A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink

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A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink. / Cirera Salicio, Susanna; Markakis, Marios Nektarios; Kristiansen, Thea; Vissenberg, Kris; Fredholm, Merete; Christensen, Knud Arnbjerg; Anistoroaei, Razvan Marian.

In: Mammalian Genome, Vol. 27, No. 3, 04.2016, p. 135-143.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cirera Salicio, S, Markakis, MN, Kristiansen, T, Vissenberg, K, Fredholm, M, Christensen, KA & Anistoroaei, RM 2016, 'A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink', Mammalian Genome, vol. 27, no. 3, pp. 135-143. https://doi.org/10.1007/s00335-016-9620-4

APA

Cirera Salicio, S., Markakis, M. N., Kristiansen, T., Vissenberg, K., Fredholm, M., Christensen, K. A., & Anistoroaei, R. M. (2016). A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink. Mammalian Genome, 27(3), 135-143. https://doi.org/10.1007/s00335-016-9620-4

Vancouver

Cirera Salicio S, Markakis MN, Kristiansen T, Vissenberg K, Fredholm M, Christensen KA et al. A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink. Mammalian Genome. 2016 Apr;27(3):135-143. https://doi.org/10.1007/s00335-016-9620-4

Author

Cirera Salicio, Susanna ; Markakis, Marios Nektarios ; Kristiansen, Thea ; Vissenberg, Kris ; Fredholm, Merete ; Christensen, Knud Arnbjerg ; Anistoroaei, Razvan Marian. / A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink. In: Mammalian Genome. 2016 ; Vol. 27, No. 3. pp. 135-143.

Bibtex

@article{1d3b97ea727d4fadb4022d8ba37bd81a,
title = "A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink",
abstract = "A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b (P) b (P) ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials for amplifying the genomic DNA and cDNA at the beginning of intron 2 of AP TYRP1 revealed the presence of a large insertion of approximately eight kb. The insertion most likely disrupts different elements necessary for the splicing of intron 2 of the TYRP1 gene. In AP RNAseq data indicate, however, the presence of the wild-type (wt) transcript at very low levels and Western blot reveals three products when using an antibody raised against middle part of the TYRP1 protein. One individual from another brown mink phenotype-commercially named Dawn-was also investigated at the molecular level by long-range PCR and the same size insertion appears to be present. By this we suggest that certain modifiers of TYRP1 would induce different brown colour degradation, which results in at least two different phases of brown.",
author = "{Cirera Salicio}, Susanna and Markakis, {Marios Nektarios} and Thea Kristiansen and Kris Vissenberg and Merete Fredholm and Christensen, {Knud Arnbjerg} and Anistoroaei, {Razvan Marian}",
year = "2016",
month = apr,
doi = "10.1007/s00335-016-9620-4",
language = "English",
volume = "27",
pages = "135--143",
journal = "Mammalian Genome",
issn = "0938-8990",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - A large insertion in intron 2 of the TYRP1 gene associated with American Palomino phenotype in American mink

AU - Cirera Salicio, Susanna

AU - Markakis, Marios Nektarios

AU - Kristiansen, Thea

AU - Vissenberg, Kris

AU - Fredholm, Merete

AU - Christensen, Knud Arnbjerg

AU - Anistoroaei, Razvan Marian

PY - 2016/4

Y1 - 2016/4

N2 - A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b (P) b (P) ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials for amplifying the genomic DNA and cDNA at the beginning of intron 2 of AP TYRP1 revealed the presence of a large insertion of approximately eight kb. The insertion most likely disrupts different elements necessary for the splicing of intron 2 of the TYRP1 gene. In AP RNAseq data indicate, however, the presence of the wild-type (wt) transcript at very low levels and Western blot reveals three products when using an antibody raised against middle part of the TYRP1 protein. One individual from another brown mink phenotype-commercially named Dawn-was also investigated at the molecular level by long-range PCR and the same size insertion appears to be present. By this we suggest that certain modifiers of TYRP1 would induce different brown colour degradation, which results in at least two different phases of brown.

AB - A number of American mink phenotypes display a range of brownish colours. One of these phenotypes, namely American Palomino (b (P) b (P) ) (AP) has been found to be associated with the tyrosinase-related protein 1 (TYRP1) gene by genotyping microsatellite markers in one sire family. Trials for amplifying the genomic DNA and cDNA at the beginning of intron 2 of AP TYRP1 revealed the presence of a large insertion of approximately eight kb. The insertion most likely disrupts different elements necessary for the splicing of intron 2 of the TYRP1 gene. In AP RNAseq data indicate, however, the presence of the wild-type (wt) transcript at very low levels and Western blot reveals three products when using an antibody raised against middle part of the TYRP1 protein. One individual from another brown mink phenotype-commercially named Dawn-was also investigated at the molecular level by long-range PCR and the same size insertion appears to be present. By this we suggest that certain modifiers of TYRP1 would induce different brown colour degradation, which results in at least two different phases of brown.

U2 - 10.1007/s00335-016-9620-4

DO - 10.1007/s00335-016-9620-4

M3 - Journal article

C2 - 26886941

VL - 27

SP - 135

EP - 143

JO - Mammalian Genome

JF - Mammalian Genome

SN - 0938-8990

IS - 3

ER -

ID: 160667958