Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice
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Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice. / Zachariassen, Line Fisker; Hansen, Axel Kornerup; Krych, Lukasz; Nielsen, Dennis Sandris; Holm, Thomas Lindebo; Tougaard, Peter; Hansen, Camilla Hartmann Friis.
In: Mucosal Immunology, Vol. 12, No. 6, 2019, p. 1348-1357.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cesarean section increases sensitivity to oxazolone-induced colitis in C57BL/6 mice
AU - Zachariassen, Line Fisker
AU - Hansen, Axel Kornerup
AU - Krych, Lukasz
AU - Nielsen, Dennis Sandris
AU - Holm, Thomas Lindebo
AU - Tougaard, Peter
AU - Hansen, Camilla Hartmann Friis
PY - 2019
Y1 - 2019
N2 - Children born by cesarean section (CS) have an increased risk of developing inflammatory bowel disease (IBD), possibly due to skewed microbial colonization during birth and consequently impaired bacterial stimulation of the developing immune system. The aim of this study was to investigate the association between CS and experimental colitis in a murine model of IBD. It was hypothesized that CS aggravates colonic inflammation due to a change in gut microbiota (GM) composition. C57BL/6 mice, delivered by CS or vaginal delivery (VD), were intra-rectally challenged with oxazolone at 8 weeks of age and monitored for colitis symptoms. The results showed that CS delivered mice experienced an increased body weight loss and colon weight, together with higher colonic concentrations of TNF-α and MPO compared with VD mice. Increased infiltration of inflammatory cells was present in CS delivered mice, as well as a downregulation in expression of the gut integrity genes occludin and tight junction protein 1 indicative of an impaired barrier function. The GM from CS delivered mice without colitis partly contributed to the increase in colitis symptoms when inoculated into germ-free recipient mice. In conclusion, CS increased sensitivity to oxazolone induced colitis in mice.
AB - Children born by cesarean section (CS) have an increased risk of developing inflammatory bowel disease (IBD), possibly due to skewed microbial colonization during birth and consequently impaired bacterial stimulation of the developing immune system. The aim of this study was to investigate the association between CS and experimental colitis in a murine model of IBD. It was hypothesized that CS aggravates colonic inflammation due to a change in gut microbiota (GM) composition. C57BL/6 mice, delivered by CS or vaginal delivery (VD), were intra-rectally challenged with oxazolone at 8 weeks of age and monitored for colitis symptoms. The results showed that CS delivered mice experienced an increased body weight loss and colon weight, together with higher colonic concentrations of TNF-α and MPO compared with VD mice. Increased infiltration of inflammatory cells was present in CS delivered mice, as well as a downregulation in expression of the gut integrity genes occludin and tight junction protein 1 indicative of an impaired barrier function. The GM from CS delivered mice without colitis partly contributed to the increase in colitis symptoms when inoculated into germ-free recipient mice. In conclusion, CS increased sensitivity to oxazolone induced colitis in mice.
U2 - 10.1038/s41385-019-0207-8
DO - 10.1038/s41385-019-0207-8
M3 - Journal article
C2 - 31554900
AN - SCOPUS:85074005748
VL - 12
SP - 1348
EP - 1357
JO - Mucosal Immunology
JF - Mucosal Immunology
SN - 1933-0219
IS - 6
ER -
ID: 230149838