Generation of neural progenitor cells (NPC) from porcine induced pluripotent stem cells (piPSC)
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Generation of neural progenitor cells (NPC) from porcine induced pluripotent stem cells (piPSC). / Machado, Lucas Simões; Pieri, Naira Caroline Godoy; Botigelli, Ramon Cesar; de Castro, Raquel Vasconcelos Guimarães; de Souza, Aline Fernanda; Bridi, Alessandra; Lima, Marina Amaro; Fantinato Neto, Paulo; de Figueiredo Pessôa, Laís Vicari; Martins, Simone Maria Massami Kitamura; de Andrade, André Furugen Cesar; Freude, Kristine Karla; Bressan, Fabiana Fernandes.
In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 14, No. 12, 2020, p. 1880-1891.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Generation of neural progenitor cells (NPC) from porcine induced pluripotent stem cells (piPSC)
AU - Machado, Lucas Simões
AU - Pieri, Naira Caroline Godoy
AU - Botigelli, Ramon Cesar
AU - de Castro, Raquel Vasconcelos Guimarães
AU - de Souza, Aline Fernanda
AU - Bridi, Alessandra
AU - Lima, Marina Amaro
AU - Fantinato Neto, Paulo
AU - de Figueiredo Pessôa, Laís Vicari
AU - Martins, Simone Maria Massami Kitamura
AU - de Andrade, André Furugen Cesar
AU - Freude, Kristine Karla
AU - Bressan, Fabiana Fernandes
N1 - This article is protected by copyright. All rights reserved.
PY - 2020
Y1 - 2020
N2 - In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into iPSC (piPSC) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, β TUBULIN III and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.
AB - In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into iPSC (piPSC) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, β TUBULIN III and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.
U2 - 10.1002/term.3143
DO - 10.1002/term.3143
M3 - Journal article
C2 - 33049106
VL - 14
SP - 1880
EP - 1891
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 12
ER -
ID: 250120902