Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells
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Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells. / Koch, Thomas Gadegaard; Thomsen, Preben Dybdahl; Betts, Dean H.
In: Cytotherapy, Vol. 11, No. 4, 2009, p. 443-447.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells
AU - Koch, Thomas Gadegaard
AU - Thomsen, Preben Dybdahl
AU - Betts, Dean H.
PY - 2009
Y1 - 2009
N2 - BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.
AB - BACKGROUND AIMS: A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. METHODS: Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS AND CONCLUSIONS: Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.
U2 - 10.1080/14653240902887259
DO - 10.1080/14653240902887259
M3 - Journal article
C2 - 19513899
VL - 11
SP - 443
EP - 447
JO - Cytotherapy
JF - Cytotherapy
SN - 1465-3249
IS - 4
ER -
ID: 14303884