In vitro cell cultures of Brunner's glands from male mouse to study GLP-1 receptor function
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In vitro cell cultures of Brunner's glands from male mouse to study GLP-1 receptor function. / Voetmann, Louise M.; Underwood, Christina R.; Rolin, Bidda; Hansen, Axel K.; Kirk, Rikke K.; Pyke, Charles; Knudsen, Lotte B.; Frederiksen, Klaus S.
In: American journal of physiology. Cell physiology, Vol. 322, No. 6, 2022, p. C1260-C1269.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - In vitro cell cultures of Brunner's glands from male mouse to study GLP-1 receptor function
AU - Voetmann, Louise M.
AU - Underwood, Christina R.
AU - Rolin, Bidda
AU - Hansen, Axel K.
AU - Kirk, Rikke K.
AU - Pyke, Charles
AU - Knudsen, Lotte B.
AU - Frederiksen, Klaus S.
PY - 2022
Y1 - 2022
N2 - Exocrine glands in the submucosa of the proximal duodenum secrete alkaline fluid containing mucus to protect the intestinal mucosa from acidic stomach contents. These glands, known as Brunner's glands, express high glucagon-like peptide 1 receptor (GLP-1R) levels. Previous studies have suggested that activation of the GLP-1R induces expression of barrier protective genes in Brunner's glands. Still, the lack of a viable in vitro culture of Brunner's glands has hampered additional studies of the functional consequences of GLP-1R activation. In this study, we established a procedure to isolate and culture cells derived from murine Brunner's glands. The isolated glandular cells retained functional GLP-1R expression in culture, making this in vitro system suitable for the study of GLP-1R activation. We found that cells derived from the Brunner's glands of mice pretreated with semaglutide contained significantly more mucus compared with Brunner's glands from vehicle-treated mice. Our data suggest a protective intestinal response upon semaglutide treatment, but further studies are required to leverage the full potential of cultured Brunner's gland cells.
AB - Exocrine glands in the submucosa of the proximal duodenum secrete alkaline fluid containing mucus to protect the intestinal mucosa from acidic stomach contents. These glands, known as Brunner's glands, express high glucagon-like peptide 1 receptor (GLP-1R) levels. Previous studies have suggested that activation of the GLP-1R induces expression of barrier protective genes in Brunner's glands. Still, the lack of a viable in vitro culture of Brunner's glands has hampered additional studies of the functional consequences of GLP-1R activation. In this study, we established a procedure to isolate and culture cells derived from murine Brunner's glands. The isolated glandular cells retained functional GLP-1R expression in culture, making this in vitro system suitable for the study of GLP-1R activation. We found that cells derived from the Brunner's glands of mice pretreated with semaglutide contained significantly more mucus compared with Brunner's glands from vehicle-treated mice. Our data suggest a protective intestinal response upon semaglutide treatment, but further studies are required to leverage the full potential of cultured Brunner's gland cells.
KW - Brunner’s glands
KW - GLP-1
KW - gut
KW - in vitro
KW - mouse
KW - mucus
U2 - 10.1152/ajpcell.00345.2021
DO - 10.1152/ajpcell.00345.2021
M3 - Journal article
C2 - 35442827
AN - SCOPUS:85131702214
VL - 322
SP - C1260-C1269
JO - American Journal of Physiology: Cell Physiology
JF - American Journal of Physiology: Cell Physiology
SN - 0363-6143
IS - 6
ER -
ID: 311121779