Betamethasone Treatment for Atopic Dermatitis in Gut Microbiota Transplanted Mice
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Betamethasone Treatment for Atopic Dermatitis in Gut Microbiota Transplanted Mice. / Debes, Karina P; Evdina, Nathalie A; Laigaard, Ann; Larsen, Julie M; Zachariassen, Line F; Hansen, Camilla H F; Hansen, Axel K.
In: Comparative Medicine, Vol. 70, No. 1, 2020, p. 6-15.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Betamethasone Treatment for Atopic Dermatitis in Gut Microbiota Transplanted Mice
AU - Debes, Karina P
AU - Evdina, Nathalie A
AU - Laigaard, Ann
AU - Larsen, Julie M
AU - Zachariassen, Line F
AU - Hansen, Camilla H F
AU - Hansen, Axel K
PY - 2020
Y1 - 2020
N2 - Gut microbiota composition correlates strongly with essential disease parameters in the oxazolone-induced mouse model for atopic dermatitis. The phenotype of this model can be transferred to germ-free mice with a gut microbiota transplant to achieve high and low responding mice. Therefore, the production of high responding mice through gut microbiota transplantation may be seen as a tool to reduce group sizes or increase power in intervention studies by increasing effect size. We sought to determine whether high responding mice respond to a common treatment in the same way as low responding mice. We hypothesized that while high responding mice would exhibit a higher clinical score than low responding mice before treatment, the clinical parameters would be similar in both groups after betamethasone treatment. Dermatitis was induced with oxazolone in barrier bred Swiss Webster mice, and a high responding and a low responding donor was selected based upon clinical and pathologic scores, as confirmed by monitoring a range of ear tissue cytokines. Feces from these donors were transplanted to pregnant germ-free Swiss Webster dams, and subsequently to their offspring. Although the overall effect of betamethasone on the clinical dermatitis score and ear thickness was rather small, the high responding recipients had significantly higher clinical dermatitis score and ear thickness than the low responding recipients before treatment, and these differences vanished after betamethasone treatment. We conclude that high responding recipients can be treated to a clinical level comparable with the low responding recipients.
AB - Gut microbiota composition correlates strongly with essential disease parameters in the oxazolone-induced mouse model for atopic dermatitis. The phenotype of this model can be transferred to germ-free mice with a gut microbiota transplant to achieve high and low responding mice. Therefore, the production of high responding mice through gut microbiota transplantation may be seen as a tool to reduce group sizes or increase power in intervention studies by increasing effect size. We sought to determine whether high responding mice respond to a common treatment in the same way as low responding mice. We hypothesized that while high responding mice would exhibit a higher clinical score than low responding mice before treatment, the clinical parameters would be similar in both groups after betamethasone treatment. Dermatitis was induced with oxazolone in barrier bred Swiss Webster mice, and a high responding and a low responding donor was selected based upon clinical and pathologic scores, as confirmed by monitoring a range of ear tissue cytokines. Feces from these donors were transplanted to pregnant germ-free Swiss Webster dams, and subsequently to their offspring. Although the overall effect of betamethasone on the clinical dermatitis score and ear thickness was rather small, the high responding recipients had significantly higher clinical dermatitis score and ear thickness than the low responding recipients before treatment, and these differences vanished after betamethasone treatment. We conclude that high responding recipients can be treated to a clinical level comparable with the low responding recipients.
U2 - 10.30802/AALAS-CM-18-000136
DO - 10.30802/AALAS-CM-18-000136
M3 - Journal article
C2 - 31744592
VL - 70
SP - 6
EP - 15
JO - Comparative Medicine
JF - Comparative Medicine
SN - 1532-0820
IS - 1
ER -
ID: 236214981