A Rapid Bacteriophage DNA Extraction Method
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A Rapid Bacteriophage DNA Extraction Method. / Jakociune, Dziuginta; Moodley, Arshnee.
In: Biology Methods and Protocols, Vol. 1, No. 3, 27, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A Rapid Bacteriophage DNA Extraction Method
AU - Jakociune, Dziuginta
AU - Moodley, Arshnee
PY - 2018
Y1 - 2018
N2 - Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform
AB - Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform
U2 - 10.3390/mps1030027
DO - 10.3390/mps1030027
M3 - Journal article
C2 - 31164569
VL - 1
JO - Biology Methods and Protocols
JF - Biology Methods and Protocols
SN - 2396-8923
IS - 3
M1 - 27
ER -
ID: 200138545