Bacteriophage Enumeration and Detection Methods
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Bacteriophage Enumeration and Detection Methods. / Ács, Norbert; Gambino, Michela; Brøndsted, Lone.
In: Frontiers in Microbiology, Vol. 11, 594868, 2020.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Bacteriophage Enumeration and Detection Methods
AU - Ács, Norbert
AU - Gambino, Michela
AU - Brøndsted, Lone
PY - 2020
Y1 - 2020
N2 - Application of phages as alternative antimicrobials to combat pathogenic bacteria and their association to a healthy gut microbiome has prompted a need for precise methods for detection and enumeration of phage particles. There are many applicable methods, but care should be taken considering the measured object (infectious phage, whole phage particle or nucleic acid and proteins) and the concept behind the technique to avoid misinterpretations. While molecular methods cannot discriminate between viable and non-infectious phages, the traditional techniques for counting infectious phages can be time consuming and poorly reproducible. Here, we describe the methods currently used for phage detection and enumeration and highlight their advantages as well as their limitations. Finally, we provide insight on how to deal with complex samples, as well as future prospects in the field of phage quantification.
AB - Application of phages as alternative antimicrobials to combat pathogenic bacteria and their association to a healthy gut microbiome has prompted a need for precise methods for detection and enumeration of phage particles. There are many applicable methods, but care should be taken considering the measured object (infectious phage, whole phage particle or nucleic acid and proteins) and the concept behind the technique to avoid misinterpretations. While molecular methods cannot discriminate between viable and non-infectious phages, the traditional techniques for counting infectious phages can be time consuming and poorly reproducible. Here, we describe the methods currently used for phage detection and enumeration and highlight their advantages as well as their limitations. Finally, we provide insight on how to deal with complex samples, as well as future prospects in the field of phage quantification.
KW - bacteriophage
KW - detection
KW - double agar overlay assay
KW - enumeration
KW - molecular biology
KW - real-time PCR
KW - sequencing
U2 - 10.3389/fmicb.2020.594868
DO - 10.3389/fmicb.2020.594868
M3 - Review
C2 - 33193274
AN - SCOPUS:85095610213
VL - 11
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 594868
ER -
ID: 251586143