A new furunculosis challenge method for evaluation of vaccine efficacy in rainbow trout
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A new furunculosis challenge method for evaluation of vaccine efficacy in rainbow trout. / Marana, Moonika Haahr; Chettri, Jiwan Kumar; Skov, Jakob; Dalsgaard, Inger; Kania, Per Walter; Buchmann, Kurt.
In: Open Journal of Immunology, Vol. 6, 28.11.2016, p. 136-147.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A new furunculosis challenge method for evaluation of vaccine efficacy in rainbow trout
AU - Marana, Moonika Haahr
AU - Chettri, Jiwan Kumar
AU - Skov, Jakob
AU - Dalsgaard, Inger
AU - Kania, Per Walter
AU - Buchmann, Kurt
PY - 2016/11/28
Y1 - 2016/11/28
N2 - Experimental infection of fish for vaccine efficacy studies is associated with several limitations. Administration of live bacteria with the purpose of causing disease in fish can be performed by co-habitation, immersion or injection. We have developed a new Aeromonas salmonicida challenge method for rainbow trout and have applied it for evaluation of furunculosis vaccine efficacy. The method reveals development of systemic immunity in fish as live bacteria are introduced in the tail fin epidermis distant from the vaccine injection site (peritoneal cavity). This method seeks to mimic natural infection in fish farms where tail biting and therefore bacterial expo- sure to tail fin ulcers is widespread. By use of a multi-needle device ten epidermal perforations were introduced in the dorsal part of the tail fin of anaesthetized rainbow trout (vaccinated or naive). Subsequently 100 μL (3.4 × 108 colony-forming units (CFU) mL−1) of a 48 hour culture of Aeromonas salmonicida subsp. salmo- nicida strain 090710-1/23 was placed at the perforation site for 60 sec whereafter fish were allowed to regain consciousness in clean freshwater. Immunohistochemistry and scanning electron microscopy illustrated the spread of bacteria from the injection site. Classical furunculosis symptoms associated with a high morbidity rate were observed in control fish whereas vaccinated fish exhibited a significantly higher survival.
AB - Experimental infection of fish for vaccine efficacy studies is associated with several limitations. Administration of live bacteria with the purpose of causing disease in fish can be performed by co-habitation, immersion or injection. We have developed a new Aeromonas salmonicida challenge method for rainbow trout and have applied it for evaluation of furunculosis vaccine efficacy. The method reveals development of systemic immunity in fish as live bacteria are introduced in the tail fin epidermis distant from the vaccine injection site (peritoneal cavity). This method seeks to mimic natural infection in fish farms where tail biting and therefore bacterial expo- sure to tail fin ulcers is widespread. By use of a multi-needle device ten epidermal perforations were introduced in the dorsal part of the tail fin of anaesthetized rainbow trout (vaccinated or naive). Subsequently 100 μL (3.4 × 108 colony-forming units (CFU) mL−1) of a 48 hour culture of Aeromonas salmonicida subsp. salmo- nicida strain 090710-1/23 was placed at the perforation site for 60 sec whereafter fish were allowed to regain consciousness in clean freshwater. Immunohistochemistry and scanning electron microscopy illustrated the spread of bacteria from the injection site. Classical furunculosis symptoms associated with a high morbidity rate were observed in control fish whereas vaccinated fish exhibited a significantly higher survival.
U2 - 10.4236/oji.2016.64014
DO - 10.4236/oji.2016.64014
M3 - Journal article
VL - 6
SP - 136
EP - 147
JO - Open Journal of Immunology
JF - Open Journal of Immunology
SN - 2162-450X
ER -
ID: 169705150