Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection

Research output: Contribution to journalJournal articleResearchpeer-review

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Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection. / Zuo, Shaozhi; Karami, Asma M.; Ødegård, Jørgen; Mathiessen, Heidi; Marana, Moonika H.; Jaafar, Rzgar M.; von Gersdorff Jørgensen, Louise; Abdu, Mohamed; Kania, Per W.; Dalsgaard, Inger; Nielsen, Torben; Buchmann, Kurt.

In: Fish and Shellfish Immunology, Vol. 106, 2020, p. 441-450.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Zuo, S, Karami, AM, Ødegård, J, Mathiessen, H, Marana, MH, Jaafar, RM, von Gersdorff Jørgensen, L, Abdu, M, Kania, PW, Dalsgaard, I, Nielsen, T & Buchmann, K 2020, 'Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection', Fish and Shellfish Immunology, vol. 106, pp. 441-450. https://doi.org/10.1016/j.fsi.2020.07.023

APA

Zuo, S., Karami, A. M., Ødegård, J., Mathiessen, H., Marana, M. H., Jaafar, R. M., von Gersdorff Jørgensen, L., Abdu, M., Kania, P. W., Dalsgaard, I., Nielsen, T., & Buchmann, K. (2020). Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection. Fish and Shellfish Immunology, 106, 441-450. https://doi.org/10.1016/j.fsi.2020.07.023

Vancouver

Zuo S, Karami AM, Ødegård J, Mathiessen H, Marana MH, Jaafar RM et al. Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection. Fish and Shellfish Immunology. 2020;106:441-450. https://doi.org/10.1016/j.fsi.2020.07.023

Author

Zuo, Shaozhi ; Karami, Asma M. ; Ødegård, Jørgen ; Mathiessen, Heidi ; Marana, Moonika H. ; Jaafar, Rzgar M. ; von Gersdorff Jørgensen, Louise ; Abdu, Mohamed ; Kania, Per W. ; Dalsgaard, Inger ; Nielsen, Torben ; Buchmann, Kurt. / Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection. In: Fish and Shellfish Immunology. 2020 ; Vol. 106. pp. 441-450.

Bibtex

@article{ef756ea2503d478998b09cb84bd726a3,
title = "Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection",
abstract = "Selective breeding programmes involving marker assisted selection of innately pathogen resistant strains of rainbow trout rely on reliable controlled infection studies, extensive DNA typing of individual fish and recording of expression of relevant genes. We exposed juvenile rainbow trout (6 h bath to 2.6 × 105 CFU mL−1) to the fish pathogen Yersinia ruckeri serotype O1, biotype 2, eliciting Enteric Red Mouth Disease ERM, and followed the disease progression over 21 days. Cumulative mortality reached 42% at 12 days post challenge (dpc) after which no disease signs were recorded. All fish were sampled for DNA-typing (50 k SNP chip, Affymetrix{\textregistered}) throughout the course of infection when they showed clinical signs of disease (susceptible fish) or at day 21 when fish showed no clinical signs of disease (survivors - resistant fish). Genome-wide association analyses of 1027 trout applying single nucleotide polymorphisms (SNPs) as markers revealed an association between traits (susceptible/resistant) and certain regions of the trout genome. It was indicated that multiple genes are involved in rainbow trout resistance towards ERM whereby it is considered a polygenic trait. A corresponding trout group was kept as non-exposed controls and a comparative expression analysis of central innate and adaptive immune genes in gills, spleen and liver was performed for three fish groups: 1) moribund trout exhibiting clinical signs 7 dpc (CS), 2) exposed fish without clinical signs at the same sampling point (NCS) and 3) surviving fish at 21 dpc (survivors). Immune genes encoding inflammatory cytokines (IL-1β, IL-2A, IL-6A, IL-8, IL-10A, IL-12, IL-17A/F2A, IL-17C1, IL-17C2, IL-22, IFNγ, TNFα), acute phase reactants (SAA, C3, cathelicidins, lysozyme) were expressed differently in CS and NCS fish. Correlation (negative or positive) between expression of genes and bacterial load suggested involvement of immune genes in protection. Down-regulation of adaptive immune genes including IgDm, IgDs, IgT and TCR-β was seen primarily in CS and NCS fish whereas survivors showed up-regulation of effector molecule genes such as cathelicidins, complement and lysozyme suggesting their role in clearing the infection. In conclusion, SNP analyses indicated that ERM resistance in rainbow trout is a multi-locus trait. The gene expression in surviving fish suggested that several immune genes are associated with the trait conferring resistance.",
keywords = "Heritability, Immune gene expressions, Rainbow trout, Selective breeding, Yersinia ruckeri",
author = "Shaozhi Zuo and Karami, {Asma M.} and J{\o}rgen {\O}deg{\aa}rd and Heidi Mathiessen and Marana, {Moonika H.} and Jaafar, {Rzgar M.} and {von Gersdorff J{\o}rgensen}, Louise and Mohamed Abdu and Kania, {Per W.} and Inger Dalsgaard and Torben Nielsen and Kurt Buchmann",
year = "2020",
doi = "10.1016/j.fsi.2020.07.023",
language = "English",
volume = "106",
pages = "441--450",
journal = "Fish and Shellfish Immunology",
issn = "1050-4648",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection

AU - Zuo, Shaozhi

AU - Karami, Asma M.

