Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance. / Sørensen, Martine C.Holst; Vitt, Amira; Neve, Horst; Soverini, Matteo; Ahern, Stephen James; Klumpp, Jochen; Brøndsted, Lone.

In: Cell Reports, Vol. 35, No. 10, 109214, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sørensen, MCH, Vitt, A, Neve, H, Soverini, M, Ahern, SJ, Klumpp, J & Brøndsted, L 2021, 'Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance', Cell Reports, vol. 35, no. 10, 109214. https://doi.org/10.1016/j.celrep.2021.109214

APA

Sørensen, M. C. H., Vitt, A., Neve, H., Soverini, M., Ahern, S. J., Klumpp, J., & Brøndsted, L. (2021). Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance. Cell Reports, 35(10), [109214]. https://doi.org/10.1016/j.celrep.2021.109214

Vancouver

Sørensen MCH, Vitt A, Neve H, Soverini M, Ahern SJ, Klumpp J et al. Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance. Cell Reports. 2021;35(10). 109214. https://doi.org/10.1016/j.celrep.2021.109214

Author

Sørensen, Martine C.Holst ; Vitt, Amira ; Neve, Horst ; Soverini, Matteo ; Ahern, Stephen James ; Klumpp, Jochen ; Brøndsted, Lone. / Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance. In: Cell Reports. 2021 ; Vol. 35, No. 10.

Bibtex

@article{4c0aa0820c004020943fcdfe74f309f1,
title = "Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance",
abstract = "Phase variation is a common mechanism for creating phenotypic heterogeneity of surface structures in bacteria important for niche adaptation. In Campylobacter, phase variation occurs by random variation in hypermutable homonucleotide 7–11 G (polyG) tracts. To elucidate how phages adapt to phase-variable hosts, we study Fletchervirus phages infecting Campylobacter dependent on a phase-variable receptor. Our data demonstrate that Fletcherviruses mimic their host and encode hypermutable polyG tracts, leading to phase-variable expression of two of four receptor-binding proteins. This creates phenotypically diverse phage populations, including a sub-population that infects the bacterial host when the phase-variable receptor is not expressed. Such population dynamics of both phage and host promote co-existence in a shared niche. Strikingly, we identify polyG tracts in more than 100 phage genera, infecting more than 70 bacterial species. Future experimental work may confirm phase variation as a widespread strategy for creating phenotypically diverse phage populations.",
keywords = "Campylobacter, Fletchervirus, MeOPN, phage receptor, phage resistance, phages, phase variation, polyG tracts, receptor binding proteins",
author = "S{\o}rensen, {Martine C.Holst} and Amira Vitt and Horst Neve and Matteo Soverini and Ahern, {Stephen James} and Jochen Klumpp and Lone Br{\o}ndsted",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
doi = "10.1016/j.celrep.2021.109214",
language = "English",
volume = "35",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - Campylobacter phages use hypermutable polyG tracts to create phenotypic diversity and evade bacterial resistance

AU - Sørensen, Martine C.Holst

AU - Vitt, Amira

AU - Neve, Horst

AU - Soverini, Matteo

AU - Ahern, Stephen James

AU - Klumpp, Jochen

AU - Brøndsted, Lone

N1 - Publisher Copyright: © 2021 The Authors

PY - 2021

Y1 - 2021

N2 - Phase variation is a common mechanism for creating phenotypic heterogeneity of surface structures in bacteria important for niche adaptation. In Campylobacter, phase variation occurs by random variation in hypermutable homonucleotide 7–11 G (polyG) tracts. To elucidate how phages adapt to phase-variable hosts, we study Fletchervirus phages infecting Campylobacter dependent on a phase-variable receptor. Our data demonstrate that Fletcherviruses mimic their host and encode hypermutable polyG tracts, leading to phase-variable expression of two of four receptor-binding proteins. This creates phenotypically diverse phage populations, including a sub-population that infects the bacterial host when the phase-variable receptor is not expressed. Such population dynamics of both phage and host promote co-existence in a shared niche. Strikingly, we identify polyG tracts in more than 100 phage genera, infecting more than 70 bacterial species. Future experimental work may confirm phase variation as a widespread strategy for creating phenotypically diverse phage populations.

AB - Phase variation is a common mechanism for creating phenotypic heterogeneity of surface structures in bacteria important for niche adaptation. In Campylobacter, phase variation occurs by random variation in hypermutable homonucleotide 7–11 G (polyG) tracts. To elucidate how phages adapt to phase-variable hosts, we study Fletchervirus phages infecting Campylobacter dependent on a phase-variable receptor. Our data demonstrate that Fletcherviruses mimic their host and encode hypermutable polyG tracts, leading to phase-variable expression of two of four receptor-binding proteins. This creates phenotypically diverse phage populations, including a sub-population that infects the bacterial host when the phase-variable receptor is not expressed. Such population dynamics of both phage and host promote co-existence in a shared niche. Strikingly, we identify polyG tracts in more than 100 phage genera, infecting more than 70 bacterial species. Future experimental work may confirm phase variation as a widespread strategy for creating phenotypically diverse phage populations.

KW - Campylobacter

KW - Fletchervirus

KW - MeOPN

KW - phage receptor

KW - phage resistance

KW - phages

KW - phase variation

KW - polyG tracts

KW - receptor binding proteins

U2 - 10.1016/j.celrep.2021.109214

DO - 10.1016/j.celrep.2021.109214

M3 - Journal article

C2 - 34107245

AN - SCOPUS:85107426535

VL - 35

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 10

M1 - 109214

ER -

ID: 272113768