TY - JOUR

T1 - Evaluation of two Fasciola hepatica faecal egg counting protocols in sheep and cattle

AU - Reigate, Claire

AU - Williams, Hefin W.

AU - Denwood, Matthew J.

AU - Morphew, Russell M.

AU - Thomas, Eurion R.

AU - Brophy, Peter M.

PY - 2021

Y1 - 2021

N2 - Fascioliasis causes significant economic losses and is a constant challenge to livestock farmers globally. Fluke faecal egg counts (flukeFECs) are a simple, non-invasive method used to detect the presence of patent liver fluke infection. Many flukeFEC techniques exist but they vary in complexity, precision and accuracy. The objective of this study was to evaluate the egg recovery capabilities of two simple flukeFEC methods at different egg concentrations in two ruminant species, using artificially spiked faecal samples. We added Fasciola hepatica eggs to sheep and cattle faeces at 2, 5 10 and 20 epg and utilised the Flukefinder® (FF) and a simple sedimentation method (referred to as the Becker method) to investigate the effects of methods, species and egg density on egg recovery. We calculated the proportion of fluke eggs recovered using each technique, and determined the lowest reliable egg detection threshold of each flukeFEC method. The performance of the flukeFEC methods were also compared using faecal samples collected from naturally infected animals. The egg-spiking study revealed that both FF and the Becker sedimentation method are significantly more likely to recover eggs from cattle faeces than sheep (P < 0.001). Overall, FF recovered more eggs than the Becker method (P < 0.001), and importantly has a reliable low egg detection threshold of 5 epg in sheep and cattle. The kappa coefficient indicated a substantial agreement between FF and the Becker method in naturally infected faecal samples collected from cattle (0.62, P < 0.05) and a moderate agreement in sheep (0.41, P < 0.05). This study demonstrated that FF has a low egg detection threshold and therefore has promising potential for the future of on-farm liver fluke diagnostics.

AB - Fascioliasis causes significant economic losses and is a constant challenge to livestock farmers globally. Fluke faecal egg counts (flukeFECs) are a simple, non-invasive method used to detect the presence of patent liver fluke infection. Many flukeFEC techniques exist but they vary in complexity, precision and accuracy. The objective of this study was to evaluate the egg recovery capabilities of two simple flukeFEC methods at different egg concentrations in two ruminant species, using artificially spiked faecal samples. We added Fasciola hepatica eggs to sheep and cattle faeces at 2, 5 10 and 20 epg and utilised the Flukefinder® (FF) and a simple sedimentation method (referred to as the Becker method) to investigate the effects of methods, species and egg density on egg recovery. We calculated the proportion of fluke eggs recovered using each technique, and determined the lowest reliable egg detection threshold of each flukeFEC method. The performance of the flukeFEC methods were also compared using faecal samples collected from naturally infected animals. The egg-spiking study revealed that both FF and the Becker sedimentation method are significantly more likely to recover eggs from cattle faeces than sheep (P < 0.001). Overall, FF recovered more eggs than the Becker method (P < 0.001), and importantly has a reliable low egg detection threshold of 5 epg in sheep and cattle. The kappa coefficient indicated a substantial agreement between FF and the Becker method in naturally infected faecal samples collected from cattle (0.62, P < 0.05) and a moderate agreement in sheep (0.41, P < 0.05). This study demonstrated that FF has a low egg detection threshold and therefore has promising potential for the future of on-farm liver fluke diagnostics.

KW - Coprology

KW - Fascioliasis

KW - Flukefinder®

KW - Liver fluke

KW - Sedimentation

U2 - 10.1016/j.vetpar.2021.109435

DO - 10.1016/j.vetpar.2021.109435

M3 - Journal article

C2 - 33946031

AN - SCOPUS:85104999210

VL - 294

JO - Veterinary Parasitology

JF - Veterinary Parasitology

SN - 0304-4017

M1 - 109435

ER -