CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design
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SUMMARY: The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and cleavage activation. Careful design is necessary to select a gRNA with high editing efficiency at the on-target site and with minimum off-target potential. Here, we present our webserver for gRNA design with a user-friendly graphical interface, which provides interoperability between our on- and off-target prediction tools, CRISPRon and CRISPRoff, for a complete and streamlined gRNA selection.
AVAILABILITY AND IMPLEMENTATION: The graphical interface uses the Integrative Genomic Viewer (IGV) JavaScript plugin. The backend tools are implemented in Python and C. The CRISPRon and CRISPRoff webservers and command-line tools are freely available at https://rth.dk/resources/crispr.
| Original language | English |
|---|---|
| Journal | Bioinformatics |
| Volume | 38 |
| Issue number | 24 |
| Pages (from-to) | 5437-5439 |
| Number of pages | 3 |
| ISSN | 1367-4811 |
| DOIs | |
| Publication status | Published - 2022 |
Bibliographical note
© The Author(s) 2022. Published by Oxford University Press.
- CRISPR-Cas Systems/genetics, RNA, Guide, CRISPR-Cas Systems, RNA, Guide, Kinetoplastida/genetics, Gene Editing
Research areas
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