Genome-wide alternation and effect of DNA methylation in the impairments of steroidogenesis and spermatogenesis after PM2.5 exposure
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Genome-wide alternation and effect of DNA methylation in the impairments of steroidogenesis and spermatogenesis after PM2.5 exposure. / Zhang, Zhonghao; Wang, Jiankang; Shi, Fuquan; Li, Yingqing; Zou, Peng; Tang, Ying; Liu, Chang; Wang, Yimeng; Ling, Xi; Sun, Lei; Liu, Cuiqing; Zhang, Yanshu; Gao, Fei; Chen, Qing; Ao, Lin; Han, Fei; Liu, Jinyi; Cao, Jia.
In: Environment International, Vol. 169, 107544, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Genome-wide alternation and effect of DNA methylation in the impairments of steroidogenesis and spermatogenesis after PM2.5 exposure
AU - Zhang, Zhonghao
AU - Wang, Jiankang
AU - Shi, Fuquan
AU - Li, Yingqing
AU - Zou, Peng
AU - Tang, Ying
AU - Liu, Chang
AU - Wang, Yimeng
AU - Ling, Xi
AU - Sun, Lei
AU - Liu, Cuiqing
AU - Zhang, Yanshu
AU - Gao, Fei
AU - Chen, Qing
AU - Ao, Lin
AU - Han, Fei
AU - Liu, Jinyi
AU - Cao, Jia
N1 - Publisher Copyright: © 2022
PY - 2022
Y1 - 2022
N2 - The effects of ambient fine particles on male reproductive health have raised widespread concern. The particular underlying mechanisms of the damage remain largely unclear and demand more research in new directions. Previous research has revealed that DNA methylation plays an important role in male reproductive development and is also vulnerable to environmental influences. However, there hasn't been enough investigation into the involvement of DNA methylation in PM2.5-induced male reproductive toxicity. Here, we establish a real-time PM2.5 exposure model and revealed that PM2.5 exposure could lead to testicular dysfunction including spermatogenesis impairment and steroid hormone dysfunction. In particular, the decrease in the testicular global level of 5-methylcytosine (5mC) indicated a possible association of DNA methylation with testicular injury induced by PM2.5 exposure. Further genome-wide methylation analysis revealed genomic hypomethylation of testicular DNA and identified more than 1000 differentially methylated regions in both CAP and UA versus FA, indicating that PM2.5 exposure, even low-dose, could modulate the testicular methylome. Furthermore, integrated analysis of methylome and transcriptome identified some key methylated genes and networks, which may be involved in spermatogenesis and synthesis of steroid hormone. The testicular methylation levels of key genes especially Cyp11a1 and Pax8 raised, and their consequent reduced expression may impair the testosterone and sperm production process. Our research provides fundamental knowledge as well as novel insights into the possible involvement of DNA methylation in PM2.5-induced male reproductive harm.
AB - The effects of ambient fine particles on male reproductive health have raised widespread concern. The particular underlying mechanisms of the damage remain largely unclear and demand more research in new directions. Previous research has revealed that DNA methylation plays an important role in male reproductive development and is also vulnerable to environmental influences. However, there hasn't been enough investigation into the involvement of DNA methylation in PM2.5-induced male reproductive toxicity. Here, we establish a real-time PM2.5 exposure model and revealed that PM2.5 exposure could lead to testicular dysfunction including spermatogenesis impairment and steroid hormone dysfunction. In particular, the decrease in the testicular global level of 5-methylcytosine (5mC) indicated a possible association of DNA methylation with testicular injury induced by PM2.5 exposure. Further genome-wide methylation analysis revealed genomic hypomethylation of testicular DNA and identified more than 1000 differentially methylated regions in both CAP and UA versus FA, indicating that PM2.5 exposure, even low-dose, could modulate the testicular methylome. Furthermore, integrated analysis of methylome and transcriptome identified some key methylated genes and networks, which may be involved in spermatogenesis and synthesis of steroid hormone. The testicular methylation levels of key genes especially Cyp11a1 and Pax8 raised, and their consequent reduced expression may impair the testosterone and sperm production process. Our research provides fundamental knowledge as well as novel insights into the possible involvement of DNA methylation in PM2.5-induced male reproductive harm.
KW - DNA methylome
KW - Male reproductive system
KW - PM
KW - Spermatogenesis
U2 - 10.1016/j.envint.2022.107544
DO - 10.1016/j.envint.2022.107544
M3 - Journal article
C2 - 36174482
AN - SCOPUS:85138804390
VL - 169
JO - Environment international
JF - Environment international
SN - 0160-4120
M1 - 107544
ER -
ID: 322644629