-defined stem-loops was required for ribosome entry and efficient translation. However, in cells cotransfected with a complete infectious poliovirus cDNA, the requirement for the stem-loops in this large superstructure was reduced. The results suggest that virus infection modifies the cellular translational machinery, so that shortened forms of the 5′ NCR are sufficient for cap-independent translation, and that the internal entry of ribosomes occurs by two distinct modes during the virus replication cycle.",
author = "Neil Percy and Belsham, {Graham J.} and Brangwyn, {Julia K.} and Michael Sullivan and Stone, {David M.} and Almond, {Jeffrey W.}",
year = "1992",
month = mar,
language = "English",
volume = "66",
pages = "1695--1701",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "3",

}

RIS

TY - JOUR

T1 - Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5′ noncoding region of the genome involved in internal initiation of protein synthesis

AU - Percy, Neil

AU - Belsham, Graham J.

AU - Brangwyn, Julia K.

AU - Sullivan, Michael

AU - Stone, David M.

AU - Almond, Jeffrey W.

PY - 1992/3

Y1 - 1992/3

N2 - A series of genetic deletions based partly on two RNA secondary structure models (M. A. Skinner, V. R. Racaniello, G. Dunn, J. Cooper, P. D. Minor, and J. W. Almond, J. Mol. Biol. 207:379-392, 1989; E. V. Pilipenko, V. M. Blinov, L. I. Romanova, A. N. Sinyakov, S. V. Maslova, and V. I. Agol, Virology 168:201-209, 1989) was made in the cDNA encoding the 5′ noncoding region (5′ NCR) of the poliovirus genome in order to study the sequences that direct the internal entry of ribosomes. The modified cDNAs were placed between two open reading frames in a single transcriptional unit and used to transfect cells in culture. Internal entry of ribosomes was detected by measuring translation from the second open reading frame in the bicistronic mRNA. When assayed alone, a large proportion of the poliovirus 5′ NCR superstructure including several well-defined stem-loops was required for ribosome entry and efficient translation. However, in cells cotransfected with a complete infectious poliovirus cDNA, the requirement for the stem-loops in this large superstructure was reduced. The results suggest that virus infection modifies the cellular translational machinery, so that shortened forms of the 5′ NCR are sufficient for cap-independent translation, and that the internal entry of ribosomes occurs by two distinct modes during the virus replication cycle.

AB - A series of genetic deletions based partly on two RNA secondary structure models (M. A. Skinner, V. R. Racaniello, G. Dunn, J. Cooper, P. D. Minor, and J. W. Almond, J. Mol. Biol. 207:379-392, 1989; E. V. Pilipenko, V. M. Blinov, L. I. Romanova, A. N. Sinyakov, S. V. Maslova, and V. I. Agol, Virology 168:201-209, 1989) was made in the cDNA encoding the 5′ noncoding region (5′ NCR) of the poliovirus genome in order to study the sequences that direct the internal entry of ribosomes. The modified cDNAs were placed between two open reading frames in a single transcriptional unit and used to transfect cells in culture. Internal entry of ribosomes was detected by measuring translation from the second open reading frame in the bicistronic mRNA. When assayed alone, a large proportion of the poliovirus 5′ NCR superstructure including several well-defined stem-loops was required for ribosome entry and efficient translation. However, in cells cotransfected with a complete infectious poliovirus cDNA, the requirement for the stem-loops in this large superstructure was reduced. The results suggest that virus infection modifies the cellular translational machinery, so that shortened forms of the 5′ NCR are sufficient for cap-independent translation, and that the internal entry of ribosomes occurs by two distinct modes during the virus replication cycle.

UR - http://www.scopus.com/inward/record.url?scp=0026561505&partnerID=8YFLogxK

M3 - Journal article

C2 - 1310772

AN - SCOPUS:0026561505

VL - 66

SP - 1695

EP - 1701

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 3

ER -

ID: 381222724