Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats. / Thøgersen, Rebekka; Castro-Mejía, Josué L.; Sundekilde, Ulrik Kræmer; H. Hansen, Lars; Gray, Nicola; Kuhnle, Gunter; Rye Jørgensen, Niklas; Hansen, Axel Kornerup; Nielsen, Dennis Sandris; Bertram, Hanne Christine.

In: Food Chemistry, Vol. 331, 127291, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Thøgersen, R, Castro-Mejía, JL, Sundekilde, UK, H. Hansen, L, Gray, N, Kuhnle, G, Rye Jørgensen, N, Hansen, AK, Nielsen, DS & Bertram, HC 2020, 'Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats', Food Chemistry, vol. 331, 127291. https://doi.org/10.1016/j.foodchem.2020.127291

APA

Thøgersen, R., Castro-Mejía, J. L., Sundekilde, U. K., H. Hansen, L., Gray, N., Kuhnle, G., Rye Jørgensen, N., Hansen, A. K., Nielsen, D. S., & Bertram, H. C. (2020). Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats. Food Chemistry, 331, [127291]. https://doi.org/10.1016/j.foodchem.2020.127291

Vancouver

Thøgersen R, Castro-Mejía JL, Sundekilde UK, H. Hansen L, Gray N, Kuhnle G et al. Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats. Food Chemistry. 2020;331. 127291. https://doi.org/10.1016/j.foodchem.2020.127291

Author

Thøgersen, Rebekka ; Castro-Mejía, Josué L. ; Sundekilde, Ulrik Kræmer ; H. Hansen, Lars ; Gray, Nicola ; Kuhnle, Gunter ; Rye Jørgensen, Niklas ; Hansen, Axel Kornerup ; Nielsen, Dennis Sandris ; Bertram, Hanne Christine. / Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats. In: Food Chemistry. 2020 ; Vol. 331.

Bibtex

@article{d876ebdb7fa54474a3b2cede0d6046b6,
title = "Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats",
abstract = "This study investigated inulin and calcium-rich milk mineral incorporation into a pork sausage in order to examine the effects on microbiome and biochemical activity in the gastrointestinal tract upon ingestion. Rats (n = 48) were fed one of four sausages; a pork sausage enriched with 1) inulin (6.0%) and milk mineral (3%), 2) inulin (6.0%), 3) milk mineral (3%) or 4) control sausages without enrichment. NMR-based metabolomics revealed that inulin-enrichment increased the fecal concentration of short-chain fatty acids (SCFAs). Milk mineral-enrichment also increased SCFA concentrations, although less pronounced. In addition, milk mineral reduced the concentration of nitroso compounds in feces and small intestinal content. Combined enrichment with both inulin and milk mineral showed no cumulative effect on SCFA formation and seemed to oppose the milk mineral-induced reduction of nitroso compound formation. 16S rRNA gene amplicon sequencing indicated that alterations of the gut microbiome contributed to the observed effects.",
keywords = "Dietary fiber fortification, Gut microbiota, Metabolomics, Milk mineral fortification, Nitroso compounds, Processed meat",
author = "Rebekka Th{\o}gersen and Castro-Mej{\'i}a, {Josu{\'e} L.} and Sundekilde, {Ulrik Kr{\ae}mer} and {H. Hansen}, Lars and Nicola Gray and Gunter Kuhnle and {Rye J{\o}rgensen}, Niklas and Hansen, {Axel Kornerup} and Nielsen, {Dennis Sandris} and Bertram, {Hanne Christine}",
year = "2020",
doi = "10.1016/j.foodchem.2020.127291",
language = "English",
volume = "331",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Inulin and milk mineral fortification of a pork sausage exhibits distinct effects on the microbiome and biochemical activity in the gut of healthy rats

AU - Thøgersen, Rebekka

AU - Castro-Mejía, Josué L.

AU - Sundekilde, Ulrik Kræmer

AU - H. Hansen, Lars

AU - Gray, Nicola

AU - Kuhnle, Gunter

AU - Rye Jørgensen, Niklas

AU - Hansen, Axel Kornerup

AU - Nielsen, Dennis Sandris

AU - Bertram, Hanne Christine

PY - 2020

Y1 - 2020

N2 - This study investigated inulin and calcium-rich milk mineral incorporation into a pork sausage in order to examine the effects on microbiome and biochemical activity in the gastrointestinal tract upon ingestion. Rats (n = 48) were fed one of four sausages; a pork sausage enriched with 1) inulin (6.0%) and milk mineral (3%), 2) inulin (6.0%), 3) milk mineral (3%) or 4) control sausages without enrichment. NMR-based metabolomics revealed that inulin-enrichment increased the fecal concentration of short-chain fatty acids (SCFAs). Milk mineral-enrichment also increased SCFA concentrations, although less pronounced. In addition, milk mineral reduced the concentration of nitroso compounds in feces and small intestinal content. Combined enrichment with both inulin and milk mineral showed no cumulative effect on SCFA formation and seemed to oppose the milk mineral-induced reduction of nitroso compound formation. 16S rRNA gene amplicon sequencing indicated that alterations of the gut microbiome contributed to the observed effects.

AB - This study investigated inulin and calcium-rich milk mineral incorporation into a pork sausage in order to examine the effects on microbiome and biochemical activity in the gastrointestinal tract upon ingestion. Rats (n = 48) were fed one of four sausages; a pork sausage enriched with 1) inulin (6.0%) and milk mineral (3%), 2) inulin (6.0%), 3) milk mineral (3%) or 4) control sausages without enrichment. NMR-based metabolomics revealed that inulin-enrichment increased the fecal concentration of short-chain fatty acids (SCFAs). Milk mineral-enrichment also increased SCFA concentrations, although less pronounced. In addition, milk mineral reduced the concentration of nitroso compounds in feces and small intestinal content. Combined enrichment with both inulin and milk mineral showed no cumulative effect on SCFA formation and seemed to oppose the milk mineral-induced reduction of nitroso compound formation. 16S rRNA gene amplicon sequencing indicated that alterations of the gut microbiome contributed to the observed effects.

KW - Dietary fiber fortification

KW - Gut microbiota

KW - Metabolomics

KW - Milk mineral fortification

KW - Nitroso compounds

KW - Processed meat

U2 - 10.1016/j.foodchem.2020.127291

DO - 10.1016/j.foodchem.2020.127291

M3 - Journal article

C2 - 32559598

AN - SCOPUS:85086462582

VL - 331

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

M1 - 127291

ER -

ID: 243339022