AU - Ødegård, Jørgen

AU - Mathiessen, Heidi

AU - Marana, Moonika H.

AU - Jaafar, Rzgar M.

AU - von Gersdorff Jørgensen, Louise

AU - Abdu, Mohamed

AU - Kania, Per W.

AU - Dalsgaard, Inger

AU - Nielsen, Torben

AU - Buchmann, Kurt

PY - 2020

Y1 - 2020

N2 - Selective breeding programmes involving marker assisted selection of innately pathogen resistant strains of rainbow trout rely on reliable controlled infection studies, extensive DNA typing of individual fish and recording of expression of relevant genes. We exposed juvenile rainbow trout (6 h bath to 2.6 × 105 CFU mL−1) to the fish pathogen Yersinia ruckeri serotype O1, biotype 2, eliciting Enteric Red Mouth Disease ERM, and followed the disease progression over 21 days. Cumulative mortality reached 42% at 12 days post challenge (dpc) after which no disease signs were recorded. All fish were sampled for DNA-typing (50 k SNP chip, Affymetrix®) throughout the course of infection when they showed clinical signs of disease (susceptible fish) or at day 21 when fish showed no clinical signs of disease (survivors - resistant fish). Genome-wide association analyses of 1027 trout applying single nucleotide polymorphisms (SNPs) as markers revealed an association between traits (susceptible/resistant) and certain regions of the trout genome. It was indicated that multiple genes are involved in rainbow trout resistance towards ERM whereby it is considered a polygenic trait. A corresponding trout group was kept as non-exposed controls and a comparative expression analysis of central innate and adaptive immune genes in gills, spleen and liver was performed for three fish groups: 1) moribund trout exhibiting clinical signs 7 dpc (CS), 2) exposed fish without clinical signs at the same sampling point (NCS) and 3) surviving fish at 21 dpc (survivors). Immune genes encoding inflammatory cytokines (IL-1β, IL-2A, IL-6A, IL-8, IL-10A, IL-12, IL-17A/F2A, IL-17C1, IL-17C2, IL-22, IFNγ, TNFα), acute phase reactants (SAA, C3, cathelicidins, lysozyme) were expressed differently in CS and NCS fish. Correlation (negative or positive) between expression of genes and bacterial load suggested involvement of immune genes in protection. Down-regulation of adaptive immune genes including IgDm, IgDs, IgT and TCR-β was seen primarily in CS and NCS fish whereas survivors showed up-regulation of effector molecule genes such as cathelicidins, complement and lysozyme suggesting their role in clearing the infection. In conclusion, SNP analyses indicated that ERM resistance in rainbow trout is a multi-locus trait. The gene expression in surviving fish suggested that several immune genes are associated with the trait conferring resistance.

AB - Selective breeding programmes involving marker assisted selection of innately pathogen resistant strains of rainbow trout rely on reliable controlled infection studies, extensive DNA typing of individual fish and recording of expression of relevant genes. We exposed juvenile rainbow trout (6 h bath to 2.6 × 105 CFU mL−1) to the fish pathogen Yersinia ruckeri serotype O1, biotype 2, eliciting Enteric Red Mouth Disease ERM, and followed the disease progression over 21 days. Cumulative mortality reached 42% at 12 days post challenge (dpc) after which no disease signs were recorded. All fish were sampled for DNA-typing (50 k SNP chip, Affymetrix®) throughout the course of infection when they showed clinical signs of disease (susceptible fish) or at day 21 when fish showed no clinical signs of disease (survivors - resistant fish). Genome-wide association analyses of 1027 trout applying single nucleotide polymorphisms (SNPs) as markers revealed an association between traits (susceptible/resistant) and certain regions of the trout genome. It was indicated that multiple genes are involved in rainbow trout resistance towards ERM whereby it is considered a polygenic trait. A corresponding trout group was kept as non-exposed controls and a comparative expression analysis of central innate and adaptive immune genes in gills, spleen and liver was performed for three fish groups: 1) moribund trout exhibiting clinical signs 7 dpc (CS), 2) exposed fish without clinical signs at the same sampling point (NCS) and 3) surviving fish at 21 dpc (survivors). Immune genes encoding inflammatory cytokines (IL-1β, IL-2A, IL-6A, IL-8, IL-10A, IL-12, IL-17A/F2A, IL-17C1, IL-17C2, IL-22, IFNγ, TNFα), acute phase reactants (SAA, C3, cathelicidins, lysozyme) were expressed differently in CS and NCS fish. Correlation (negative or positive) between expression of genes and bacterial load suggested involvement of immune genes in protection. Down-regulation of adaptive immune genes including IgDm, IgDs, IgT and TCR-β was seen primarily in CS and NCS fish whereas survivors showed up-regulation of effector molecule genes such as cathelicidins, complement and lysozyme suggesting their role in clearing the infection. In conclusion, SNP analyses indicated that ERM resistance in rainbow trout is a multi-locus trait. The gene expression in surviving fish suggested that several immune genes are associated with the trait conferring resistance.

KW - Heritability

KW - Immune gene expressions

KW - Rainbow trout

KW - Selective breeding

KW - Yersinia ruckeri

U2 - 10.1016/j.fsi.2020.07.023

DO - 10.1016/j.fsi.2020.07.023

M3 - Journal article

C2 - 32791094

AN - SCOPUS:85089485824

VL - 106

SP - 441

EP - 450

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -

ID: 248147352