Lymph Nodes Draining Infections Investigated by PET and Immunohistochemistry in a Juvenile Porcine Model
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Background: [18 F]FDG and [11 C]methionine accumulate in lymph nodes draining S. aureus-infected foci. The lymph nodes were characterized by weight, [11 C]methionine-and [18 F]FDG-positron emissions tomography (PET)/computed tomography (CT), and immunohistochemical (IHC)-staining. Methods: 20 pigs inoculated with S. aureus into the right femoral artery were PET/CT-scanned with [18 F]FDG, and nine of the pigs were additionally scanned with [11 C]methionine. Mammary, medial iliac, and popliteal lymph nodes from the left and right hind limbs were weighed. IHC-staining for calculations of area fractions of Ki-67, L1, and IL-8 positive cells was done in mammary and popliteal lymph nodes from the nine pigs. Results: The pigs developed one to six osteomyelitis foci. Some pigs developed contiguous infections of peri-osseous tissue and inoculationsite abscesses. Weights of mammary and medial iliac lymph nodes and their [18 F]FDG maximum Standardized Uptake Values (SUVFDGmax) showed a significant increase in the inoculated limb compared to the left limb. Popliteal lymph node weight and their FDG uptake did not differ significantly between hind limbs. Area fractions of Ki-67 and IL-8 in the right mammary lymph nodes and SUVMetmax in the right popliteal lymph nodes were significantly increased compared with the left side. Conclusion: The PET-tracers [18 F]FDG and [11 C]methionine, and the IHC-markers Ki-67 and IL-8, but not L1, showed increased values in lymph nodes draining soft tissues infected with S. aureus. The increase in [11 C]methionine may indicate a more acute lymph node response, whereas an increase in [18 F]FDG may indicate a more chronic response.
Original language | English |
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Article number | 2792 |
Journal | Molecules |
Volume | 27 |
Issue number | 9 |
ISSN | 1420-3049 |
DOIs | |
Publication status | Published - 2022 |
Bibliographical note
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
- calcium-binding leukocyte L1, IL-8, immunohistochemistry, Ki-67, lymph nodes, PET, pig model, Staphylococcus aureus, [ C]methionine, [ F]FDG
Research areas
ID: 308121925