Gluten-free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring
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Gluten-free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring. / Haupt-Jorgensen, Martin; Larsen, Jesper; Josefsen, Knud; Jørgensen, Tina Z.; Antvorskov, Julie Christine; Hansen, Axel K.; Buschard, Karsten.
In: Diabetes - Metabolism: Research and Reviews (Print Edition), Vol. 34, No. 4, e2987, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Gluten-free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring
AU - Haupt-Jorgensen, Martin
AU - Larsen, Jesper
AU - Josefsen, Knud
AU - Jørgensen, Tina Z.
AU - Antvorskov, Julie Christine
AU - Hansen, Axel K.
AU - Buschard, Karsten
PY - 2018
Y1 - 2018
N2 - Background: Gluten-free (GF) diet during pregnancy ameliorates autoimmune diabetes in nonobese diabetic (NOD) mouse offspring. Due to comorbidity of celiac disease in type 1 diabetes, we hypothesized that GF diet in utero alleviates the humoral and histopathological signs of celiac disease in NOD mice. We aimed to establish the mechanisms behind the diabetes-protective effect of GF diet in utero. Methods: Breeding pairs of NOD mice were fed a GF or gluten-containing standard (STD) diet until parturition. The offspring were nursed by mothers on STD diet and continued on this diet until ages 4 and 13 weeks. Analyses of serum antitissue transglutaminase (anti-tTG) intestine and islet histology, islet transglutaminase (TG) activity, and cytokine expression in T cells from lymphoid organs were performed. Results: GF versus STD diet in utero led to reduced serum anti-tTG titre and increased villus-to-crypt ratio at both ages. Insulitis along with systemic and local inflammation were decreased, but islet TG activity was unchanged in 13-week-old GF mice. These mice had unchanged beta-cell volumes, but increased islet numbers throughout the prediabetic period. Conclusions: Collectively, GF diet administered during pregnancy improves signs of celiac disease and autoimmune diabetes in the offspring. The diabetes-ameliorative effect of GF diet in utero is followed by dampening of inflammation, unchanged beta-cell volume, but increased islet numbers.
AB - Background: Gluten-free (GF) diet during pregnancy ameliorates autoimmune diabetes in nonobese diabetic (NOD) mouse offspring. Due to comorbidity of celiac disease in type 1 diabetes, we hypothesized that GF diet in utero alleviates the humoral and histopathological signs of celiac disease in NOD mice. We aimed to establish the mechanisms behind the diabetes-protective effect of GF diet in utero. Methods: Breeding pairs of NOD mice were fed a GF or gluten-containing standard (STD) diet until parturition. The offspring were nursed by mothers on STD diet and continued on this diet until ages 4 and 13 weeks. Analyses of serum antitissue transglutaminase (anti-tTG) intestine and islet histology, islet transglutaminase (TG) activity, and cytokine expression in T cells from lymphoid organs were performed. Results: GF versus STD diet in utero led to reduced serum anti-tTG titre and increased villus-to-crypt ratio at both ages. Insulitis along with systemic and local inflammation were decreased, but islet TG activity was unchanged in 13-week-old GF mice. These mice had unchanged beta-cell volumes, but increased islet numbers throughout the prediabetic period. Conclusions: Collectively, GF diet administered during pregnancy improves signs of celiac disease and autoimmune diabetes in the offspring. The diabetes-ameliorative effect of GF diet in utero is followed by dampening of inflammation, unchanged beta-cell volume, but increased islet numbers.
KW - beta-cell
KW - celiac disease
KW - gluten-free diet
KW - islet of Langerhans
KW - NOD mice
KW - type 1 diabetes
U2 - 10.1002/dmrr.2987
DO - 10.1002/dmrr.2987
M3 - Journal article
C2 - 29392873
AN - SCOPUS:85046373339
VL - 34
JO - Diabetes/Metabolism Research and Reviews
JF - Diabetes/Metabolism Research and Reviews
SN - 1520-7552
IS - 4
M1 - e2987
ER -
ID: 202028221