A ring test of a wireless in vitro gas production system
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A ring test of a wireless in vitro gas production system. / Cornou, Cecile; Storm, Ida Marie Lindhardt Drejer; Hindrichsen, Ida Katarina Auf der M. ; Worgan, Hilary; Bakewell, Eleanor; Ruiz, David R. Yáñez; Abecia, Leticia; Tagliapietra, Franco; Cattani, Mirko; Ritz, Christian; Hansen, Hanne Helene.
In: Animal Production Science, Vol. 53, No. 6, 2013, p. 585-592.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A ring test of a wireless in vitro gas production system
AU - Cornou, Cecile
AU - Storm, Ida Marie Lindhardt Drejer
AU - Hindrichsen, Ida Katarina Auf der M.
AU - Worgan, Hilary
AU - Bakewell, Eleanor
AU - Ruiz, David R. Yáñez
AU - Abecia, Leticia
AU - Tagliapietra, Franco
AU - Cattani, Mirko
AU - Ritz, Christian
AU - Hansen, Hanne Helene
PY - 2013
Y1 - 2013
N2 - The in vitro gas production (GP) technique has been widely used for feed evaluation. However, variability in results limits useful comparisons. Results from a ring test undertaken in four laboratories (Italy - IT, Spain - SP, Wales - WA and Denmark - DK) using the same wireless equipment (ANKOM Technology), same substrates and same laboratory protocol are presented, including calculation of repeatability and reproducibility according to ISO 5725-2. Hay, maize starch and straw samples and units without sample (blanks) were incubated in five repetitions using rumen inoculum from cows (DK, IT and WA) or sheep (SP). Curves, corrected for blanks, were fitted using an exponential regression model with a lag time. The following variables were considered: (i) GP24 and GP48: raw values at 24 and 48 h (mL/g DM), corrected for blanks; (ii) A: asymptotic GP (mL/g DM); (iii) T1/2: time when half A is produced (h); (iv) GPMR: maximum predicted GP rate (mL/h); (v) L: lag time (h). A mixed model including laboratories as random effect was used. A significant interaction between substrate and laboratories was found for all variables except A. The most repeatable and reproducible results were observed for A and GP48. The results from this ring test suggest the need for more standardisation, particularly in the procedures that occur outside the laboratory.
AB - The in vitro gas production (GP) technique has been widely used for feed evaluation. However, variability in results limits useful comparisons. Results from a ring test undertaken in four laboratories (Italy - IT, Spain - SP, Wales - WA and Denmark - DK) using the same wireless equipment (ANKOM Technology), same substrates and same laboratory protocol are presented, including calculation of repeatability and reproducibility according to ISO 5725-2. Hay, maize starch and straw samples and units without sample (blanks) were incubated in five repetitions using rumen inoculum from cows (DK, IT and WA) or sheep (SP). Curves, corrected for blanks, were fitted using an exponential regression model with a lag time. The following variables were considered: (i) GP24 and GP48: raw values at 24 and 48 h (mL/g DM), corrected for blanks; (ii) A: asymptotic GP (mL/g DM); (iii) T1/2: time when half A is produced (h); (iv) GPMR: maximum predicted GP rate (mL/h); (v) L: lag time (h). A mixed model including laboratories as random effect was used. A significant interaction between substrate and laboratories was found for all variables except A. The most repeatable and reproducible results were observed for A and GP48. The results from this ring test suggest the need for more standardisation, particularly in the procedures that occur outside the laboratory.
UR - http://www.scopus.com/inward/record.url?scp=84877723830&partnerID=8YFLogxK
U2 - 10.1071/AN12091
DO - 10.1071/AN12091
M3 - Journal article
AN - SCOPUS:84877723830
VL - 53
SP - 585
EP - 592
JO - Animal Production Science
JF - Animal Production Science
SN - 1836-0939
IS - 6
ER -
ID: 45859805