Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus. / Yang, Jingxian; Bowring, Janine Zara; Krusche, Janes; Lehmann, Esther; Bejder, Benjamin Svejdal; Silva, Stephanie Fulaz; Bojer, Martin Saxtorph; Grunert, Tom; Peschel, Andreas; Ingmer, Hanne.

In: Cell Reports, Vol. 42, No. 9, 113154, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yang, J, Bowring, JZ, Krusche, J, Lehmann, E, Bejder, BS, Silva, SF, Bojer, MS, Grunert, T, Peschel, A & Ingmer, H 2023, 'Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus', Cell Reports, vol. 42, no. 9, 113154. https://doi.org/10.1016/j.celrep.2023.113154

APA

Yang, J., Bowring, J. Z., Krusche, J., Lehmann, E., Bejder, B. S., Silva, S. F., Bojer, M. S., Grunert, T., Peschel, A., & Ingmer, H. (2023). Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus. Cell Reports, 42(9), [113154]. https://doi.org/10.1016/j.celrep.2023.113154

Vancouver

Yang J, Bowring JZ, Krusche J, Lehmann E, Bejder BS, Silva SF et al. Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus. Cell Reports. 2023;42(9). 113154. https://doi.org/10.1016/j.celrep.2023.113154

Author

Yang, Jingxian ; Bowring, Janine Zara ; Krusche, Janes ; Lehmann, Esther ; Bejder, Benjamin Svejdal ; Silva, Stephanie Fulaz ; Bojer, Martin Saxtorph ; Grunert, Tom ; Peschel, Andreas ; Ingmer, Hanne. / Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus. In: Cell Reports. 2023 ; Vol. 42, No. 9.

Bibtex

@article{a4ea9007787442e8a7a1f0b6d14d8cda,
title = "Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus",
abstract = "Bacteria use quorum sensing (QS) to coordinate group behavior in response to cell density, and some bacterial viruses (phages) also respond to QS. In Staphylococcus aureus, the agr-encoded QS system relies on accumulation of auto-inducing cyclic peptides (AIPs). Other staphylococci also produce AIPs of which many inhibit S. aureus agr. We show that agr induction reduces expression of tarM, encoding a glycosyltransferase responsible for α-N-acetylglucosamine modification of the major S. aureus phage receptor, the wall teichoic acids. This allows lytic phage Stab20 and related phages to infect and kill S. aureus. However, in mixed communities, producers of inhibitory AIPs like S. haemolyticus, S. caprae, and S. pseudintermedius inhibit S. aureus agr, thereby impeding phage infection. Our results demonstrate that cross-species interactions dramatically impact phage susceptibility. These interactions likely influence microbial ecology and impact the efficacy of phages in medical and biotechnological applications such as phage therapy.",
keywords = "agr, CP: Microbiology, glycosylation, phage, Stab20, tarM, tarS, WTA",
author = "Jingxian Yang and Bowring, {Janine Zara} and Janes Krusche and Esther Lehmann and Bejder, {Benjamin Svejdal} and Silva, {Stephanie Fulaz} and Bojer, {Martin Saxtorph} and Tom Grunert and Andreas Peschel and Hanne Ingmer",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
doi = "10.1016/j.celrep.2023.113154",
language = "English",
volume = "42",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "9",

}

RIS

TY - JOUR

T1 - Cross-species communication via agr controls phage susceptibility in Staphylococcus aureus

AU - Yang, Jingxian

AU - Bowring, Janine Zara

AU - Krusche, Janes

AU - Lehmann, Esther

AU - Bejder, Benjamin Svejdal

AU - Silva, Stephanie Fulaz

AU - Bojer, Martin Saxtorph

AU - Grunert, Tom

AU - Peschel, Andreas

AU - Ingmer, Hanne

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023

Y1 - 2023

N2 - Bacteria use quorum sensing (QS) to coordinate group behavior in response to cell density, and some bacterial viruses (phages) also respond to QS. In Staphylococcus aureus, the agr-encoded QS system relies on accumulation of auto-inducing cyclic peptides (AIPs). Other staphylococci also produce AIPs of which many inhibit S. aureus agr. We show that agr induction reduces expression of tarM, encoding a glycosyltransferase responsible for α-N-acetylglucosamine modification of the major S. aureus phage receptor, the wall teichoic acids. This allows lytic phage Stab20 and related phages to infect and kill S. aureus. However, in mixed communities, producers of inhibitory AIPs like S. haemolyticus, S. caprae, and S. pseudintermedius inhibit S. aureus agr, thereby impeding phage infection. Our results demonstrate that cross-species interactions dramatically impact phage susceptibility. These interactions likely influence microbial ecology and impact the efficacy of phages in medical and biotechnological applications such as phage therapy.

AB - Bacteria use quorum sensing (QS) to coordinate group behavior in response to cell density, and some bacterial viruses (phages) also respond to QS. In Staphylococcus aureus, the agr-encoded QS system relies on accumulation of auto-inducing cyclic peptides (AIPs). Other staphylococci also produce AIPs of which many inhibit S. aureus agr. We show that agr induction reduces expression of tarM, encoding a glycosyltransferase responsible for α-N-acetylglucosamine modification of the major S. aureus phage receptor, the wall teichoic acids. This allows lytic phage Stab20 and related phages to infect and kill S. aureus. However, in mixed communities, producers of inhibitory AIPs like S. haemolyticus, S. caprae, and S. pseudintermedius inhibit S. aureus agr, thereby impeding phage infection. Our results demonstrate that cross-species interactions dramatically impact phage susceptibility. These interactions likely influence microbial ecology and impact the efficacy of phages in medical and biotechnological applications such as phage therapy.

KW - agr

KW - CP: Microbiology

KW - glycosylation

KW - phage

KW - Stab20

KW - tarM

KW - tarS

KW - WTA

U2 - 10.1016/j.celrep.2023.113154

DO - 10.1016/j.celrep.2023.113154

M3 - Journal article

C2 - 37725513

AN - SCOPUS:85171385559

VL - 42

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 9

M1 - 113154

ER -

ID: 367714226